From 6e2bd72db7328454020cdef157ad29be420237c4 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Jan 2026 18:29:29 -0800 Subject: [PATCH 1/6] minor optimizations of hic track and contact map view --- .../java/org/igv/bedpe/ContactMapView.java | 145 +- .../java/org/igv/bedpe/InteractionTrack.java | 4 +- .../java/org/igv/bedpe/NestedArcRenderer.java | 38 +- .../igv/bedpe/WrappedInteractionSource.java | 42 +- test/data/wig/test.bedgraph | 19990 ++++++++-------- 5 files changed, 10168 insertions(+), 10051 deletions(-) diff --git a/src/main/java/org/igv/bedpe/ContactMapView.java b/src/main/java/org/igv/bedpe/ContactMapView.java index cf6497c201..41d1fb2449 100644 --- a/src/main/java/org/igv/bedpe/ContactMapView.java +++ b/src/main/java/org/igv/bedpe/ContactMapView.java @@ -40,9 +40,16 @@ public class ContactMapView extends JPanel implements IGVEventObserver { private RulerPanel rulerPanel; // Reference to ruler panel for synchronized updates private JTextField maxField; private JSlider maxSlider; + private JLabel binSizeLabel; // Display for current bin size private double sliderMinValue = 0; private double sliderMaxValue = 100; + // Throttle fields for slider updates: ensure color-scale updates occur at most once every 100 ms + private final Object sliderThrottleLock = new Object(); + private volatile long lastSliderApplyTime = 0; + private javax.swing.Timer sliderThrottleTimer = null; + private volatile double pendingSliderValue = Double.NaN; + // Cached data for rendering private volatile List cachedRecords; private volatile boolean isLoading = false; @@ -100,6 +107,24 @@ public void mouseMoved(MouseEvent e) { int coordY = binY * binSize + binSize / 2; track.setMarkerBounds(new int[]{coordX, coordY}); + // Lookup a contact record for these bins (or the transpose) and update tooltip + if (cachedRecords != null) { + final int searchBin1 = binX; + final int searchBin2 = binY; + ContactRecord found = cachedRecords.stream() + .filter(r -> (r.bin1() == searchBin1 && r.bin2() == searchBin2) || + (r.bin1() == searchBin2 && r.bin2() == searchBin1)) + .findFirst() + .orElse(null); + if (found != null) { + setToolTipText(String.format("Counts: %.2f", found.counts())); + } else { + setToolTipText(null); + } + } else { + setToolTipText(null); + } + //String tooltip = String.format("Bin1: %d (Coord: %d), Bin2: %d (Coord: %d)", binX, coordX, binY, coordY); //setToolTipText(tooltip); } @@ -275,6 +300,11 @@ public void setReferenceFrame(ReferenceFrame newFrame) { this.frame = new ReferenceFrame(newFrame); binSize = newBinSize; + // Update bin size label display + if (binSizeLabel != null) { + binSizeLabel.setText(formatBinSize(binSize)); + } + // Calculate how many bins are needed to span the requested genomic range int genomicSpan = (int) (newFrame.getEnd() - newFrame.getOrigin()); int requestedNBins = (int) Math.ceil((double) genomicSpan / binSize); @@ -448,7 +478,7 @@ private Image renderMapFromCache() { if (dataMatchesCurrentView && colorScaleNeedsUpdate) { // We have fresh data and need to compute/update color scale from it // First, compute the percentile and update slider range - double upper = computePercentileOnly(cachedRecords, .5); + double upper = initializeColorScale(cachedRecords); if (colorScale == null) { // Create new color scale @@ -576,20 +606,25 @@ public void windowClosing(WindowEvent e) { * This panel can be extended with additional controls in the future. */ private JPanel createControlPanel() { - JPanel controlPanel = new JPanel(new FlowLayout(FlowLayout.LEFT)); + JPanel controlPanel = new JPanel(); + controlPanel.setLayout(new BoxLayout(controlPanel, BoxLayout.X_AXIS)); controlPanel.setBorder(BorderFactory.createEmptyBorder(5, 5, 5, 5)); // Label for max value JLabel maxLabel = new JLabel("Color Scale Max:"); + maxLabel.setAlignmentY(Component.CENTER_ALIGNMENT); controlPanel.add(maxLabel); + controlPanel.add(Box.createHorizontalStrut(5)); + // Slider for max value - instance variable for this view // Initial range 0-100, will be updated dynamically this.maxSlider = new JSlider(0, 100, 50); - this.maxSlider.setPreferredSize(new Dimension(200, 25)); + this.maxSlider.setPreferredSize(new Dimension(133, 25)); + this.maxSlider.setMaximumSize(new Dimension(133, 25)); + this.maxSlider.setAlignmentY(Component.CENTER_ALIGNMENT); this.maxSlider.setToolTipText(String.format("Range: %.2f - %.2f", sliderMinValue, sliderMaxValue)); this.maxSlider.addChangeListener(e -> { - if (!this.maxSlider.getValueIsAdjusting()) { // Map slider value (0-100) to actual range (sliderMinValue to sliderMaxValue) double value = sliderMinValue + (sliderMaxValue - sliderMinValue) * this.maxSlider.getValue() / 100.0; this.maxField.setText(String.format("%.2f", value)); @@ -598,19 +633,17 @@ private JPanel createControlPanel() { this.maxSlider.setToolTipText(String.format("Value: %.2f (Range: %.2f - %.2f)", value, sliderMinValue, sliderMaxValue)); - // Update the color scale - String colorScaleKey = getColorScaleCacheKey(); - ContinuousColorScale colorScale = this.colorScaleCache.get(colorScaleKey); - if (colorScale != null) { - colorScale.setPosEnd(value); - this.repaint(); - } - } + // Throttled update of the color scale to avoid excessive updates while dragging + applySliderValueThrottled(value); }); controlPanel.add(this.maxSlider); + controlPanel.add(Box.createHorizontalStrut(5)); + // Text field for max value - instance variable for this view - this.maxField = new JTextField("10", 10); + this.maxField = new JTextField("10", 6); + this.maxField.setMaximumSize(this.maxField.getPreferredSize()); + this.maxField.setAlignmentY(Component.CENTER_ALIGNMENT); this.maxField.addActionListener(e -> { try { double newMax = Double.parseDouble(this.maxField.getText().trim()); @@ -634,8 +667,11 @@ private JPanel createControlPanel() { }); controlPanel.add(this.maxField); + controlPanel.add(Box.createHorizontalStrut(5)); + // Apply button JButton applyButton = new JButton("Apply"); + applyButton.setAlignmentY(Component.CENTER_ALIGNMENT); applyButton.addActionListener(e -> { try { String colorScaleKey = getColorScaleCacheKey(); @@ -660,6 +696,14 @@ private JPanel createControlPanel() { }); controlPanel.add(applyButton); + // Add glue to push bin size label to the right + controlPanel.add(Box.createHorizontalGlue()); + + // Bin size label - right-justified + this.binSizeLabel = new JLabel(formatBinSize(binSize)); + this.binSizeLabel.setAlignmentY(Component.CENTER_ALIGNMENT); + controlPanel.add(this.binSizeLabel); + return controlPanel; } @@ -686,28 +730,74 @@ private void updateSliderFromValue(double value) { } /** - * Update the slider range based on min and max values from the filtered data. + * Throttled application of the slider value to the color scale. + * Ensures updates occur at most once every 100 milliseconds. */ - private void updateSliderRange(double minValue, double maxValue) { - this.sliderMinValue = minValue; - this.sliderMaxValue = maxValue; + private void applySliderValueThrottled(double value) { + final long THROTTLE_MS = 100; + synchronized (sliderThrottleLock) { + long now = System.currentTimeMillis(); + long elapsed = now - lastSliderApplyTime; + if (elapsed >= THROTTLE_MS) { + // Apply immediately + lastSliderApplyTime = now; + SwingUtilities.invokeLater(() -> applySliderValue(value)); + } else { + // Schedule the latest pending value to run after the remaining delay + pendingSliderValue = value; + int delay = (int) Math.max(1, (THROTTLE_MS - elapsed)); + if (sliderThrottleTimer != null && sliderThrottleTimer.isRunning()) { + sliderThrottleTimer.stop(); + } + sliderThrottleTimer = new javax.swing.Timer(delay, e -> { + double v; + synchronized (sliderThrottleLock) { + v = pendingSliderValue; + pendingSliderValue = Double.NaN; + lastSliderApplyTime = System.currentTimeMillis(); + } + applySliderValue(v); + }); + sliderThrottleTimer.setRepeats(false); + sliderThrottleTimer.start(); + } + } + } - // Update slider position to maintain current value if possible + /** + * Apply the slider value to the current color scale and repaint. Must be called on EDT. + */ + private void applySliderValue(double value) { String colorScaleKey = getColorScaleCacheKey(); ContinuousColorScale colorScale = this.colorScaleCache.get(colorScaleKey); if (colorScale != null) { - updateSliderFromValue(colorScale.getMaximum()); + colorScale.setPosEnd(value); + this.repaint(); } } + /** * Format bin size for display (e.g., 1000 -> "1 kb", 1000000 -> "1 Mb") + * Avoids using fractional kb values (e.g., "200 bp" instead of "0.2 kb") */ private static String formatBinSize(int binSize) { if (binSize >= 1000000) { - return String.format("%.1f Mb", binSize / 1000000.0); + // Format Mb - remove decimal if it's a whole number + double mb = binSize / 1000000.0; + if (mb == Math.floor(mb)) { + return String.format("%d Mb", (int) mb); + } else { + return String.format("%.1f Mb", mb); + } } else if (binSize >= 1000) { - return String.format("%.1f kb", binSize / 1000.0); + // Format kb - only use kb if >= 1 kb, remove decimal if it's a whole number + double kb = binSize / 1000.0; + if (kb == Math.floor(kb)) { + return String.format("%d kb", (int) kb); + } else { + return String.format("%.1f kb", kb); + } } else { return binSize + " bp"; } @@ -731,23 +821,23 @@ public void receiveEvent(IGVEvent event) { * * @return the percentile value, or -1 if contactRecords is empty */ - private double computePercentileOnly(List contactRecords, double percentile) { + private double initializeColorScale(List contactRecords) { if (contactRecords == null || contactRecords.isEmpty()) { - return -1; // Return -1 if there are no records + return 10; // no records } - // Filter records where Math.abs(bin2 - bin1) < 5 + // Filter records close to the diagonal List counts = contactRecords.stream() - .filter(record -> Math.abs(record.bin2() - record.bin1()) < 5) + .filter(record -> Math.abs(record.bin2() - record.bin1()) > 2) .map(ContactRecord::counts) .sorted() .collect(Collectors.toList()); if (counts.isEmpty()) { - return -1; // Return -1 if no records pass the filter + return 10; //no records pass the filter. Highly unlikely } - int index = (int) Math.ceil(percentile * counts.size()) - 1; + int index = (int) Math.ceil(.98 * counts.size()) - 1; float midCount = counts.get(index); // Update slider range based on min and max of filtered counts @@ -756,7 +846,6 @@ private double computePercentileOnly(List contactRecords, double this.sliderMinValue = minCount; this.sliderMaxValue = maxCount; - return midCount; } diff --git a/src/main/java/org/igv/bedpe/InteractionTrack.java b/src/main/java/org/igv/bedpe/InteractionTrack.java index c61a44be96..971e47300e 100644 --- a/src/main/java/org/igv/bedpe/InteractionTrack.java +++ b/src/main/java/org/igv/bedpe/InteractionTrack.java @@ -79,7 +79,7 @@ enum ArcOption {ALL, ONE_END, BOTH_ENDS} private boolean isHIC; ContactMapView contactMapView; - float transparency = 1.0f; + float transparency = 0.1f; String normalization = "NONE"; private int maxFeatureCount = 5000; @@ -110,10 +110,8 @@ public InteractionTrack(ResourceLocator locator, InteractionSource src) { if (isHIC) { graphType = GraphType.NESTED_ARC; useScore = true; - transparency = 0.1f; this.setColor(Color.red); } else { - String typeString = PreferencesManager.getPreferences().get(Constants.ARC_TYPE); if (typeString != null) { try { diff --git a/src/main/java/org/igv/bedpe/NestedArcRenderer.java b/src/main/java/org/igv/bedpe/NestedArcRenderer.java index 1719ec6f02..f1a8564814 100644 --- a/src/main/java/org/igv/bedpe/NestedArcRenderer.java +++ b/src/main/java/org/igv/bedpe/NestedArcRenderer.java @@ -10,11 +10,14 @@ import static org.igv.bedpe.InteractionTrack.Direction.UP; -public class NestedArcRenderer implements BedPERenderer{ +public class NestedArcRenderer implements BedPERenderer { private Map alphaColors = new HashMap<>(); + // Default track color to use when track.getColor() returns null + private static final Color DEFAULT_TRACK_COLOR = Color.blue; + InteractionTrack track; double theta = Math.toRadians(45); double sinTheta = Math.sin(theta); @@ -37,7 +40,7 @@ public void render(List features, g = (Graphics2D) context.getGraphics().create(); double origin = context.getOrigin(); double locScale = context.getScale(); - Color trackColor = track.getColor(); + Color trackColor = track.getColor() == null ? DEFAULT_TRACK_COLOR : track.getColor(); int gap = track.gap; if (track.thickness > 1) { @@ -75,16 +78,17 @@ public void render(List features, if (bedPE.isSameChr()) { BedPE feature = bedPE; - Color fcolor = feature.getColor() == null ? trackColor : feature.getColor(); double pixelStart = (feature.getMidStart() - origin) / locScale; double pixelEnd = (feature.getMidEnd() - origin) / locScale; // Optionally filter arcs with one or both ends out of view - if(arcOption == InteractionTrack.ArcOption.ONE_END) { - if(pixelStart < trackRectangle.x && pixelEnd > trackRectangle.x + trackRectangle.width) continue; - } else if(arcOption == InteractionTrack.ArcOption.BOTH_ENDS) { - if(pixelStart < trackRectangle.x || pixelEnd > trackRectangle.x + trackRectangle.width) continue; + if (arcOption == InteractionTrack.ArcOption.ONE_END) { + if (pixelStart < trackRectangle.x && pixelEnd > trackRectangle.x + trackRectangle.width) + continue; + } else if (arcOption == InteractionTrack.ArcOption.BOTH_ENDS) { + if (pixelStart < trackRectangle.x || pixelEnd > trackRectangle.x + trackRectangle.width) + continue; } int w = (int) (pixelEnd - pixelStart); @@ -94,16 +98,14 @@ public void render(List features, } - if (fcolor != null && w > trackRectangle.width && !track.useScore) { - fcolor = getAlphaColor(fcolor, 0.1f); - } else if (track.useScore) { - float alpha = track.transparency * Math.min(1.0f, Math.max(0.1f, feature.getScore() / 1000f)); - fcolor = getAlphaColor(fcolor, alpha); + Color fcolor = feature.getColor() == null ? trackColor : feature.getColor(); + float alpha = track.transparency; + if (track.useScore) { + alpha = track.transparency * Math.min(1.0f, Math.max(0.1f, feature.getScore() / 1000f)); } + fcolor = getAlphaColor(fcolor, alpha); + g.setColor(fcolor); - if (fcolor != null) { - g.setColor(fcolor); - } final int trackBaseLine = trackRectangle.y + trackRectangle.height; double a = w / 2; @@ -134,7 +136,7 @@ public void render(List features, } } - if(track.markerBounds != null) { + if (track.markerBounds != null) { int l1 = (int) ((track.markerBounds[0] - origin) / locScale); int l2 = (int) ((track.markerBounds[1] - origin) / locScale); g.setColor(Color.black); @@ -142,7 +144,7 @@ public void render(List features, g.drawLine(l2, trackRectangle.y, l2, trackRectangle.y + trackRectangle.height - 1); } } finally { - if(g != null) g.dispose(); + if (g != null) g.dispose(); } } @@ -215,7 +217,7 @@ public boolean contains(double x, double y) { double dx = x - xc; double dy = y - yc; - double dist = Math.sqrt(dx*dx + dy*dy); + double dist = Math.sqrt(dx * dx + dy * dy); return Math.abs(r - dist) <= 3; } } diff --git a/src/main/java/org/igv/bedpe/WrappedInteractionSource.java b/src/main/java/org/igv/bedpe/WrappedInteractionSource.java index ed1bfe2666..d0594b27cd 100644 --- a/src/main/java/org/igv/bedpe/WrappedInteractionSource.java +++ b/src/main/java/org/igv/bedpe/WrappedInteractionSource.java @@ -3,11 +3,12 @@ import org.igv.track.FeatureSource; import java.io.IOException; +import java.util.ArrayList; import java.util.Iterator; import java.util.List; -import java.util.ArrayList; +import java.util.concurrent.ThreadLocalRandom; -public class WrappedInteractionSource implements InteractionSource { +public class WrappedInteractionSource implements InteractionSource { FeatureSource featureSource; @@ -18,11 +19,38 @@ public WrappedInteractionSource(FeatureSource featureSource) { @Override public List getFeatures(String chr, int start, int end, double bpPerPixel, String normalization, int maxFeatureCount) throws IOException { - Iterator featureIterator = featureSource.getFeatures(chr, start, end); - List list = new ArrayList<>(); - while (featureIterator != null && featureIterator.hasNext()) { - list.add(featureIterator.next()); + Iterator featureIterator = featureSource.getFeatures(chr, start, end); + List reservoir = new ArrayList<>(); + + if (featureIterator == null) { + return reservoir; + } + + // If maxFeatureCount <= 0 treat as "no limit" and return all features + if (maxFeatureCount <= 0) { + while (featureIterator.hasNext()) { + reservoir.add(featureIterator.next()); + } + return reservoir; } - return list; + + int i = 0; // number of items seen so far + + while (featureIterator.hasNext()) { + BedPE item = featureIterator.next(); + if (i < maxFeatureCount) { + // fill the reservoir + reservoir.add(item); + } else { + // replace elements with decreasing probability + int r = ThreadLocalRandom.current().nextInt(i + 1); // 0..i + if (r < maxFeatureCount) { + reservoir.set(r, item); + } + } + i++; + } + + return reservoir; } } diff --git a/test/data/wig/test.bedgraph b/test/data/wig/test.bedgraph index ccb7e596ba..59c81a1880 100644 --- a/test/data/wig/test.bedgraph +++ b/test/data/wig/test.bedgraph @@ -1,10001 +1,10001 @@ track name=test -chr1 0 1000 0.9774505 -chr1 0 1000 6.236651E-4 -chr1 0 1000 0.5189936 -chr1 0 1000 0.05836807 -chr1 0 1000 0.44591993 -chr1 0 1000 0.6194714 -chr1 0 1000 0.39083064 -chr1 0 1000 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0.57629067 +chr1 0 1000 0.64634454 +chr1 0 1000 0.18819758 +chr1 0 1000 0.65232843 +chr1 0 1000 0.6433756 +chr1 0 1000 0.0111727305 +chr1 0 1000 0.005670894 +chr1 0 1000 0.7542305 +chr1 0 1000 0.11394839 +chr1 0 1000 0.17250237 +chr1 0 1000 0.6136736 +chr1 0 1000 0.6210474 +chr1 0 1000 0.7721754 +chr1 0 1000 0.5516654 +chr1 0 1000 0.10636305 +chr1 0 1000 0.95535165 From 30eb974fbcf02650b9577c4dfc119865385734b9 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Jan 2026 20:35:41 -0800 Subject: [PATCH 2/6] Refactor InteractionTrack to eliminate "isHIC" checks Other optimzations --- .../java/org/igv/bedpe/ContactMapView.java | 54 +- .../org/igv/bedpe/HicInteractionTrack.java | 135 + src/main/java/org/igv/bedpe/HicSource.java | 56 +- .../java/org/igv/bedpe/InteractionTrack.java | 202 +- src/main/java/org/igv/hic/ContactRecord.java | 7 +- src/main/java/org/igv/hic/HicFile.java | 7 +- .../org/igv/session/IGVSessionReader.java | 3 + src/main/java/org/igv/track/TrackLoader.java | 10 +- test/data/wig/test.bedgraph | 19998 ++++++++-------- 9 files changed, 10265 insertions(+), 10207 deletions(-) create mode 100644 src/main/java/org/igv/bedpe/HicInteractionTrack.java diff --git a/src/main/java/org/igv/bedpe/ContactMapView.java b/src/main/java/org/igv/bedpe/ContactMapView.java index 41d1fb2449..cf519d7770 100644 --- a/src/main/java/org/igv/bedpe/ContactMapView.java +++ b/src/main/java/org/igv/bedpe/ContactMapView.java @@ -31,8 +31,7 @@ public class ContactMapView extends JPanel implements IGVEventObserver { private HicFile hicFile; private String normalization; private Color color; - private Map colorScaleCache = new HashMap<>(); // Key: "normalization_binSize" - private Map colorScaleDataKeys = new HashMap<>(); // Tracks which data cache key was used for each color scale + private Map colorScaleCache = new HashMap<>(); // Key: "normalization_binSize", computed once per combination private ReferenceFrame frame; // Current genomic region frame (dynamic) private int binSize; // Bin size for the current genomic region (dynamic) private int startBin; // Start bin for the current genomic region (dynamic) @@ -117,7 +116,7 @@ public void mouseMoved(MouseEvent e) { .findFirst() .orElse(null); if (found != null) { - setToolTipText(String.format("Counts: %.2f", found.counts())); + setToolTipText(String.format("Counts: %d Normalized Counts: %.2f", (int) found.counts(), found.normCounts())); } else { setToolTipText(null); } @@ -463,43 +462,18 @@ private Image renderMapFromCache() { String colorScaleKey = getColorScaleCacheKey(); ContinuousColorScale colorScale = colorScaleCache.get(colorScaleKey); - // Check if the cached records match the current view parameters - // If they don't, we have stale data and shouldn't compute a new color scale yet - String currentCacheKey = generateCacheKey(); - boolean dataMatchesCurrentView = currentCacheKey.equals(dataCacheKey); - - // Check if this color scale was computed from the current data - String colorScaleDataKey = colorScaleDataKeys.get(colorScaleKey); - boolean colorScaleNeedsUpdate = colorScale == null || !currentCacheKey.equals(colorScaleDataKey); - - // We need to compute/recompute the color scale if: - // 1. No color scale exists for this key, OR - // 2. The color scale exists but was computed from different data - if (dataMatchesCurrentView && colorScaleNeedsUpdate) { - // We have fresh data and need to compute/update color scale from it - // First, compute the percentile and update slider range + // Create a color scale for this binSize + normalization combination if one doesn't exist. + // Once created, it persists and is only modified by user edits through the control panel. + if (colorScale == null) { + // Compute initial color scale from the current data double upper = initializeColorScale(cachedRecords); + Color lowerColor = Globals.isDarkMode() ? Color.BLACK : Color.WHITE; + colorScale = new ContinuousColorScale(0, upper, lowerColor, color); + colorScaleCache.put(colorScaleKey, colorScale); - if (colorScale == null) { - // Create new color scale - Color lowerColor = Globals.isDarkMode() ? Color.BLACK : Color.WHITE; - colorScale = new ContinuousColorScale(0, upper, lowerColor, color); - colorScaleCache.put(colorScaleKey, colorScale); - } else { - // Update existing color scale with new max value - colorScale.setPosEnd(upper); - } - - // Track that this color scale was computed from the current data - colorScaleDataKeys.put(colorScaleKey, currentCacheKey); - - // Now update text field and slider position based on the new color scale + // Update text field and slider position for the new color scale maxField.setText(String.format("%.2f", colorScale.getMaximum())); updateSliderFromValue(colorScale.getMaximum()); - } else if (colorScale == null) { - // We don't have fresh data and no color scale exists - // Just return null and wait for fresh data - return null; } @@ -513,7 +487,7 @@ private Image renderMapFromCache() { int y = (int) (binY * scaleFactor); if (x >= 0 && y >= 0) { - Color color = colorScale.getColor(record.counts()); + Color color = colorScale.getColor(record.normCounts()); // Calculate the size of each scaled bin int pixelSize = Math.max(1, (int) Math.ceil(scaleFactor)); @@ -826,10 +800,8 @@ private double initializeColorScale(List contactRecords) { return 10; // no records } - // Filter records close to the diagonal List counts = contactRecords.stream() - .filter(record -> Math.abs(record.bin2() - record.bin1()) > 2) - .map(ContactRecord::counts) + .map(ContactRecord::normCounts) .sorted() .collect(Collectors.toList()); @@ -842,7 +814,7 @@ private double initializeColorScale(List contactRecords) { // Update slider range based on min and max of filtered counts float minCount = counts.get(0); - float maxCount = 2 * midCount; + float maxCount = 4 * midCount; this.sliderMinValue = minCount; this.sliderMaxValue = maxCount; diff --git a/src/main/java/org/igv/bedpe/HicInteractionTrack.java b/src/main/java/org/igv/bedpe/HicInteractionTrack.java new file mode 100644 index 0000000000..17186a427b --- /dev/null +++ b/src/main/java/org/igv/bedpe/HicInteractionTrack.java @@ -0,0 +1,135 @@ +package org.igv.bedpe; + +import org.igv.hic.HicFile; +import org.igv.renderer.ContinuousColorScale; +import org.igv.track.RenderContext; +import org.igv.track.TrackClickEvent; +import org.igv.ui.panel.FrameManager; +import org.igv.ui.panel.IGVPopupMenu; +import org.igv.ui.panel.ReferenceFrame; +import org.igv.util.ResourceLocator; +import org.w3c.dom.Document; +import org.w3c.dom.Element; + +import javax.swing.*; +import java.awt.*; +import java.util.List; + +/** + * Subclass of InteractionTrack for HiC format files. + * Handles HiC-specific behavior like normalization, contact map views, and zoom-based filtering. + */ +public class HicInteractionTrack extends InteractionTrack { + + public HicInteractionTrack() { + super(); + } + + public HicInteractionTrack(ResourceLocator locator, HicSource source) { + super(locator, source); + // HiC-specific defaults + maxFeatureCount = 5000; + graphType = GraphType.NESTED_ARC; + useScore = true; + setColor(Color.red); + } + + @Override + protected List filterFeaturesForZoom(List features, LoadedInterval interval, ReferenceFrame referenceFrame) { + // In HiC mode we limit interactions to those in view plus a margin of one screen width to either side. + // If zooming in this means we have to filter the features from the previous zoom level that are outside + // of this range. Not doing so leads to inconsistent rendering when loading for the current zoom + // completes and repaints. + if (interval.zoom() < referenceFrame.getZoom()) { + int start = (int) referenceFrame.getOrigin(); + int end = (int) referenceFrame.getEnd(); + int w = (end - start); + int finalStart = start - w; + int finalEnd = end + w; + return features.stream() + .takeWhile(f -> f.getStart() <= finalEnd) + .filter(f -> f.getEnd() >= finalStart) + .toList(); + } + return features; + } + + @Override + protected void addFormatSpecificMenuItems(IGVPopupMenu menu, TrackClickEvent te) { + // Add normalization options for HiC tracks + List normalizationTypes = featureSource.getNormalizationTypes(); + if (normalizationTypes != null && normalizationTypes.size() > 1) { + menu.addSeparator(); + menu.add(new JLabel("Normalization")); + ButtonGroup normGroup = new ButtonGroup(); + for (String type : normalizationTypes) { + String label = normalizationLabels.getOrDefault(type, type); + JRadioButtonMenuItem normItem = new JRadioButtonMenuItem(label); + normItem.setSelected(type.equals(normalization)); + normItem.addActionListener(e -> { + this.normalization = type; + if (contactMapView != null) { + contactMapView.setNormalization(type); + } + this.repaint(); + }); + normGroup.add(normItem); + menu.add(normItem); + } + } + + menu.addSeparator(); + JMenuItem mapItem = new JMenuItem("Open Contact Map View"); + mapItem.setEnabled(contactMapView == null && !FrameManager.isGeneListMode()); + mapItem.addActionListener(e -> { + ReferenceFrame frame = te.getFrame() != null ? te.getFrame() : FrameManager.getDefaultFrame(); + if (contactMapView == null) { + ContinuousColorScale colorScale = this.getColorScale(); + HicFile hicFile = ((HicSource) featureSource).getHicFile(); + ContactMapView.showPopup(this, hicFile, normalization, frame, colorScale.getMaxColor()); + } + }); + menu.add(mapItem); + } + + @Override + protected boolean supportsGraphTypeSelection() { + return false; + } + + @Override + protected boolean supportsCircularView() { + return false; + } + + @Override + protected boolean supportsAutoscaleMenu() { + return false; + } + + @Override + protected boolean supportsFeatureWindowMenu() { + return false; + } + + @Override + public void marshalXML(Document document, Element element) { + super.marshalXML(document, element); + + String nviString = ((HicSource) featureSource).getNVIString(); + if (nviString != null) { + element.setAttribute("nvi", nviString); + } + } + + @Override + public void unmarshalXML(Element element, Integer version) { + super.unmarshalXML(element, version); + + if (element.hasAttribute("nvi")) { + String nviString = element.getAttribute("nvi"); + ((HicSource) featureSource).setNVIString(nviString); + } + } +} + diff --git a/src/main/java/org/igv/bedpe/HicSource.java b/src/main/java/org/igv/bedpe/HicSource.java index 1fff8bd8db..37ee11f9ea 100644 --- a/src/main/java/org/igv/bedpe/HicSource.java +++ b/src/main/java/org/igv/bedpe/HicSource.java @@ -7,7 +7,10 @@ import org.igv.hic.Region; import java.io.IOException; -import java.util.*; +import java.util.ArrayList; +import java.util.Collections; +import java.util.Comparator; +import java.util.List; import java.util.concurrent.ThreadLocalRandom; /** @@ -36,7 +39,7 @@ public List getFeatures(String chr, int start, int end, double bpPerPixel final int binSize = hicFile.getBinSize(chr, bpPerPixel); - List records = getRecords(chr, start, end, binSize); + List records = getRecords(chr, start, end, binSize, normalization); if (records.isEmpty()) { return Collections.emptyList(); @@ -52,7 +55,7 @@ public List getFeatures(String chr, int start, int end, double bpPerPixel int b1 = rec.bin1(); int b2 = rec.bin2(); if (Math.abs(b1 - b2) > this.binThreshold) { - values.add(rec.counts()); + values.add(rec.normCounts()); } if (b1 < binMin) binMin = b1; if (b2 < binMin) binMin = b2; @@ -60,7 +63,7 @@ public List getFeatures(String chr, int start, int end, double bpPerPixel if (b2 > binMax) binMax = b2; } - if(values.isEmpty()) { + if (values.isEmpty()) { return Collections.emptyList(); } @@ -78,7 +81,7 @@ public List getFeatures(String chr, int start, int end, double bpPerPixel int bin2 = rec.bin2(); if (Math.abs(bin1 - bin2) <= this.binThreshold) continue; - float counts = rec.counts(); + float counts = rec.normCounts(); if (counts > threshold) { significantRecords.add(rec); } else if (counts == threshold) { @@ -107,48 +110,17 @@ public List getFeatures(String chr, int start, int end, double bpPerPixel } } - double[] normVector = null; - boolean useNormalization = normalization != null && !"NONE".equals(normalization); - if (useNormalization) { - NormalizationVector nv = hicFile.getNormalizationVector(normalization, chr, "BP", binSize); - if (nv == null) { - useNormalization = false; - } else { - normVector = nv.getValues(binMin, binMax); - if (normVector == null) { - useNormalization = false; - } - } - } - // Convert contact records to features List features = new ArrayList<>(); String c = getChromosomeNameFromGenome(chr); for (ContactRecord rec : significantRecords) { - int bin1 = rec.bin1(); - int bin2 = rec.bin2(); - float value = rec.counts(); - if (useNormalization) { - double nvnv = 1; - try { - nvnv = normVector[bin1 - binMin] * normVector[bin2 - binMin]; - } catch (Exception e) { - throw new RuntimeException(e); - } - if (!Double.isNaN(nvnv)) { - value /= nvnv; - } else { - continue; - } - } - - int start1 = bin1 * binSize; + int start1 = rec.bin1() * binSize; int end1 = start1 + binSize; - int start2 = bin2 * binSize; + int start2 = rec.bin2() * binSize; int end2 = start2 + binSize; - HicFeature f = new HicFeature(c, start1, end1, c, start2, end2, rec.counts(), value); + HicFeature f = new HicFeature(c, start1, end1, c, start2, end2, rec.counts(), rec.normCounts()); int score = (max > min) ? (int) Math.round(200 + Math.min(Math.max((f.getValue() - min) / (max - min), 0), 1) * 600) : 800; f.setScore(score); features.add(f); @@ -181,7 +153,7 @@ public boolean hasNormalizationVector(String type, String chr, double bpPerPixel * @return * @throws IOException */ - private List getRecords(String chr, int start, int end, int binSize) throws IOException { + private List getRecords(String chr, int start, int end, int binSize, String normalization) throws IOException { final Region region1 = new Region(chr, start, end); List records = hicFile.getContactRecords( @@ -189,11 +161,11 @@ private List getRecords(String chr, int start, int end, int binSi region1, "BP", binSize, - "NONE", + normalization, false ); - if(start > 0) { + if (start > 0) { Region adjacent = new Region(chr, Math.max(0, start - (end - start)), start); List adjacentRecords = hicFile.getContactRecords(region1, adjacent, "BP", binSize, "NONE", false); records.addAll(adjacentRecords); diff --git a/src/main/java/org/igv/bedpe/InteractionTrack.java b/src/main/java/org/igv/bedpe/InteractionTrack.java index 971e47300e..49c47b5758 100644 --- a/src/main/java/org/igv/bedpe/InteractionTrack.java +++ b/src/main/java/org/igv/bedpe/InteractionTrack.java @@ -62,26 +62,25 @@ enum ArcOption {ALL, ONE_END, BOTH_ENDS} normalizationLabels.put("GW_KR", "Genome-wide Balanced"); } - private InteractionSource featureSource; + protected InteractionSource featureSource; private JCheckBoxMenuItem autoscaleCB; private JMenuItem maxScoreItem; InteractionTrack.Direction direction = UP; //DOWN; - GraphType graphType; // GraphType.block; // + protected GraphType graphType; // GraphType.block; // private ArcOption arcOption = ArcOption.ALL; int thickness = 1; boolean autoscale = true; double maxScore = -1; int gap = 5; boolean showBlocks = false; - boolean useScore = false; + protected boolean useScore = false; private Map renderers; - private boolean isHIC; ContactMapView contactMapView; float transparency = 0.1f; - String normalization = "NONE"; - private int maxFeatureCount = 5000; + protected String normalization = "NONE"; + protected int maxFeatureCount = 20000; int[] markerBounds = null; @@ -106,23 +105,16 @@ public InteractionTrack(ResourceLocator locator, InteractionSource src) { renderers.put(GraphType.PROPORTIONAL_ARC, new ProportionalArcRenderer(this)); renderers.put(GraphType.BLOCK, new PEBlockRenderer(this)); - this.isHIC = "hic".equals(locator.getFormat()); - if (isHIC) { - graphType = GraphType.NESTED_ARC; - useScore = true; - this.setColor(Color.red); - } else { - String typeString = PreferencesManager.getPreferences().get(Constants.ARC_TYPE); - if (typeString != null) { - try { - graphType = GraphType.valueOf(typeString); - } catch (IllegalArgumentException e) { - log.error("Illegal graph type: " + typeString, e); - graphType = GraphType.NESTED_ARC; // default - } - } else { - graphType = GraphType.PROPORTIONAL_ARC; + String typeString = PreferencesManager.getPreferences().get(Constants.ARC_TYPE); + if (typeString != null) { + try { + graphType = GraphType.valueOf(typeString); + } catch (IllegalArgumentException e) { + log.error("Illegal graph type: " + typeString, e); + graphType = GraphType.NESTED_ARC; // default } + } else { + graphType = GraphType.PROPORTIONAL_ARC; } String directionString = PreferencesManager.getPreferences().get(Constants.ARC_DIRECTION); @@ -227,24 +219,7 @@ public void render(RenderContext context, Rectangle trackRectangle) { } List features = interval.features(); - List filteredFeatures; - if (isHIC && interval.zoom < referenceFrame.getZoom()) { - // In hic mode we limit interactions to those in view plus a margin of one screen width to either side. - // If zooming in this means we have to filter the features from the previous zoom level that are outside - // of this range. Not doing so leads to inconsistent rendering when loading for the current zoom - // completes and repaints. - int start = (int) referenceFrame.getOrigin(); - int end = (int) referenceFrame.getEnd(); - int w = (end - start); - int finalStart = start - w; - int finalEnd = end + w; - filteredFeatures = features.stream() - .takeWhile(f -> f.getStart() <= finalEnd) - .filter(f -> f.getEnd() >= finalStart) - .toList(); - } else { - filteredFeatures = features; - } + List filteredFeatures = filterFeaturesForZoom(features, interval, referenceFrame); if (filteredFeatures != null && !filteredFeatures.isEmpty()) { @@ -261,7 +236,7 @@ public void render(RenderContext context, Rectangle trackRectangle) { if (showBlocks) { renderers.get(GraphType.BLOCK).render(filteredFeatures, context, trackRectangle, this.arcOption); } - if(contactMapView != null && !FrameManager.isGeneListMode()) { + if (contactMapView != null && !FrameManager.isGeneListMode()) { } } finally { @@ -309,6 +284,14 @@ private double autoscale(List features) { return maxScore; } + /** + * Hook method for subclasses to filter features based on zoom level. + * Default implementation returns features unchanged. + */ + protected List filterFeaturesForZoom(List features, LoadedInterval interval, ReferenceFrame referenceFrame) { + return features; + } + @Override public IGVPopupMenu getPopupMenu(TrackClickEvent te) { @@ -325,7 +308,7 @@ public IGVPopupMenu getPopupMenu(TrackClickEvent te) { item.addActionListener(evt -> TrackMenuUtils.changeTrackColor(Collections.singleton(InteractionTrack.this))); menu.add(item); - if (!this.isHIC) { + if (supportsGraphTypeSelection()) { menu.addSeparator(); menu.add(new JLabel("Graph Type")); //enum GraphType {BLOCK, ARC, PROPORTIONAL_ARC} @@ -380,7 +363,7 @@ public IGVPopupMenu getPopupMenu(TrackClickEvent te) { }); menu.add(showBlocksCB); - if (!isHIC) { + if (supportsAutoscaleMenu()) { menu.addSeparator(); autoscaleCB = new JCheckBoxMenuItem("Autoscale"); autoscaleCB.setSelected(autoscale); @@ -467,73 +450,38 @@ public IGVPopupMenu getPopupMenu(TrackClickEvent te) { }); menu.add(transparencyItem); - if (isHIC) { - final JMenuItem maxFeatureCountItem = new JMenuItem("Set Max Feature Count..."); - maxFeatureCountItem.addActionListener(e -> { - final JSlider slider = new JSlider(1000, 20000, InteractionTrack.this.maxFeatureCount); - slider.setMajorTickSpacing(5000); - slider.setPaintTicks(true); - - final JLabel valueLabel = new JLabel(String.valueOf(InteractionTrack.this.maxFeatureCount)); - - slider.addChangeListener(changeEvent -> { - JSlider source = (JSlider) changeEvent.getSource(); - int value = source.getValue(); - InteractionTrack.this.maxFeatureCount = value; - valueLabel.setText(String.valueOf(value)); - InteractionTrack.this.loadedIntervalMap.clear(); - InteractionTrack.this.repaint(); - }); - JPanel panel = new JPanel(new BorderLayout()); - panel.add(slider, BorderLayout.CENTER); - panel.add(valueLabel, BorderLayout.SOUTH); + final JMenuItem maxFeatureCountItem = new JMenuItem("Set Maximum Feature Count..."); + maxFeatureCountItem.addActionListener(e -> { + final JSlider slider = new JSlider(1000, 20000, InteractionTrack.this.maxFeatureCount); + slider.setMajorTickSpacing(5000); + slider.setPaintTicks(true); + + final JLabel valueLabel = new JLabel(String.valueOf(InteractionTrack.this.maxFeatureCount)); - final Frame parent = IGV.hasInstance() ? IGV.getInstance().getMainFrame() : null; - JOptionPane.showMessageDialog(parent, panel, "Set Max Feature Count for " + InteractionTrack.this.getDisplayName(), JOptionPane.PLAIN_MESSAGE); + slider.addChangeListener(changeEvent -> { + JSlider source = (JSlider) changeEvent.getSource(); + int value = source.getValue(); + InteractionTrack.this.maxFeatureCount = value; + valueLabel.setText(String.valueOf(value)); + InteractionTrack.this.loadedIntervalMap.clear(); + InteractionTrack.this.repaint(); }); - menu.add(maxFeatureCountItem); - } - // Add normalization options for HiC tracks - if (isHIC) { - List normalizationTypes = featureSource.getNormalizationTypes(); - if (normalizationTypes != null && normalizationTypes.size() > 1) { - menu.addSeparator(); - menu.add(new JLabel("Normalization")); - ButtonGroup normGroup = new ButtonGroup(); - for (String type : normalizationTypes) { - String label = normalizationLabels.getOrDefault(type, type); - JRadioButtonMenuItem normItem = new JRadioButtonMenuItem(label); - normItem.setSelected(type.equals(normalization)); - normItem.addActionListener(e -> { - this.normalization = type; - if(contactMapView != null) { - contactMapView.setNormalization(type); - } - InteractionTrack.this.repaint(); + JPanel panel = new JPanel(new BorderLayout()); + panel.add(slider, BorderLayout.CENTER); + panel.add(valueLabel, BorderLayout.SOUTH); - }); - normGroup.add(normItem); - menu.add(normItem); - } - } + final Frame parent = IGV.hasInstance() ? IGV.getInstance().getMainFrame() : null; + JOptionPane.showMessageDialog(parent, panel, "Set Max Feature Count for " + InteractionTrack.this.getDisplayName(), JOptionPane.PLAIN_MESSAGE); + }); + menu.add(maxFeatureCountItem); - menu.addSeparator(); - JMenuItem mapItem = new JMenuItem("Open Contact Map View"); - mapItem.setEnabled(contactMapView == null && !FrameManager.isGeneListMode()); - mapItem.addActionListener(e -> { - ReferenceFrame frame = te.getFrame() != null ? te.getFrame() : FrameManager.getDefaultFrame(); - if (contactMapView == null) { - ContinuousColorScale colorScale = this.getColorScale(); - - HicFile hicFile = ((HicSource) featureSource).getHicFile(); - ContactMapView.showPopup(this, hicFile, normalization, frame, colorScale.getMaxColor()); - } - }); - menu.add(mapItem); - } else { + // Hook for subclass-specific menu items (e.g., HiC normalization options) + addFormatSpecificMenuItems(menu, te); + + if (supportsFeatureWindowMenu()) { menu.addSeparator(); menu.add(TrackMenuUtils.getChangeFeatureWindow(Collections.singletonList(this))); } @@ -541,7 +489,7 @@ public IGVPopupMenu getPopupMenu(TrackClickEvent te) { // Experimental JBrowse. if (PreferencesManager.getPreferences().getAsBoolean(Constants.CIRC_VIEW_ENABLED) && CircularViewUtilities.ping() && - !isHIC) { + supportsCircularView()) { menu.addSeparator(); JMenuItem circViewItem = new JMenuItem("Add Features to Circular View"); circViewItem.addActionListener(e -> { @@ -563,6 +511,42 @@ private void setGraphType(GraphType value) { this.graphType = value; } + /** + * Hook method for subclasses to add format-specific menu items. + * Default implementation does nothing. + */ + protected void addFormatSpecificMenuItems(IGVPopupMenu menu, TrackClickEvent te) { + // Default: no additional menu items + } + + /** + * Hook method for subclasses to indicate if graph type selection is supported. + */ + protected boolean supportsGraphTypeSelection() { + return true; + } + + /** + * Hook method for subclasses to indicate if circular view is supported. + */ + protected boolean supportsCircularView() { + return true; + } + + /** + * Hook method for subclasses to indicate if autoscale menu is supported. + */ + protected boolean supportsAutoscaleMenu() { + return true; + } + + /** + * Hook method for subclasses to indicate if feature window menu is supported. + */ + protected boolean supportsFeatureWindowMenu() { + return true; + } + @Override public String getValueStringAt(String chr, double position, int mouseX, int mouseY, ReferenceFrame frame) { @@ -616,12 +600,6 @@ public void marshalXML(Document document, Element element) { if (!autoscale) { element.setAttribute("maxScore", String.valueOf(maxScore)); } - if (this.isHIC) { - String nviString = ((HicSource) featureSource).getNVIString(); - if (nviString != null) { - element.setAttribute("nvi", nviString); - } - } } @@ -654,10 +632,6 @@ public void unmarshalXML(Element element, Integer version) { if (element.hasAttribute("transparency")) { this.transparency = Float.parseFloat(element.getAttribute("transparency")); } - if (element.hasAttribute("nvi")) { - String nviString = element.getAttribute("nvi"); - ((HicSource) featureSource).setNVIString(nviString); - } } diff --git a/src/main/java/org/igv/hic/ContactRecord.java b/src/main/java/org/igv/hic/ContactRecord.java index 700715e65b..f9efc16a75 100644 --- a/src/main/java/org/igv/hic/ContactRecord.java +++ b/src/main/java/org/igv/hic/ContactRecord.java @@ -1,6 +1,11 @@ package org.igv.hic; -public record ContactRecord(int bin1, int bin2, float counts) { +public record ContactRecord(int bin1, int bin2, float counts, float normCounts) { + + public ContactRecord(int bin1, int bin2, float counts) { + this(bin1, bin2, counts, counts); + } + public String getKey() { return bin1 + "_" + bin2; } diff --git a/src/main/java/org/igv/hic/HicFile.java b/src/main/java/org/igv/hic/HicFile.java index 6d5555a1ab..a5e50211b8 100644 --- a/src/main/java/org/igv/hic/HicFile.java +++ b/src/main/java/org/igv/hic/HicFile.java @@ -311,13 +311,12 @@ public List getContactRecords(Region region1, if (normVector == null) { contactRecords.add(rec); } else { - float value = rec.counts(); double nvnv = normVector[rec.bin1() - binMin] * normVector[rec.bin2() - binMin]; if (!Double.isNaN(nvnv)) { - value /= nvnv; - ContactRecord normRec = new ContactRecord(rec.bin1(), rec.bin2(), value); + float normCounts = (float) (rec.counts() / nvnv); + ContactRecord normRec = new ContactRecord(rec.bin1(), rec.bin2(), rec.counts(), normCounts); contactRecords.add(normRec); - } + } } } } diff --git a/src/main/java/org/igv/session/IGVSessionReader.java b/src/main/java/org/igv/session/IGVSessionReader.java index 5dc12f5dcc..57c550dd77 100644 --- a/src/main/java/org/igv/session/IGVSessionReader.java +++ b/src/main/java/org/igv/session/IGVSessionReader.java @@ -1,6 +1,7 @@ package org.igv.session; import org.igv.Globals; +import org.igv.bedpe.HicInteractionTrack; import org.igv.bedpe.InteractionTrack; import org.igv.data.CombinedDataSource; import org.igv.feature.Locus; @@ -1088,6 +1089,8 @@ private Track createTrack(String className, Element element) { return new MotifTrack(); } else if (className.contains("GisticTrack")) { return new GisticTrack(); + } else if (className.contains("HicInteractionTrack")) { + return new HicInteractionTrack(); } else if (className.contains("InteractionTrack")) { return new InteractionTrack(); } else if (className.contains("MergedTracks")) { diff --git a/src/main/java/org/igv/track/TrackLoader.java b/src/main/java/org/igv/track/TrackLoader.java index 5f50a63514..3b8b0760da 100644 --- a/src/main/java/org/igv/track/TrackLoader.java +++ b/src/main/java/org/igv/track/TrackLoader.java @@ -412,19 +412,17 @@ private void loadBedPEFile(ResourceLocator locator, List newTracks, Genom String format = locator.getFormat(); - InteractionSource source; if("hic".equals(format)) { - source = new HicSource(locator.getPath(), genome); + HicSource source = new HicSource(locator.getPath(), genome); + newTracks.add(new HicInteractionTrack(locator, source)); } else { List features = "interact".equals(format) ? InteractParser.parse(locator, genome) : BedPEParser.parse(locator, genome); BedPESource featureSource = new BedPESource(features, genome); - source = new WrappedInteractionSource(featureSource); + InteractionSource source = new WrappedInteractionSource(featureSource); + newTracks.add(new InteractionTrack(locator, source)); } - - - newTracks.add(new InteractionTrack(locator, source)); } private void loadClusterFile(ResourceLocator locator, List newTracks, Genome genome) throws IOException { diff --git a/test/data/wig/test.bedgraph b/test/data/wig/test.bedgraph index 59c81a1880..e5930dc1d3 100644 --- a/test/data/wig/test.bedgraph +++ b/test/data/wig/test.bedgraph @@ -1,10001 +1,10001 @@ track name=test -chr1 0 1000 0.6913674 -chr1 0 1000 0.93226665 -chr1 0 1000 0.25581673 -chr1 0 1000 0.08136221 -chr1 0 1000 0.047030322 -chr1 0 1000 0.8651136 -chr1 0 1000 0.83838075 -chr1 0 1000 0.8120976 -chr1 0 1000 0.5544607 -chr1 0 1000 0.036928464 -chr1 0 1000 0.5055702 -chr1 0 1000 0.78823924 -chr1 0 1000 0.81790304 -chr1 0 1000 0.25243053 -chr1 0 1000 0.95392805 -chr1 0 1000 0.9455672 -chr1 0 1000 0.6145443 -chr1 0 1000 0.25274953 -chr1 0 1000 0.13043612 -chr1 0 1000 0.90251625 -chr1 0 1000 0.68173045 -chr1 0 1000 0.58573043 -chr1 0 1000 0.03276502 -chr1 0 1000 0.56446517 -chr1 0 1000 0.5858307 -chr1 0 1000 0.06527843 -chr1 0 1000 0.3005982 -chr1 0 1000 0.9397009 -chr1 0 1000 0.7041502 -chr1 0 1000 0.5076401 -chr1 0 1000 0.8428112 -chr1 0 1000 0.50973946 -chr1 0 1000 0.5671161 -chr1 0 1000 0.5936508 -chr1 0 1000 0.1642333 -chr1 0 1000 0.98594266 -chr1 0 1000 0.68002844 -chr1 0 1000 0.7733605 -chr1 0 1000 0.6309126 -chr1 0 1000 0.17987901 -chr1 0 1000 0.47199097 -chr1 0 1000 0.103083216 -chr1 0 1000 0.6442539 -chr1 0 1000 0.016633483 -chr1 0 1000 0.20420423 -chr1 0 1000 0.7847979 -chr1 0 1000 0.5820899 -chr1 0 1000 0.4282798 -chr1 0 1000 0.5106262 -chr1 0 1000 0.9639617 -chr1 0 1000 0.4086956 -chr1 0 1000 0.9789855 -chr1 0 1000 0.74107444 -chr1 0 1000 0.034179553 -chr1 0 1000 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0.7222783 +chr1 0 1000 0.40134183 +chr1 0 1000 0.42933914 +chr1 0 1000 0.36385694 +chr1 0 1000 0.08535156 +chr1 0 1000 0.24001536 +chr1 0 1000 0.24294017 +chr1 0 1000 0.38154113 +chr1 0 1000 0.37918657 +chr1 0 1000 0.99691874 +chr1 0 1000 0.7794913 +chr1 0 1000 0.5491733 +chr1 0 1000 0.5728787 +chr1 0 1000 0.71502936 +chr1 0 1000 0.5608491 +chr1 0 1000 0.6187245 +chr1 0 1000 0.21817204 +chr1 0 1000 0.25637674 +chr1 0 1000 0.2403697 +chr1 0 1000 0.3181168 +chr1 0 1000 0.35207504 +chr1 0 1000 0.2110083 +chr1 0 1000 0.9340972 +chr1 0 1000 0.4050428 +chr1 0 1000 0.86414206 +chr1 0 1000 0.13928054 +chr1 0 1000 0.86410517 +chr1 0 1000 0.6107059 +chr1 0 1000 0.50118023 +chr1 0 1000 0.55290157 +chr1 0 1000 0.7743506 +chr1 0 1000 0.32666042 +chr1 0 1000 0.2338992 +chr1 0 1000 0.47472128 +chr1 0 1000 0.7092788 +chr1 0 1000 0.02562723 +chr1 0 1000 0.66803026 +chr1 0 1000 0.5080862 +chr1 0 1000 0.78155434 +chr1 0 1000 0.7011053 +chr1 0 1000 0.54669744 +chr1 0 1000 0.0936454 +chr1 0 1000 0.4976728 +chr1 0 1000 0.060795218 +chr1 0 1000 0.16133232 +chr1 0 1000 0.034815248 +chr1 0 1000 0.058843065 +chr1 0 1000 0.8152955 +chr1 0 1000 0.6651817 +chr1 0 1000 0.43268025 +chr1 0 1000 0.80941176 +chr1 0 1000 0.16622862 +chr1 0 1000 0.86646426 +chr1 0 1000 0.8869282 +chr1 0 1000 0.6662339 +chr1 0 1000 0.5168983 +chr1 0 1000 0.06749202 +chr1 0 1000 0.19148746 +chr1 0 1000 0.9297775 +chr1 0 1000 0.50377506 +chr1 0 1000 0.9115785 +chr1 0 1000 0.7818552 From 8ed0b661a4fc5e8efb384d3159bd08694e2af409 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Jan 2026 21:13:44 -0800 Subject: [PATCH 3/6] Refactor InteractionTrack to eliminate "isHIC" checks Other optimzations --- .../java/org/igv/bedpe/ContactMapView.java | 47 ++++++++++++++----- .../org/igv/bedpe/HicInteractionTrack.java | 2 +- src/main/java/org/igv/hic/HicFile.java | 13 ++++- test/sessions/4dn_hic.xml | 6 +-- 4 files changed, 52 insertions(+), 16 deletions(-) diff --git a/src/main/java/org/igv/bedpe/ContactMapView.java b/src/main/java/org/igv/bedpe/ContactMapView.java index cf519d7770..bc1e81f534 100644 --- a/src/main/java/org/igv/bedpe/ContactMapView.java +++ b/src/main/java/org/igv/bedpe/ContactMapView.java @@ -31,6 +31,7 @@ public class ContactMapView extends JPanel implements IGVEventObserver { private HicFile hicFile; private String normalization; private Color color; + private Color backgroundColor; // Background color for the contact map, based on light/dark mode private Map colorScaleCache = new HashMap<>(); // Key: "normalization_binSize", computed once per combination private ReferenceFrame frame; // Current genomic region frame (dynamic) private int binSize; // Bin size for the current genomic region (dynamic) @@ -68,6 +69,7 @@ public ContactMapView(InteractionTrack track, HicFile hicFile, String normalizat this.normalization = normalization; this.frame = frame; this.color = color; + this.backgroundColor = Globals.isDarkMode() ? Color.BLACK : Color.WHITE; binSize = hicFile.getBinSize(frame.getChrName(), frame.getScale()); startBin = (int) (frame.getOrigin() / binSize); @@ -199,6 +201,9 @@ private void fetchDataAsync() { final int currentBinSize = binSize; final String fetchCacheKey = newCacheKey; + // Threshold on contact records. + int countThreshold = getCountThreshold(binSize); + currentFetchTask = dataFetchExecutor.submit(() -> { try { Region region = new Region(chrName, origin, end); @@ -208,7 +213,8 @@ private void fetchDataAsync() { "BP", currentBinSize, normalization, - true + true, + countThreshold ); // Update cache on EDT to avoid race conditions @@ -239,6 +245,20 @@ private void fetchDataAsync() { }); } + /** + * Determine count threshold based on bin size. Larger bin sizes get higher thresholds to reduce noise. + * Linear mapping: binSize 5000 → 0, binSize 250000 → 10, everything above 250000 → 10 + * + * @param binSize + * @return + */ + private static int getCountThreshold(int binSize) { + if (binSize <= 5000) return 0; + if (binSize >= 250000) return 10; + // Linear interpolation: threshold = (binSize - 5000) * 10 / (250000 - 5000) + return (int) Math.round((binSize - 5000) * 10.0 / 245000.0); + } + /** * Shutdown the executor service. Should be called when the view is disposed. */ @@ -456,6 +476,12 @@ private Image renderMapFromCache() { BufferedImage img = new BufferedImage(width, height, BufferedImage.TYPE_INT_ARGB); + // Fill the entire image with the background color (lowerColor) + Graphics2D g2d = img.createGraphics(); + g2d.setColor(backgroundColor); + g2d.fillRect(0, 0, width, height); + g2d.dispose(); + // Calculate scale factor for rendering double scaleFactor = (double) width / nBins; @@ -467,8 +493,7 @@ private Image renderMapFromCache() { if (colorScale == null) { // Compute initial color scale from the current data double upper = initializeColorScale(cachedRecords); - Color lowerColor = Globals.isDarkMode() ? Color.BLACK : Color.WHITE; - colorScale = new ContinuousColorScale(0, upper, lowerColor, color); + colorScale = new ContinuousColorScale(0, upper, backgroundColor, color); colorScaleCache.put(colorScaleKey, colorScale); // Update text field and slider position for the new color scale @@ -599,16 +624,16 @@ private JPanel createControlPanel() { this.maxSlider.setAlignmentY(Component.CENTER_ALIGNMENT); this.maxSlider.setToolTipText(String.format("Range: %.2f - %.2f", sliderMinValue, sliderMaxValue)); this.maxSlider.addChangeListener(e -> { - // Map slider value (0-100) to actual range (sliderMinValue to sliderMaxValue) - double value = sliderMinValue + (sliderMaxValue - sliderMinValue) * this.maxSlider.getValue() / 100.0; - this.maxField.setText(String.format("%.2f", value)); + // Map slider value (0-100) to actual range (sliderMinValue to sliderMaxValue) + double value = sliderMinValue + (sliderMaxValue - sliderMinValue) * this.maxSlider.getValue() / 100.0; + this.maxField.setText(String.format("%.2f", value)); - // Update tooltip to show current value - this.maxSlider.setToolTipText(String.format("Value: %.2f (Range: %.2f - %.2f)", - value, sliderMinValue, sliderMaxValue)); + // Update tooltip to show current value + this.maxSlider.setToolTipText(String.format("Value: %.2f (Range: %.2f - %.2f)", + value, sliderMinValue, sliderMaxValue)); - // Throttled update of the color scale to avoid excessive updates while dragging - applySliderValueThrottled(value); + // Throttled update of the color scale to avoid excessive updates while dragging + applySliderValueThrottled(value); }); controlPanel.add(this.maxSlider); diff --git a/src/main/java/org/igv/bedpe/HicInteractionTrack.java b/src/main/java/org/igv/bedpe/HicInteractionTrack.java index 17186a427b..f6302133d1 100644 --- a/src/main/java/org/igv/bedpe/HicInteractionTrack.java +++ b/src/main/java/org/igv/bedpe/HicInteractionTrack.java @@ -79,7 +79,7 @@ protected void addFormatSpecificMenuItems(IGVPopupMenu menu, TrackClickEvent te) } menu.addSeparator(); - JMenuItem mapItem = new JMenuItem("Open Contact Map View"); + JMenuItem mapItem = new JMenuItem("Contact Map View..."); mapItem.setEnabled(contactMapView == null && !FrameManager.isGeneListMode()); mapItem.addActionListener(e -> { ReferenceFrame frame = te.getFrame() != null ? te.getFrame() : FrameManager.getDefaultFrame(); diff --git a/src/main/java/org/igv/hic/HicFile.java b/src/main/java/org/igv/hic/HicFile.java index a5e50211b8..6f6286fa96 100644 --- a/src/main/java/org/igv/hic/HicFile.java +++ b/src/main/java/org/igv/hic/HicFile.java @@ -262,6 +262,17 @@ public List getContactRecords(Region region1, int binSize, String normalization, boolean allRecords) throws IOException { + return this.getContactRecords(region1, region2, units, binSize, normalization, allRecords, 1); + + } + + public List getContactRecords(Region region1, + Region region2, + String units, + int binSize, + String normalization, + boolean allRecords, + int countsTreshold) throws IOException { int idx1 = chromosomeIndexMap.getOrDefault(getFileChrName(region1.chr()), -1); int idx2 = chromosomeIndexMap.getOrDefault(getFileChrName(region2.chr()), -1); @@ -307,7 +318,7 @@ public List getContactRecords(Region region1, } for (ContactRecord rec : block.records) { - if (allRecords || (rec.bin1() >= x1 && rec.bin1() < x2 && rec.bin2() >= y1 && rec.bin2() < y2) && rec.counts() > 1) { + if ((allRecords || (rec.bin1() >= x1 && rec.bin1() < x2 && rec.bin2() >= y1 && rec.bin2() < y2)) && rec.counts() > countsTreshold) { if (normVector == null) { contactRecords.add(rec); } else { diff --git a/test/sessions/4dn_hic.xml b/test/sessions/4dn_hic.xml index d733b94150..6011edbe8d 100644 --- a/test/sessions/4dn_hic.xml +++ b/test/sessions/4dn_hic.xml @@ -3,13 +3,13 @@ - - + + - + From b9f22acb8c19eb6a657ef95a56b32049b2a3acca Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Jan 2026 21:17:30 -0800 Subject: [PATCH 4/6] ... --- src/main/java/org/igv/bedpe/ContactMapView.java | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/src/main/java/org/igv/bedpe/ContactMapView.java b/src/main/java/org/igv/bedpe/ContactMapView.java index bc1e81f534..fa35481200 100644 --- a/src/main/java/org/igv/bedpe/ContactMapView.java +++ b/src/main/java/org/igv/bedpe/ContactMapView.java @@ -512,7 +512,7 @@ private Image renderMapFromCache() { int y = (int) (binY * scaleFactor); if (x >= 0 && y >= 0) { - Color color = colorScale.getColor(record.normCounts()); + Color featureColor = colorScale.getColor(record.normCounts()); // Calculate the size of each scaled bin int pixelSize = Math.max(1, (int) Math.ceil(scaleFactor)); @@ -521,11 +521,11 @@ private Image renderMapFromCache() { for (int dx = 0; dx < pixelSize && (x + dx) < width; dx++) { for (int dy = 0; dy < pixelSize && (y + dy) < height; dy++) { if ((x + dx) < width && (y + dy) < height) { - img.setRGB(x + dx, y + dy, color.getRGB()); + img.setRGB(x + dx, y + dy, featureColor.getRGB()); } // Mirror for symmetry if ((y + dx) < width && (x + dy) < height) { - img.setRGB(y + dx, x + dy, color.getRGB()); + img.setRGB(y + dx, x + dy, featureColor.getRGB()); } } } From 9d81ce206bd3027d3ace226cec59d22fad54605a Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Jan 2026 22:17:03 -0800 Subject: [PATCH 5/6] Fixes for whole genome view Disable map tooltext -- very costly with current design --- .../java/org/igv/bedpe/ContactMapView.java | 76 ++++++++++++------- 1 file changed, 48 insertions(+), 28 deletions(-) diff --git a/src/main/java/org/igv/bedpe/ContactMapView.java b/src/main/java/org/igv/bedpe/ContactMapView.java index fa35481200..f1e9822368 100644 --- a/src/main/java/org/igv/bedpe/ContactMapView.java +++ b/src/main/java/org/igv/bedpe/ContactMapView.java @@ -109,22 +109,23 @@ public void mouseMoved(MouseEvent e) { track.setMarkerBounds(new int[]{coordX, coordY}); // Lookup a contact record for these bins (or the transpose) and update tooltip - if (cachedRecords != null) { - final int searchBin1 = binX; - final int searchBin2 = binY; - ContactRecord found = cachedRecords.stream() - .filter(r -> (r.bin1() == searchBin1 && r.bin2() == searchBin2) || - (r.bin1() == searchBin2 && r.bin2() == searchBin1)) - .findFirst() - .orElse(null); - if (found != null) { - setToolTipText(String.format("Counts: %d Normalized Counts: %.2f", (int) found.counts(), found.normCounts())); - } else { - setToolTipText(null); - } - } else { - setToolTipText(null); - } + // Disabled for performance reasons, revist with a better algorithm +// if (cachedRecords != null) { +// final int searchBin1 = binX; +// final int searchBin2 = binY; +// ContactRecord found = cachedRecords.stream() +// .filter(r -> (r.bin1() == searchBin1 && r.bin2() == searchBin2) || +// (r.bin1() == searchBin2 && r.bin2() == searchBin1)) +// .findFirst() +// .orElse(null); +// if (found != null) { +// setToolTipText(String.format("Counts: %d Normalized Counts: %.2f", (int) found.counts(), found.normCounts())); +// } else { +// setToolTipText(null); +// } +// } else { +// setToolTipText(null); +// } //String tooltip = String.format("Bin1: %d (Coord: %d), Bin2: %d (Coord: %d)", binX, coordX, binY, coordY); //setToolTipText(tooltip); @@ -202,7 +203,14 @@ private void fetchDataAsync() { final String fetchCacheKey = newCacheKey; // Threshold on contact records. - int countThreshold = getCountThreshold(binSize); + int countThreshold; + if (chrName.equalsIgnoreCase("all")) { + // For "all" chromosomes, scale bin size by 1000x to reduce data volume + countThreshold = 100; + } else { + countThreshold = getCountThreshold(binSize); + } + currentFetchTask = dataFetchExecutor.submit(() -> { try { @@ -780,25 +788,31 @@ private void applySliderValue(double value) { * Format bin size for display (e.g., 1000 -> "1 kb", 1000000 -> "1 Mb") * Avoids using fractional kb values (e.g., "200 bp" instead of "0.2 kb") */ - private static String formatBinSize(int binSize) { - if (binSize >= 1000000) { + private String formatBinSize(int binSize) { + + int actualBinSize = binSize; + if (frame.getChrName().equalsIgnoreCase("all")) { + actualBinSize *= 1000; + } + + if (actualBinSize >= 1000000) { // Format Mb - remove decimal if it's a whole number - double mb = binSize / 1000000.0; + double mb = actualBinSize / 1000000.0; if (mb == Math.floor(mb)) { return String.format("%d Mb", (int) mb); } else { return String.format("%.1f Mb", mb); } - } else if (binSize >= 1000) { + } else if (actualBinSize >= 1000) { // Format kb - only use kb if >= 1 kb, remove decimal if it's a whole number - double kb = binSize / 1000.0; + double kb = actualBinSize / 1000.0; if (kb == Math.floor(kb)) { return String.format("%d kb", (int) kb); } else { return String.format("%.1f kb", kb); } } else { - return binSize + " bp"; + return actualBinSize + " bp"; } } @@ -830,16 +844,22 @@ private double initializeColorScale(List contactRecords) { .sorted() .collect(Collectors.toList()); - if (counts.isEmpty()) { - return 10; //no records pass the filter. Highly unlikely + double percentile; + if (frame.getChrName().equalsIgnoreCase("all")) { + percentile = 0.98; + } else if (binSize < 1000) { + percentile = 0.0; + } else if (binSize <= 10000) { + percentile = 0.9; + } else { + percentile = 0.8; } - int index = (int) Math.ceil(.98 * counts.size()) - 1; + int index = (int) Math.ceil(percentile * (counts.size() - 1)); float midCount = counts.get(index); - - // Update slider range based on min and max of filtered counts float minCount = counts.get(0); float maxCount = 4 * midCount; + this.sliderMinValue = minCount; this.sliderMaxValue = maxCount; From e6d0baf991847920713f8ae1019ca18ff3ede198 Mon Sep 17 00:00:00 2001 From: jrobinso <933148+jrobinso@users.noreply.github.com> Date: Thu, 8 Jan 2026 22:29:22 -0800 Subject: [PATCH 6/6] fix typo, unit test --- src/main/java/org/igv/hic/HicFile.java | 4 ++-- src/test/java/org/igv/hic/HicFileTest.java | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/src/main/java/org/igv/hic/HicFile.java b/src/main/java/org/igv/hic/HicFile.java index 6f6286fa96..b26a343cfe 100644 --- a/src/main/java/org/igv/hic/HicFile.java +++ b/src/main/java/org/igv/hic/HicFile.java @@ -272,7 +272,7 @@ public List getContactRecords(Region region1, int binSize, String normalization, boolean allRecords, - int countsTreshold) throws IOException { + int countsThreshold) throws IOException { int idx1 = chromosomeIndexMap.getOrDefault(getFileChrName(region1.chr()), -1); int idx2 = chromosomeIndexMap.getOrDefault(getFileChrName(region2.chr()), -1); @@ -318,7 +318,7 @@ public List getContactRecords(Region region1, } for (ContactRecord rec : block.records) { - if ((allRecords || (rec.bin1() >= x1 && rec.bin1() < x2 && rec.bin2() >= y1 && rec.bin2() < y2)) && rec.counts() > countsTreshold) { + if ((allRecords || (rec.bin1() >= x1 && rec.bin1() < x2 && rec.bin2() >= y1 && rec.bin2() < y2)) && rec.counts() > countsThreshold) { if (normVector == null) { contactRecords.add(rec); } else { diff --git a/src/test/java/org/igv/hic/HicFileTest.java b/src/test/java/org/igv/hic/HicFileTest.java index 94bcd5ce32..051a73037a 100644 --- a/src/test/java/org/igv/hic/HicFileTest.java +++ b/src/test/java/org/igv/hic/HicFileTest.java @@ -33,7 +33,7 @@ public void getContactRecords() throws IOException { Region region1 = new Region("chr1", 0, 249000000); Region region2 = new Region("chr1", 0, 249000000); int binSize = 250000; - List records = hicFile.getContactRecords(region1, region2, "BP", binSize, "NONE", true); + List records = hicFile.getContactRecords(region1, region2, "BP", binSize, "NONE", true, 0); assertEquals(396963, records.size()); }