diff --git a/README.md b/README.md index 773e4c5..00f5f4e 100644 --- a/README.md +++ b/README.md @@ -112,14 +112,14 @@ cd RNA-seek/ sinteractive --mem=110g --cpus-per-task=12 --gres=lscratch:200 module purge module load singularity snakemake -./rna-seek run --input .tests/*.R?.fastq.gz --output /scratch/$USER/LOCAL_RNA --genome hg38_30 --mode local +./rna-seek run --input .tests/*.R?.fastq.gz --output /data/$USER/RNA_hg38 --genome hg38_30 --mode local # @slurm: uses slurm and singularity execution method # The slurm MODE will submit jobs to the cluster. # It is recommended running rna-seek in this mode. module purge module load singularity snakemake -./rna-seek run --input .tests/*.R?.fastq.gz --output /scratch/$USER/LOCAL_RNA --genome hg38_30 --mode slurm +./rna-seek run --input .tests/*.R?.fastq.gz --output /data/$USER/RNA_hg38 --genome hg38_30 --mode slurm ``` ### 4. Contribute diff --git a/docs/RNA-seq/TLDR-RNA-seq.md b/docs/RNA-seq/TLDR-RNA-seq.md index f014910..7226246 100644 --- a/docs/RNA-seq/TLDR-RNA-seq.md +++ b/docs/RNA-seq/TLDR-RNA-seq.md @@ -78,14 +78,14 @@ module load singularity snakemake rna-seek build --ref-fa GRCh38.primary_assembly.genome.fa \ --ref-name hg38 \ --ref-gtf gencode.v36.primary_assembly.annotation.gtf \ - --gtf-ver 36 --output /scratch/$USER/hg38_36 --dry-run + --gtf-ver 36 --output /data/$USER/hg38_36 --dry-run # Build Step 5.) Submit the build pipeline to cluster rna-seek build --ref-fa GRCh38.primary_assembly.genome.fa \ --ref-name hg38 \ --ref-gtf gencode.v36.primary_assembly.annotation.gtf \ - --gtf-ver 36 --output /scratch/$USER/hg38_36 + --gtf-ver 36 --output /data/$USER/hg38_36 ``` An email notification will be sent out when the pipeline starts and ends. Once the build pipeline completes, you can run RNA-seek with the provided test dataset. Please see the intructions below for more information. @@ -114,8 +114,8 @@ module load singularity snakemake # Test data consists of sub sampled FastQ files rna-seek run \ --input RNA-seek/.tests/*.R?.fastq.gz \ - --output /scratch/${USER}/runner_hg38_36/ \ - --genome /scratch/${USER}/hg38_36/hg38_36.json \ + --output /data/${USER}/runner_hg38_36/ \ + --genome /data/${USER}/hg38_36/hg38_36.json \ --mode slurm \ --star-2-pass-basic \ --dry-run @@ -132,8 +132,8 @@ Kick off the pipeline by submiting the master job to the cluster. It is essentia # and with the test dataset rna-seek run \ --input RNA-seek/.tests/*.R?.fastq.gz \ - --output /scratch/${USER}/runner_hg38_36/ \ - --genome /scratch/${USER}/hg38_36/hg38_36.json \ + --output /data/${USER}/runner_hg38_36/ \ + --genome /data/${USER}/hg38_36/hg38_36.json \ --mode slurm \ --star-2-pass-basic \ --dry-run