Hi @timflutre
Hope you are fine and keeping safe
I am running kneaddata, but it gave and error with Trimmomatic
**kneaddata –input1 /media/sf_Shared_folder/ovas/Final/81_R1.fastq --input2 /media/sf_Shared_folder/ovas/Final/81_R2.fastq --reference-db /home/qiime2/ref_dtabase/ --output kneaddata_81 --trimmomatic /home/qiime2/Trimmomatic-0.33
Reformatting file sequence identifiers …
Reformatting file sequence identifiers …
Reordering read identifiers …
Initial number of reads ( /home/qiime2/kneaddata_81/reordered_qhik9bbx_reformatted_identifierse3uxy95f_81_R1 ): 22625162.0
Initial number of reads ( /home/qiime2/kneaddata_81/reordered_enctz3x3_reformatted_identifiersd8ic66o3_81_R2 ): 22625162.0
Running Trimmomatic …
CRITICAL ERROR: Error executing: java -Xmx500m -jar /home/qiime2/Trimmomatic-0.33/trimmomatic-0.33.jar PE -threads 1 -phred33 /home/qiime2/kneaddata_81/reordered_qhik9bbx_reformatted_identifierse3uxy95f_81_R1 /home/qiime2/kneaddata_81/reordered_enctz3x3_reformatted_identifiersd8ic66o3_81_R2 /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.1.fastq /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.single.1.fastq /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.2.fastq /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.single.2.fastq MINLEN:60 ILLUMINACLIP:/home/qiime2/miniconda/envs/kneaddata/lib/python3.8/site-packages/kneaddata/adapters/NexteraPE-PE.fa:2:30:10:8:TRUE SLIDINGWINDOW:4:20 MINLEN:75
Error message returned from Trimmomatic :
<JAVA_HOME>/lib/ext exists, extensions mechanism no longer supported; Use -classpath instead.
.Error: Could not create the Java Virtual Machine.
Error: A fatal exception has occurred. Program will exit**
I posted not biobakery forum, but didn't got any response yet can understand the time constraints and busy schedule of every one there).
Kindly guide how to overcome this issue.
Hi @timflutre
Hope you are fine and keeping safe
I am running kneaddata, but it gave and error with Trimmomatic
**kneaddata –input1 /media/sf_Shared_folder/ovas/Final/81_R1.fastq --input2 /media/sf_Shared_folder/ovas/Final/81_R2.fastq --reference-db /home/qiime2/ref_dtabase/ --output kneaddata_81 --trimmomatic /home/qiime2/Trimmomatic-0.33
Reformatting file sequence identifiers …
Reformatting file sequence identifiers …
Reordering read identifiers …
Initial number of reads ( /home/qiime2/kneaddata_81/reordered_qhik9bbx_reformatted_identifierse3uxy95f_81_R1 ): 22625162.0
Initial number of reads ( /home/qiime2/kneaddata_81/reordered_enctz3x3_reformatted_identifiersd8ic66o3_81_R2 ): 22625162.0
Running Trimmomatic …
CRITICAL ERROR: Error executing: java -Xmx500m -jar /home/qiime2/Trimmomatic-0.33/trimmomatic-0.33.jar PE -threads 1 -phred33 /home/qiime2/kneaddata_81/reordered_qhik9bbx_reformatted_identifierse3uxy95f_81_R1 /home/qiime2/kneaddata_81/reordered_enctz3x3_reformatted_identifiersd8ic66o3_81_R2 /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.1.fastq /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.single.1.fastq /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.2.fastq /home/qiime2/kneaddata_81/81_R1_kneaddata.trimmed.single.2.fastq MINLEN:60 ILLUMINACLIP:/home/qiime2/miniconda/envs/kneaddata/lib/python3.8/site-packages/kneaddata/adapters/NexteraPE-PE.fa:2:30:10:8:TRUE SLIDINGWINDOW:4:20 MINLEN:75
Error message returned from Trimmomatic :
<JAVA_HOME>/lib/ext exists, extensions mechanism no longer supported; Use -classpath instead.
.Error: Could not create the Java Virtual Machine.
Error: A fatal exception has occurred. Program will exit**
I posted not biobakery forum, but didn't got any response yet can understand the time constraints and busy schedule of every one there).
Kindly guide how to overcome this issue.