ERROR ~ No such file or directory: https://mirbase.org/ftp/CURRENT/mature.fa.gz

[zyyy97]

Description of feature

Hello, It's a great job!
But when I used this pipeline, something went wrong.

nextflow run nf-core/smrnaseq -profile docker --input samplesheet.csv --genome 'GRCh37' --mirtrace_species 'hsa' --protocol 'illumina' --outdir z -r 2.0.0

!! Only displaying parameters that differ from the pipeline defaults !!

If you use nf-core/smrnaseq for your analysis please cite:

• The pipeline
  https://zenodo.org/badge/latestdoi/140590861

• The nf-core framework
  https://doi.org/10.1038/s41587-020-0439-x

• Software dependencies
  https://github.com/nf-core/smrnaseq/blob/master/CITATIONS.md

---

ERROR ~ No such file or directory: https://mirbase.org/ftp/CURRENT/mature.fa.gz

-- Check script '/home/zhouyu/.nextflow/assets/nf-core/smrnaseq/./workflows/smrnaseq.nf' at line: 54 or see '.nextflow.log' file for more details

how can deal with this issue? thank you

[kopichris]

Hi @zyyy97, I am not a maintainer, but happy to help troubleshoot. This error occurs because the FASTA file containing the mature RNA sequences are no longer available at: https://mirbase.org/ftp/CURRENT/mature.fa.gz, but are now available at: https://mirbase.org/download/mature.fa. Using an updated version of the pipeline should resolve your issue (version 2.4.0, for example).

[zyyy97]

Thank you very much！