About

• Generates random barcode sequences for indexing/barcoding/NGS applications
• Random barcodes generated can be of specified length & within specified GC% ranges

Pre-Requisites

Install python modules random and argparse

pip install argparse
pip install random

Usage

python Barcoder.py [-h] -l LENGTH -min GC_MIN -max GC_MAX [-n NUM_BARCODES]

Note: Recommendation for -min is 0.45 and -max is 0.55 for generation of balanced barcodes

Note: For any generated barcodes, it is important to cross-check barcodes for any existing DNA motifs, restriction enzyme sites or other functionally relevant DNA sequences by using appropriate databases JASPAR, NEBcutter, depending on the purpose and design of the barcode containing sequences.