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Copy file name to clipboardexpand all lines: docs/src/guides/update-workflow.rst
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- `curated metadata including latitudes/longitudes, clade annotations, and low quality sequences <https://github.com/nextstrain/ncov/commits/master>`__
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- bug fixes
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- `new features <../reference/change_log>`__
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- :doc:`new features <../reference/change_log>`
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Update your local copy of the workflow, to benefit from these changes.
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# and then replay your local changes on top of those incoming changes.
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git pull --rebase origin master
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Alternately, download a specific version of the workflow that you know works for you. We create new `releases of the workflow <https://github.com/nextstrain/ncov/releases/>`__ any time we introduce breaking changes, so you can choose when to update based on `what has changed <../reference/change_log>`__.
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Alternately, download a specific version of the workflow that you know works for you. We create new `releases of the workflow <https://github.com/nextstrain/ncov/releases/>`__ any time we introduce breaking changes, so you can choose when to update based on :doc:`what has changed <../reference/change_log>`.
Copy file name to clipboardexpand all lines: docs/src/reference/change_log.md
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## New features since last version update
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- 11 August 2021: Add support for "Sequences" and "Patient status metadata" downloads from GISAID's search interface including [documentation in the tutorial of how to use these data](../guides/data-prep.html#curate-data-from-gisaid-search-and-downloads). ([#701](https://github.com/nextstrain/ncov/pull/701))
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- 11 August 2021: Add support for "Sequences" and "Patient status metadata" downloads from GISAID's search interface including [documentation in the tutorial of how to use these data](https://docs.nextstrain.org/projects/ncov/en/latest/guides/data-prep/gisaid-search.html). ([#701](https://github.com/nextstrain/ncov/pull/701))
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- 6 August 2021: We've replaced the mechanisms that support remote file inputs (e.g. `s3://` URLs) to improve internal workflow structure, extend support to `gs://`, `http://`, and `https://` URLs, and expand support for compressed inputs.
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Our [remote file inputs documentation](remote_inputs) is updated to reflect the changes.
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## v7 (27 May 2021)
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For more details about this release, see [the configuration reference for the new "sanitize metadata" parameters](configuration.html#sanitize_metadata) and [the corresponding pull request](https://github.com/nextstrain/ncov/pull/640).
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For more details about this release, see [the configuration reference for the new "sanitize metadata" parameters](https://docs.nextstrain.org/projects/ncov/en/latest/reference/workflow-config-file.html#sanitize-metadata) and [the corresponding pull request](https://github.com/nextstrain/ncov/pull/640).
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### Major changes
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## New features since last version update
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- 25 May 2021: Support custom Auspice JSON prefixes with a new configuration parameter, `auspice_json_prefix`. [See the configuration reference for more details](configuration.html#auspice_json_prefix). ([#643](https://github.com/nextstrain/ncov/pull/643))
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- 25 May 2021: Support custom Auspice JSON prefixes with a new configuration parameter, `auspice_json_prefix`. [See the configuration reference for more details](https://docs.nextstrain.org/projects/ncov/en/latest/reference/workflow-config-file.html#auspice-json-prefix). ([#643](https://github.com/nextstrain/ncov/pull/643))
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## v6 (20 May 2021)
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### Major changes
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- Drop support for old sequence/metadata inputs. This change removes support for the `config["sequences"]` and `config["metadata"]` starting points for the workflow in favor of the more flexible [`config["inputs"]` format](configuration.html#inputs).
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- Drop support for old sequence/metadata inputs. This change removes support for the `config["sequences"]` and `config["metadata"]` starting points for the workflow in favor of the more flexible [`config["inputs"]` format](https://docs.nextstrain.org/projects/ncov/en/latest/reference/workflow-config-file.html#inputs).
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- Use `nextalign` for alignment instead of `mafft`. This change completely removes support for `mafft` in favor of `nextalign`. Future versions may reinstate `mafft` support as part of `augur align` updates.
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### Minor changes
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## New features since last version update
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- 20 April 2021: Surface emerging lineage as a colorby. This replaces the rather stale color by "Emerging Clade" with a new color by "Emerging Lineage". This focuses on PANGO lineages that are of interest triangulated by [CoVariants](https://covariants.org/), [PANGO](https://cov-lineages.org/) international lineage reports, [CDC](https://www.cdc.gov/coronavirus/2019-ncov/cases-updates/variant-surveillance/variant-info.html) VUIs and VOCs and [PHE](https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/975742/Variants_of_Concern_VOC_Technical_Briefing_8_England.pdf) VUIs and VOCs. The intention is for the listing at `emerging_lineages.tsv` to be updated frequently with new lineages added and no longer interesting lineages dropped. [#609](https://github.com/nextstrain/ncov/pull/609)
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- 20 April 2021: Surface emerging lineage as a colorby. This replaces the rather stale color by "Emerging Clade" with a new color by "Emerging Lineage". This focuses on PANGO lineages that are of interest triangulated by [CoVariants](https://covariants.org/), [PANGO](https://cov-lineages.org/) international lineage reports, [CDC](https://www.cdc.gov/covid/php/variants/index.html) VUIs and VOCs and [PHE](https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/975742/Variants_of_Concern_VOC_Technical_Briefing_8_England.pdf) VUIs and VOCs. The intention is for the listing at `emerging_lineages.tsv` to be updated frequently with new lineages added and no longer interesting lineages dropped. [#609](https://github.com/nextstrain/ncov/pull/609)
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- 12 April 2021: Calculate current clade frequency and logistic growth rate across nodes in the phylogeny. This produces a new `logistic_growth.json` file and uses this file to add a coloring the final Auspice JSON. Implementation choices are discussed in PR [#595](https://github.com/nextstrain/ncov/pull/595).
Copy file name to clipboardexpand all lines: docs/src/reference/remote_inputs.rst
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@@ -27,7 +27,7 @@ Entire metadata & sequences data is uploaded from the ``ncov-ingest`` workflows
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- ``metadata.tsv.zst`` and ``metadata.tsv.gz``
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- ``sequences.fasta.zst`` and ``sequences.fasta.xz``
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- ``nextclade.tsv.zst`` and ``nextclade.tsv.gz``
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- ``aligned.fasta.zst`` and ``aligned.fasta.xz`` (Alignment via `Nextclade <https://docs.nextstrain.org/projects/nextclade/en/stable/user/output-files.html#aligned-nucleotide-sequences>`__. The default reference genome is `MN908947 <https://www.ncbi.nlm.nih.gov/nuccore/MN908947>`__ (Wuhan-Hu-1))
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- ``aligned.fasta.zst`` and ``aligned.fasta.xz`` (Alignment via `Nextclade <https://docs.nextstrain.org/projects/nextclade/en/stable/user/output-files/02-nuc-alignment.html>`__. The default reference genome is `MN908947 <https://www.ncbi.nlm.nih.gov/nuccore/MN908947>`__ (Wuhan-Hu-1))
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- ``additional_info.tsv.zst`` and ``additional_info.tsv.gz`` (GISAID only)
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- ``flagged_metadata.txt.zst`` and ``flagged_metadata.txt.gz`` (GISAID only)
Copy file name to clipboardexpand all lines: docs/src/reference/workflow-config-file.rst
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Parameters in this section define the main inputs and outputs of the workflow, as well as the commonly used ``subsampling`` rule.
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Often these will be the only parameters you need to modify.
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.. _inputs:
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inputs
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------
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-----------
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- type: object
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- description: Schemes for subsampling data prior to phylogenetic inference to avoid sampling bias or focus an analysis on specific spatial and/or temporal scales. `See the SARS-CoV-2 tutorial for more details on defining subsampling schemes <../reference/customizing-analysis.html#subsampling>`__.
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- description: Schemes for subsampling data prior to phylogenetic inference to avoid sampling bias or focus an analysis on specific spatial and/or temporal scales. See the :doc:`genomic surveillance tutorial <../tutorial/genomic-surveillance>` for an example.
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Predefined subsampling schemes are:
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- description: A list of prefixes to strip from strain names in metadata and sequence records to maintain consistent strain names when analyzing data from multiple sources.
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- default: ``["hCoV-19/", "SARS-CoV-2/"]``
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.. _auspice_json_prefix:
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auspice_json_prefix
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-------------------
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Each top-level parameter here corresponds to a single Snakemake rule.
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Note that ``subsampling`` is a commonly used rule configuration which is described separately in the Primary configuration section.
Copy file name to clipboardexpand all lines: docs/src/tutorial/custom-data.rst
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-------------
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1. :doc:`example-data`. This tutorial sets up the command line environment used in the following tutorial.
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2. `Register for a GISAID account <https://www.gisaid.org/registration/register/>`_, if you do not have one yet. However, registration may take a few days. Follow :doc:`alternative data preparation methods <../guides/data-prep/index>` in place of :ref:`custom-data-curate-data-from-gisaid`, if you wish to continue the following tutorial in the meantime.
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2. `Register for a GISAID account <https://gisaid.org/register//>`_, if you do not have one yet. However, registration may take a few days. Follow :doc:`alternative data preparation methods <../guides/data-prep/index>` in place of :ref:`custom-data-curate-data-from-gisaid`, if you wish to continue the following tutorial in the meantime.
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Setup
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We will retrieve 10 sequences from GISAID's EpiCoV database.
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1. Navigate to `GISAID <https://www.gisaid.org/>`__ and select **Login**.
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1. Navigate to `GISAID <https://gisaid.org/>`__ and select **Login**.
Copy file name to clipboardexpand all lines: docs/src/tutorial/genomic-surveillance.rst
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-------------
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1. :doc:`custom-data`. This tutorial introduces concepts expanded by the following tutorial.
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2. `Register for a GISAID account <https://www.gisaid.org/registration/register/>`_, if you do not have one yet. However, registration may take a few days. Follow :doc:`alternative data preparation methods <../guides/data-prep/index>` in place of :ref:`genomic-surveillance-curate-data-from-gisaid`, if you wish to continue the following tutorial in the meantime.
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2. `Register for a GISAID account <https://gisaid.org/registration/>`_, if you do not have one yet. However, registration may take a few days. Follow :doc:`alternative data preparation methods <../guides/data-prep/index>` in place of :ref:`genomic-surveillance-curate-data-from-gisaid`, if you wish to continue the following tutorial in the meantime.
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We will download a focal set of Idaho sequences from GISAID's EpiCoV database.
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1. Navigate to `GISAID <https://www.gisaid.org/>`__, **Login**, and go to **EpiCoV** > **Search**.
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1. Navigate to `GISAID <https://gisaid.org/>`__, **Login**, and go to **EpiCoV** > **Search**.
Copy file name to clipboardexpand all lines: docs/src/tutorial/next-steps.rst
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- `An applied genomic epidemiological handbook <https://alliblk.github.io/genepi-book/intro.html>`__ by Allison Black and Gytis Dudas
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- `Genomic Epidemiology Seminar Series <https://czgenepi.org/resources>`__ by Chan Zuckerberg Initiative Genomic Epidemiology (CZ GEN EPI)
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- `COVID-19 Genomic Epidemiology Toolkit <https://www.cdc.gov/amd/training/covid-19-gen-epi-toolkit.html>`__ by Centers for Disease Control and Prevention (CDC)
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- `COVID-19 Genomic Epidemiology Toolkit <https://www.cdc.gov/advanced-molecular-detection/php/training/?CDC_AAref_Val=https://www.cdc.gov/amd/training/covid-19-gen-epi-toolkit.html>`__ by Centers for Disease Control and Prevention (CDC)
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- :doc:`Review all possible options to configure your SARS-CoV-2 analyses with Nextstrain <../reference/workflow-config-file>`.
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- Watch `this 1-hour video overview <https://youtu.be/m4_F2tG58Pc>`__ by Heather Blankenship on how to deploy Nextstrain for a Public Health lab.
Copy file name to clipboardexpand all lines: docs/src/visualization/interpretation.rst
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- How to interact with Auspice (the engine for viewing trees): https://neherlab.org/201901_krisp_auspice.html
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- Overview of genomic epidemiology (older, but still relevant and clear): http://evolve.zoo.ox.ac.uk/Evolve/Oliver_Pybus_files/EvolAnalysisOfDynamicsOfViruses.pdf
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- Overview of genomic epidemiology (older, but still relevant and clear): https://www.nature.com/articles/nrg2583
Copy file name to clipboardexpand all lines: docs/src/visualization/narratives.rst
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For examples, `see our weekly Situation Reports <https://nextstrain.org/ncov-sit-reps>`__ from the first several months of the pandemic.
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You can `read more about narratives <https://nextstrain.org/docs/narratives/introduction>`__ or `watch our Nextstrain narratives tutorial videos <https://www.youtube.com/playlist?list=PLsFWZl6SQqWxN9SkbgdjU8sylIfhZNDiW>`_. We've also `provided a template narrative file <https://github.com/nextstrain/ncov/tree/master/narratives/ncov_template_narrative.md>`__ for you to edit.
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You can `read more about narratives <https://nextstrain.org/docs/narratives/introduction>`__ or `watch our Nextstrain narratives tutorial videos <https://www.youtube.com/playlist?list=PLsFWZl6SQqWxN9SkbgdjU8sylIfhZNDiW>`_. We've also `provided a template narrative file <https://github.com/nextstrain/ncov/blob/-/narratives/ncov_template_narrative.md>`__ for you to edit.
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You can preview the template narrative by navigating to https://nextstrain.org/community/narratives/nextstrain/ncov/template/narrative.
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