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usage.md aktualisieren
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Co-authored-by: Anabella Trigila <[email protected]>
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apeltzer and atrigila authored Oct 7, 2024
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Expand Up @@ -109,7 +109,7 @@ CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz
The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet must have at least 2 columns (`sample` and `fastq1`). A third column can be added if the sample is paired-end (`fastq2`).

> [!NOTE]
> Most of the tools used can't accommodate paired end reads, so whenever paired-end samples are used as inputs, the R1 and R2 reads are concatenated into a single fastq file by the pipeline.
> Most of the tools used can't accommodate paired end reads, so whenever paired-end samples are used as inputs, only the R1 files are used by the pipeline.
A final samplesheet file consisting of single-end data and may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice.

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