BHA4 Akio Ohta

• Who am I?
• projects
  • 1. Folding test tube rack (homework)
  • 2. the personal project
    • Sakura Zensen (Cherry blossom front)
    • What I want to do
    • Issues I have to consider now
  • 3. What I did as my personal project
    • Purpose
    • Method
    • Experiments & Results
      • Why agar melted by ultrasonic wave?
      • Moss plants experiments
    • BTW
  • Webcam Spectrophotometer

Who am I?

• Software engineer working on an internet advertising company.
• Like Python, and use also C++, JavaScript, OpenSCAD.
• https://github.com/Drunkar

projects

1. Folding test tube rack (homework)

• Test tube rack which can be made by folding it like origami from an a4 size clear file folder.
• https://github.com/Drunkar/folding_test_tube_rack

2. the personal project

mid-term presentation: https://youtu.be/lHk0ZrmN1bQ

As my personal project, I'd like to map a natural phenomenon to microorganisms. The phenomenon called as "Sakura Zensen" in Japanese. This is a weather term. In English, it seems to be called "Cherry blossom front".

Sakura Zensen (Cherry blossom front)

Many cherry trees are planted in Japan, but actually most of them are clones. So, with specific conditions such as high temperature, they all bloom at once.

In Japan, it gradually warms from the south, so the cherry blossoms bloom little by little from the south. It looks like a front line. This is why it's called Cherry Blossom Front.

When cherry blossoms bloom, Japanese people feel that "Spring has come". Sakura Zensen shows us the data "the arrival of spring" which is usually difficult to quantify.

What I want to do

Sakura Zensen is a data. It converted from a real world. In other words, atom to bit. It is definitely biosensing.

In my project, I will return this data to the world of atom again. But it's not to the macro world, to the micro world.

I will cultivate microorganisms in a medium in the shape of Japan. Breeding areas are limited to the shape where cherry blossoms have been already in bloom. The area where spring does not come yet will be sterilized. I am planning to use ultrasonic waves or ultraviolet rays for sterilization.

Issues I have to consider now

3. What I did as my personal project

Purpose

• To control microorganisms cultivation area

Method

• Some sterilization methods

Experiments & Results

video: https://youtu.be/SKyFr61yzyg

• Ultrasonic wave:

  • Buy and build a parametric speaker kit ( https://www.google.co.jp/webhp?sourceid=chrome-instant&rlz=1C5CHFA_enJP700JP700&ion=1&espv=2&ie=UTF-8#q=%E3%83%91%E3%83%A9%E3%83%A1%E3%83%88%E3%83%AA%E3%83%83%E3%82%AF%E3%82%B9%E3%83%94%E3%83%BC%E3%82%AB%E3%83%BC%E3%82%AD%E3%83%83%E3%83%88&* ).
  • Play white noise sound file from raspberry pi with pygame package.

• Ultraviolet:

  • Connect 3.3v out and GND of arduino to Ultraviolet LEDs ( http://akizukidenshi.com/catalog/g/gI-04775/ )

weapon	organism	cultivation	attack	results
Ultrasonic wave	bacteria	5 days	3 days	agar melted
Ultraviolet LED	bacteria	5 days	3 days	no change
Ultrasonic wave	moss	-	5 days	no change

Why agar melted by ultrasonic wave?

• Agar got heated by vibration caused by ultrasonic waves.
  • Heat-resistant medium is needed.
  • Higher accuracy control of ultrasonic position.

Moss plants experiments

Attack by ultrasonic wave, but change.

• day 1

• day 5

BTW

Cherry blossoms are starting to bloom... (04 Apr 2017)

Webcam Spectrophotometer

I built BHA Webcam Spectrometer( https://github.com/BioHackAcademy/BHA_Webcam_Spectrophotometer ) and run web app with docker on AWS ec2.

However, he has not have his turn yet in my project. :-(