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title: 使用Kraken进行16S/ITS物种注释(超快) | ||
date: '2025-01-14' | ||
linkTitle: /p/kraken-16s-its/ | ||
source: Asa's blog | ||
description: |- | ||
<img src="/p/kraken-16s-its/images/k16s1.png" alt="Featured image of post 使用Kraken进行16S/ITS物种注释(超快)" /><h2 id="introduction">Introduction</h2> | ||
<p>上次记录了一下qiime2的<a class="link" href="../amplicon-workflow" >扩增子分析流程</a>。但是实际使用时,在最后一步物种注释时卡住了,如果使用常用的<code>feature-classifier</code>方法,我尝试使用unite数据库鉴定了100条ITS序列,居然用了半个小时!尴尬的是,我有整整10万条ASV序列,这得跑到猴年马月。我想到做宏基因组时,千万级数量的reads用kraken做物种注释也不需要这么久,肯定有快的方法的。</p> | ||
<p>然后发现Kraken用于16S已经有文章了: | ||
Lu, J., Salzberg, S.L. Ultrafast and accurate 16S rRNA microbial community analysis using Kraken 2. Microbiome 8, 124 (2020). <a class="link" ... | ||
disable_comments: true | ||
--- | ||
<img src="/p/kraken-16s-its/images/k16s1.png" alt="Featured image of post 使用Kraken进行16S/ITS物种注释(超快)" /><h2 id="introduction">Introduction</h2> | ||
<p>上次记录了一下qiime2的<a class="link" href="../amplicon-workflow" >扩增子分析流程</a>。但是实际使用时,在最后一步物种注释时卡住了,如果使用常用的<code>feature-classifier</code>方法,我尝试使用unite数据库鉴定了100条ITS序列,居然用了半个小时!尴尬的是,我有整整10万条ASV序列,这得跑到猴年马月。我想到做宏基因组时,千万级数量的reads用kraken做物种注释也不需要这么久,肯定有快的方法的。</p> | ||
<p>然后发现Kraken用于16S已经有文章了: | ||
Lu, J., Salzberg, S.L. Ultrafast and accurate 16S rRNA microbial community analysis using Kraken 2. Microbiome 8, 124 (2020). <a class="link" ... |
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content/post/dirk-eddelbuettel-com-blog-2025-01-14-rprotobuf-0-4-23.md
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--- | ||
title: 'RProtoBuf 0.4.23 on CRAN: Mulitple Updates' | ||
date: '2025-01-14' | ||
linkTitle: http://dirk.eddelbuettel.com/blog/2025/01/14#rprotobuf_0.4.23 | ||
source: 'Thinking inside the box ' | ||
description: |2- | ||
<p>A new maintenance release 0.4.23 of <a | ||
href="https://dirk.eddelbuettel.com/code/rprotobuf.html">RProtoBuf</a> | ||
arrived on <a href="https://cran.r-project.org">CRAN</a> earlier today, | ||
about one year after the <a | ||
href="https://dirk.eddelbuettel.com/blog/2024/01/21#rprotobuf_0.4.22">previous | ||
update</a>. <a | ||
href="https://dirk.eddelbuettel.com/code/rprotobuf.html">RProtoBuf</a> | ||
provides <a href="https://www.r-project.org">R</a> with bindings for the | ||
<a href="https://github.com/google/protobuf">Google Protocol Buffers | ||
(“ProtoBuf”)</a> data encoding and serialization library used and | ||
released by Google, and ... | ||
disable_comments: true | ||
--- | ||
<p>A new maintenance release 0.4.23 of <a | ||
href="https://dirk.eddelbuettel.com/code/rprotobuf.html">RProtoBuf</a> | ||
arrived on <a href="https://cran.r-project.org">CRAN</a> earlier today, | ||
about one year after the <a | ||
href="https://dirk.eddelbuettel.com/blog/2024/01/21#rprotobuf_0.4.22">previous | ||
update</a>. <a | ||
href="https://dirk.eddelbuettel.com/code/rprotobuf.html">RProtoBuf</a> | ||
provides <a href="https://www.r-project.org">R</a> with bindings for the | ||
<a href="https://github.com/google/protobuf">Google Protocol Buffers | ||
(“ProtoBuf”)</a> data encoding and serialization library used and | ||
released by Google, and ... |
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...lowingdata-com-2025-01-14-distribution-of-days-that-were-hotter-than-average.md
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--- | ||
title: Distribution of days that were hotter than average | ||
date: '2025-01-14' | ||
linkTitle: https://flowingdata.com/2025/01/14/distribution-of-days-that-were-hotter-than-average/ | ||
source: FlowingData | ||
description: <p><a href="https://flowingdata.com/2025/01/14/distribution-of-days-that-were-hotter-than-average/"><img | ||
src="https://flowingdata.com/wp-content/uploads/2025/01/hotter-days-bbc-750x1414.png" | ||
style="max-width:100%;height:auto" /></a></p>BBC went with the gradient-filled frequency | ||
trails to show the distribution of daily…<p><strong>Tags:</strong> <a href="https://flowingdata.com/tag/bbc/" | ||
rel="tag">BBC</a>, <a href="https://flowingdata.com/tag/climate-change/" rel="tag">climate | ||
change</a>, <a href="https://flowingdata.com/tag/global-warming/" rel="tag">global | ||
... | ||
disable_comments: true | ||
--- | ||
<p><a href="https://flowingdata.com/2025/01/14/distribution-of-days-that-were-hotter-than-average/"><img src="https://flowingdata.com/wp-content/uploads/2025/01/hotter-days-bbc-750x1414.png" style="max-width:100%;height:auto" /></a></p>BBC went with the gradient-filled frequency trails to show the distribution of daily…<p><strong>Tags:</strong> <a href="https://flowingdata.com/tag/bbc/" rel="tag">BBC</a>, <a href="https://flowingdata.com/tag/climate-change/" rel="tag">climate change</a>, <a href="https://flowingdata.com/tag/global-warming/" rel="tag">global ... |
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content/post/flowingdata-com-2025-01-14-earth-was-hotter-than-ever-again.md
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--- | ||
title: Earth was hotter than ever, again | ||
date: '2025-01-14' | ||
linkTitle: https://flowingdata.com/2025/01/14/earth-was-hotter-than-ever-again/ | ||
source: FlowingData | ||
description: <p><a href="https://flowingdata.com/2025/01/14/earth-was-hotter-than-ever-again/"><img | ||
src="https://flowingdata.com/wp-content/uploads/2025/01/warning-threshold-NYT-750x461.png" | ||
style="max-width:100%;height:auto" /></a></p>This is some good step charting by | ||
Mira Rojanasakul for the New York…<p><strong>Tags:</strong> <a href="https://flowingdata.com/tag/climate-change/" | ||
rel="tag">climate change</a>, <a href="https://flowingdata.com/tag/global-warming/" | ||
rel="tag">global warming</a>, <a href="https://flowingdata.com/tag/new-york-times/" | ||
rel="tag">New York ... | ||
disable_comments: true | ||
--- | ||
<p><a href="https://flowingdata.com/2025/01/14/earth-was-hotter-than-ever-again/"><img src="https://flowingdata.com/wp-content/uploads/2025/01/warning-threshold-NYT-750x461.png" style="max-width:100%;height:auto" /></a></p>This is some good step charting by Mira Rojanasakul for the New York…<p><strong>Tags:</strong> <a href="https://flowingdata.com/tag/climate-change/" rel="tag">climate change</a>, <a href="https://flowingdata.com/tag/global-warming/" rel="tag">global warming</a>, <a href="https://flowingdata.com/tag/new-york-times/" rel="tag">New York ... |
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...bia-edu-2025-01-14-genre-fiction-some-genres-are-cumulative-and-some-are-not.md
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--- | ||
title: 'Genre fiction: Some genres are cumulative and some are not.' | ||
date: '2025-01-14' | ||
linkTitle: https://statmodeling.stat.columbia.edu/2025/01/14/genre-fiction-some-genres-are-cumulative-and-some-are-not/ | ||
source: Statistical Modeling, Causal Inference, and Social Science | ||
description: I’ve been reading some books about the history of twentieth-century | ||
mystery novels and science fiction stories, and one thing that struck me was that | ||
each of these genres had a sense of continuity. If you used a gimmick in a … | ||
<a href="https://statmodeling.stat.columbia.edu/2025/01/14/genre-fiction-some-genres-are-cumulative-and-some-are-not/">Continue | ||
reading <span class="meta-nav">→</span></a> ... | ||
disable_comments: true | ||
--- | ||
I’ve been reading some books about the history of twentieth-century mystery novels and science fiction stories, and one thing that struck me was that each of these genres had a sense of continuity. If you used a gimmick in a … <a href="https://statmodeling.stat.columbia.edu/2025/01/14/genre-fiction-some-genres-are-cumulative-and-some-are-not/">Continue reading <span class="meta-nav">→</span></a> ... |
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...gers-com-2025-01-basic-data-structures-in-r-vectors-matrices-and-data-frames.md
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--- | ||
title: 'Basic Data Structures in R: Vectors, Matrices, and Data Frames' | ||
date: '2025-01-14' | ||
linkTitle: https://www.r-bloggers.com/2025/01/basic-data-structures-in-r-vectors-matrices-and-data-frames/ | ||
source: R-bloggers | ||
description: |- | ||
<div style = "width:60%; display: inline-block; float:left; "> IntroductionIn the world of data analysis and statistical computing, R stands out as a powerful and versatile language. Its ability to handle complex data operations with ease makes it a favorite among data scientists, statisticians, and researchers. A...</div> | ||
<div style = "width: 40%; display: inline-block; float:right;"></div> | ||
<div style="clear: both;"></div> | ||
<strong>Continue reading</strong>: <a href="https://www.r-bloggers.com/2025/01/basic-data-structures-in-r-vectors-matrices-and-data-frames/">Basic Data Structures in R: ... | ||
disable_comments: true | ||
--- | ||
<div style = "width:60%; display: inline-block; float:left; "> IntroductionIn the world of data analysis and statistical computing, R stands out as a powerful and versatile language. Its ability to handle complex data operations with ease makes it a favorite among data scientists, statisticians, and researchers. A...</div> | ||
<div style = "width: 40%; display: inline-block; float:right;"></div> | ||
<div style="clear: both;"></div> | ||
<strong>Continue reading</strong>: <a href="https://www.r-bloggers.com/2025/01/basic-data-structures-in-r-vectors-matrices-and-data-frames/">Basic Data Structures in R: ... |
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...t/www-r-bloggers-com-2025-01-understanding-machine-learning-and-data-science.md
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--- | ||
title: Understanding Machine Learning and Data Science | ||
date: '2025-01-14' | ||
linkTitle: https://www.r-bloggers.com/2025/01/understanding-machine-learning-and-data-science/ | ||
source: R-bloggers | ||
description: |- | ||
<p>The post Understanding Machine Learning and Data Science appeared first on Data Science Tutorials<br /> | ||
Unravel the Future: Dive Deep into the World of Data Science Today! Data Science Tutorials.<br /> | ||
Understanding Machine Learning and Data Science, In today’s digital age, the terms “Machine Learning” and “Data Science” have become buzzwords, ...</p> | ||
<strong>Continue reading</strong>: <a href="https://www.r-bloggers.com/2025/01/understanding-machine-learning-and-data-science/">Understanding Machine Learning and Data ... | ||
disable_comments: true | ||
--- | ||
<p>The post Understanding Machine Learning and Data Science appeared first on Data Science Tutorials<br /> | ||
Unravel the Future: Dive Deep into the World of Data Science Today! Data Science Tutorials.<br /> | ||
Understanding Machine Learning and Data Science, In today’s digital age, the terms “Machine Learning” and “Data Science” have become buzzwords, ...</p> | ||
<strong>Continue reading</strong>: <a href="https://www.r-bloggers.com/2025/01/understanding-machine-learning-and-data-science/">Understanding Machine Learning and Data ... |