diff --git a/TUnit.Assertions.Tests/Bugs/Tests5841.cs b/TUnit.Assertions.Tests/Bugs/Tests5841.cs
new file mode 100644
index 0000000000..ca0549b6df
--- /dev/null
+++ b/TUnit.Assertions.Tests/Bugs/Tests5841.cs
@@ -0,0 +1,39 @@
+namespace TUnit.Assertions.Tests.Bugs;
+
+/// <summary>
+/// Regression tests for issue #5841.
+/// IsEquivalentTo must not invoke property/field getters whose return type is a ref struct
+/// (e.g. ReadOnlySpan&lt;T&gt;, reachable via ReadOnlyMemory&lt;T&gt;.Span). Ref structs cannot
+/// be boxed, so RuntimeMethodInfo.Invoke throws NotSupportedException.
+/// </summary>
+public class Tests5841
+{
+    public class HasMemoryProperty
+    {
+        public ReadOnlyMemory<byte> Memory { get; init; }
+    }
+
+    public class HasSpanProperty
+    {
+        public ReadOnlySpan<byte> Span => default;
+        public int Value { get; init; }
+    }
+
+    [Test]
+    public async Task IsEquivalentTo_with_ReadOnlyMemory_property_does_not_invoke_Span_getter()
+    {
+        var a = new HasMemoryProperty { Memory = new byte[] { 1, 2, 3 } };
+        var b = new HasMemoryProperty { Memory = new byte[] { 1, 2, 3 } };
+
+        await Assert.That(a).IsEquivalentTo(b);
+    }
+
+    [Test]
+    public async Task IsEquivalentTo_skips_ref_struct_returning_property()
+    {
+        var a = new HasSpanProperty { Value = 7 };
+        var b = new HasSpanProperty { Value = 7 };
+
+        await Assert.That(a).IsEquivalentTo(b);
+    }
+}
diff --git a/TUnit.Assertions/Conditions/Helpers/ReflectionHelper.cs b/TUnit.Assertions/Conditions/Helpers/ReflectionHelper.cs
index 501523b5be..c9e932848d 100644
--- a/TUnit.Assertions/Conditions/Helpers/ReflectionHelper.cs
+++ b/TUnit.Assertions/Conditions/Helpers/ReflectionHelper.cs
@@ -35,19 +35,54 @@ private static MemberInfo[] BuildMembersToCompare(Type type)
         var members = new List<MemberInfo>();
 
         // Filter out indexed properties (properties with parameters like this[int index])
+        // and members whose type is a ref struct (IsByRefLike). Ref structs cannot be boxed,
+        // so PropertyInfo.GetValue / FieldInfo.GetValue throws NotSupportedException on them.
+        // Common offender: ReadOnlyMemory<T>.Span returns ReadOnlySpan<T>.
         var properties = type.GetProperties(BindingFlags.Public | BindingFlags.Instance);
         foreach (var prop in properties)
         {
-            if (prop.GetIndexParameters().Length == 0 && prop.CanRead && prop.GetMethod?.IsPublic == true)
+            if (prop.GetIndexParameters().Length == 0
+                && prop.CanRead
+                && prop.GetMethod?.IsPublic == true
+                && !IsByRefLike(prop.PropertyType))
             {
                 members.Add(prop);
             }
         }
 
-        members.AddRange(type.GetFields(BindingFlags.Public | BindingFlags.Instance));
+        foreach (var field in type.GetFields(BindingFlags.Public | BindingFlags.Instance))
+        {
+            if (!IsByRefLike(field.FieldType))
+            {
+                members.Add(field);
+            }
+        }
+
         return members.ToArray();
     }
 
+    private static bool IsByRefLike(Type type)
+    {
+#if NET5_0_OR_GREATER
+        return type.IsByRefLike;
+#else
+        if (!type.IsValueType)
+        {
+            return false;
+        }
+
+        foreach (var attr in type.GetCustomAttributesData())
+        {
+            if (attr.AttributeType.FullName == "System.Runtime.CompilerServices.IsByRefLikeAttribute")
+            {
+                return true;
+            }
+        }
+
+        return false;
+#endif
+    }
+
     /// <summary>
     /// Gets the value of a member (property or field) from an object.
     /// </summary>