Merge pull request artic-network#75 from joshquick/master

Update pooling volumes for v4
leocaserta · Jan 7, 2022 · 6c26f71 · 6c26f71
2 parents b3f2dda + a937e90
commit 6c26f71
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diff --git a/primer_schemes/nCoV-2019/V4.1/README b/primer_schemes/nCoV-2019/V4.1/README
@@ -0,0 +1,37 @@
+This primer scheme is an update to the V4 scheme which restores the funtionality of some primers against the Omicron variant. The following changes are made;
+
+Added to pool 1:
+SARS-CoV-2_23_RIGHT_alt1
+SARS-CoV-2_27_RIGHT_alt1
+SARS-CoV-2_79_RIGHT_alt1
+SARS-CoV-2_89_LEFT_alt1
+SARS-CoV-2_89_RIGHT_alt1
+
+Added to pool 2:
+SARS-CoV-2_10_LEFT_alt1
+SARS-CoV-2_10_RIGHT_alt1
+SARS-CoV-2_76_LEFT_alt1
+SARS-CoV-2_76_RIGHT_alt1
+SARS-CoV-2_88_LEFT_alt1
+SARS-CoV-2_90_RIGHT_alt1
+
+Guide to pooling volumes are;
+
+2x volume:
+SARS-CoV-2_1_LEFT & SARS-CoV-2_1_RIGHT
+SARS-CoV-2_7_LEFT & SARS-CoV-2_7_RIGHT
+SARS-CoV-2_13_LEFT & SARS-CoV-2_13_RIGHT
+SARS-CoV-2_17_LEFT & SARS-CoV-2_17_RIGHT
+SARS-CoV-2_27_LEFT & SARS-CoV-2_27_RIGHT
+SARS-CoV-2_45_LEFT & SARS-CoV-2_45_RIGHT
+SARS-CoV-2_59_LEFT & SARS-CoV-2_59_RIGHT
+SARS-CoV-2_60_LEFT & SARS-CoV-2_60_RIGHT
+SARS-CoV-2_61_LEFT & SARS-CoV-2_61_RIGHT
+SARS-CoV-2_64_LEFT & SARS-CoV-2_64_RIGHT
+SARS-CoV-2_79_LEFT & SARS-CoV-2_79_RIGHT
+SARS-CoV-2_90_LEFT & SARS-CoV-2_90_RIGHT
+SARS-CoV-2_91_LEFT & SARS-CoV-2_91_RIGHT
+
+1x volume:
+All other primers including alts.
+
diff --git a/primer_schemes/nCoV-2019/V4.1/SARS-CoV-2.insert.bed b/primer_schemes/nCoV-2019/V4.1/SARS-CoV-2.insert.bed
@@ -0,0 +1,99 @@
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