diff --git a/src/python/pants/backend/url_handlers/s3/integration_test.py b/src/python/pants/backend/url_handlers/s3/integration_test.py
index 418e734845e..acb2174831d 100644
--- a/src/python/pants/backend/url_handlers/s3/integration_test.py
+++ b/src/python/pants/backend/url_handlers/s3/integration_test.py
@@ -55,6 +55,12 @@ def fake_resolver_creator(session):
             assert session is fake_session
             return SimpleNamespace(load_credentials=lambda: fake_creds)
 
+        # Replace the simple fake_session object with a SimpleNamespace that has get_config_variable
+        fake_session = SimpleNamespace(
+            get_config_variable=lambda name: "us-west-2" if name == "region" else None
+        )
+        botocore.session = SimpleNamespace(get_session=lambda: fake_session)
+
         def fake_creds_ctor(access_key, secret_key):
             assert access_key == fake_creds.access_key
             assert secret_key == fake_creds.secret_key
@@ -64,8 +70,10 @@ def fake_creds_ctor(access_key, secret_key):
             create_credential_resolver=fake_resolver_creator, Credentials=fake_creds_ctor
         )
 
-        def fake_auth_ctor(creds):
+        def fake_auth_ctor(creds, service_name, region_name):
             assert creds is fake_creds
+            assert service_name == "s3"
+            assert region_name in ["us-east-1", "us-west-2"]
 
             def add_auth(request):
                 request.url == expected_url
@@ -73,7 +81,7 @@ def add_auth(request):
 
             return SimpleNamespace(add_auth=add_auth)
 
-        botocore.auth = SimpleNamespace(HmacV1Auth=fake_auth_ctor)
+        botocore.auth = SimpleNamespace(SigV4Auth=fake_auth_ctor)
 
         monkeypatch.setitem(sys.modules, "botocore", botocore)
 
diff --git a/src/python/pants/backend/url_handlers/s3/register.py b/src/python/pants/backend/url_handlers/s3/register.py
index ec756e6ef27..66af9bdea30 100644
--- a/src/python/pants/backend/url_handlers/s3/register.py
+++ b/src/python/pants/backend/url_handlers/s3/register.py
@@ -18,7 +18,7 @@
 from pants.util.strutil import softwrap
 
 CONTENT_TYPE = "binary/octet-stream"
-
+EMPTY_SHA256_HASH = "e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855"
 
 logger = logging.getLogger(__name__)
 
@@ -26,6 +26,7 @@
 @dataclass(frozen=True)
 class AWSCredentials:
     creds: Any
+    default_region: str | None
 
 
 @rule
@@ -48,10 +49,11 @@ async def access_aws_credentials() -> AWSCredentials:
         )
         raise
 
-    session = session.get_session()
-    creds = credentials.create_credential_resolver(session).load_credentials()
+    boto_session = session.get_session()
+    creds = credentials.create_credential_resolver(boto_session).load_credentials()
+    default_region = boto_session.get_config_variable("region")
 
-    return AWSCredentials(creds)
+    return AWSCredentials(creds=creds, default_region=default_region)
 
 
 @dataclass(frozen=True)
@@ -69,36 +71,46 @@ async def download_from_s3(
 ) -> Digest:
     from botocore import auth, compat, exceptions  # pants: no-infer-dep
 
-    # NB: The URL for auth is expected to be in path-style
-    path_style_url = "https://s3"
+    virtual_hosted_url = f"https://{request.bucket}.s3.amazonaws.com/{request.key}"
     if request.region:
-        path_style_url += f".{request.region}"
-    path_style_url += f".amazonaws.com/{request.bucket}/{request.key}"
+        virtual_hosted_url = (
+            f"https://{request.bucket}.s3.{request.region}.amazonaws.com/{request.key}"
+        )
+
     if request.query:
-        path_style_url += f"?{request.query}"
+        virtual_hosted_url += f"?{request.query}"
 
     headers = compat.HTTPHeaders()
     http_request = SimpleNamespace(
-        url=path_style_url,
+        url=virtual_hosted_url,
         headers=headers,
         method="GET",
         auth_path=None,
+        data=None,
+        params={},
+        context={},
+        body={},
     )
     # NB: The added Auth header doesn't need to be valid when accessing a public bucket. When
     # hand-testing, you MUST test against a private bucket to ensure it works for private buckets too.
-    signer = auth.HmacV1Auth(aws_credentials.creds)
+    # adding x-amz-content-SHA256 as per boto code
+    # ref link - https://github.com/boto/botocore/blob/547b20801770c8ea4255ee9c3b809fea6b9f6bc4/botocore/auth.py#L52C1-L54C2
+    headers.add_header(
+        "X-Amz-Content-SHA256",
+        EMPTY_SHA256_HASH,
+    )
+
+    # We require a region to sign the request with sigv4
+    # If we don't know where the bucket is, default to the region from the credentials
+    # and fallback to us-east-1
+    signing_region = request.region or aws_credentials.default_region or "us-east-1"
+
+    signer = auth.SigV4Auth(aws_credentials.creds, "s3", signing_region)
     try:
         signer.add_auth(http_request)
     except exceptions.NoCredentialsError:
         pass  # The user can still access public S3 buckets without credentials
 
-    virtual_hosted_url = f"https://{request.bucket}.s3"
-    if request.region:
-        virtual_hosted_url += f".{request.region}"
-    virtual_hosted_url += f".amazonaws.com/{request.key}"
-    if request.query:
-        virtual_hosted_url += f"?{request.query}"
-
     return await Get(
         Digest,
         NativeDownloadFile(