diff --git a/README.md b/README.md index 6a52dae..9db3807 100644 --- a/README.md +++ b/README.md @@ -101,6 +101,16 @@ Run BLAZE in high-sensitivity mode: the expected number of cells is set to be 10 blaze --high-sensitivity-mode --expect-cells=1000 --threads=12 path/to/fastq_pass ``` +### Include ambient mRNA +If you run BLAZE with `--count-threshold 0` and no value for `--expect-cells`, it'll assign barcodes to the reads where it can find them and output these in matched_reads.fastq.gz regardless of the reads being considered true cells or ambient mRNA. + +## **Example code:** + +Run BLAZE in default mode: the expected number of cells is set to be 1000 and run with 12 threads +``` +blaze --count-threshold=0 --threads=12 path/to/fastq_pass +``` + ## Rerun blaze or update previous run(s) By default, BLAZE reuses existing files if they exist. For example, if you need to change some settings and rerun BLAZE after running: