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FEAT: UPPMAX Claim profile responsibility + add rnaseq config #1097
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3a28f01
Claim profile responsibility
maxulysse 6ea3f98
add rnaseq pipeline UPPMAX specific config
maxulysse bb78983
first POC
maxulysse a5fc841
include params file for pico
maxulysse 61b23ad
Merge branch 'master' into uppmax_miarka_rnaseq
maxulysse 3dc18fb
simpler params files
maxulysse c276b76
add docs
maxulysse aa6378e
Finalize uppmax rnaseq configuration
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| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -0,0 +1,11 @@ | ||
| // NGI Stockholm specific configurations for rnaseq on UPPMAX (miarka) | ||
| // Genome overrides are triggered by the SITE environment variable | ||
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| params { | ||
| config_profile_description = System.getenv('SITE') == 'NGI-S' | ||
| ? 'nf-core/rnaseq UPPMAX cluster profile provided by nf-core/configs.' | ||
| : 'UPPMAX (Bianca) cluster profile provided by nf-core/configs.' | ||
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| gencode = (System.getenv('SITE') == 'NGI-S' && params.genome == 'GRCh38') | ||
| save_reference = (System.getenv('SITE') == 'NGI-S' && params.genome == 'GRCh38') | ||
| } |
5 changes: 5 additions & 0 deletions
5
conf/pipeline/rnaseq/uppmax/rnaseq_sthlm_Pico-V3-trim_UMIs_params.yaml
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| extra_trimgalore_args: "--clip_r2 14" | ||
| genomes: | ||
| GRCh38: | ||
| bed12: "/proj/ngi2016003/nobackup/junw/RNAseq/genes_GRCh38.bed" | ||
| gtf: "/sw/data/uppnex/igenomes/Homo_sapiens/NCBI/GRCh38/Annotation/Genes.gencode/genes.gtf" |
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| Original file line number | Diff line number | Diff line change |
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| genomes: | ||
| GRCh38: | ||
| bed12: "/proj/ngi2016003/nobackup/junw/RNAseq/genes_GRCh38.bed" | ||
| gtf: "/sw/data/uppnex/igenomes/Homo_sapiens/NCBI/GRCh38/Annotation/Genes.gencode/genes.gtf" | ||
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| Original file line number | Diff line number | Diff line change |
|---|---|---|
| @@ -0,0 +1,28 @@ | ||
| # nf-core/configs: uppmax rnaseq specific configuration | ||
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| ## NGI Stockholm specific configurations for uppmax | ||
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| When the `SITE` environment variable is set to `NGI-S` (as it is on production miarka in Stockholm), the following params are automatically set for the rnaseq pipeline when using `--genome GRCh38`: | ||
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| - `gencode = true` | ||
| - `save_reference = true` | ||
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| Usage with nf-core/rnaseq: | ||
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| ```bash | ||
| nextflow run nf-core/rnaseq -profile uppmax --genome GRCh38 ... | ||
| ``` | ||
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| ### Params files | ||
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| Params files are available in `conf/pipeline/rnaseq/uppmax/`, both containing GRCh38 genome reference paths for bed12 and gtf files. They overwrite the genomes map from igenomes from GRCh38 and can then be used even when the `--genome` parameter is set to a different genome. | ||
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| `rnaseq_sthlm_params.yaml` only contains the genome reference paths, so optional when not using GRCh38. | ||
| `rnaseq_sthlm_Pico-V3-trim_UMIs_params.yaml` is mandatory for Pico V3 library prep with UMIs, when using or not using the `--genome GRCh38` parameter. | ||
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| Example: | ||
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| ```bash | ||
| nextflow run nf-core/rnaseq -profile uppmax --genome GRCh38 \ | ||
| -params-file conf/pipeline/rnaseq/uppmax/rnaseq_sthlm_Pico-V3-trim_UMIs_params.yaml | ||
| ``` |
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