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Hi, is anyone sure if the ribosome fasta file created is hg19 or hg38?
  • UNSWGiiGii
  • 1
  • Opened 
    on Dec 7, 2022
  • #9

Hi, Is anyone aware if the ribosome fasta file created is hg19 or hg38?
  • UNSWGiiGii
  • Opened 
    on Dec 7, 2022
  • #8

Hi Igor, small thing - in the calculate_cluster_expression function it throws this error: Error in rownames_to_column(., gene ) : is.data.frame(df) is not TRUE Calls: calculate_cluster_expression - ...
  • mcornwell1957
  • 2
  • Opened 
    on May 11, 2021
  • #7

HI, What should be in file format for adding the GFP to the cDNA file ? I am trying to add GFP ATGCCCGCCATGAAGATCGAGTGCCGCATCACCGGCACCCTGAACGGCGTGGAGTTCGAGCTGGTGGGCGGCGGAGAGGGCACCCCCGAGCAGGGCCGCATGACCAACAAGATGAA ...
  • amitpande74
  • 3
  • Opened 
    on Apr 27, 2021
  • #6

THere is no stop codon. Not sure if there is more sequence following the AAG, or there should be a stop. But just wanted to let you know.
  • niko-balanis
  • 4
  • Opened 
    on Nov 6, 2019
  • #4

Hi I used the given method to create the ribosomal RNA reference sequence using Rfam 14.1 for humans (species= homo_sapiens ). After following all your steps I am left with 0 rRNAs in order to proceed ...
  • biobug16
  • 1
  • Opened 
    on Aug 28, 2019
  • #3
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