diff --git a/workflows/amplicon/dada2/dada2_paired-tests.yml b/workflows/amplicon/dada2/dada2_paired-tests.yml
index 50d9c177d2..2d94b8cb3b 100644
--- a/workflows/amplicon/dada2/dada2_paired-tests.yml
+++ b/workflows/amplicon/dada2/dada2_paired-tests.yml
@@ -84,3 +84,87 @@
             n: 7
         - has_n_lines:
             n: 171
+- doc: Same without specifying trunction length
+  job:
+    Paired input data:
+      class: Collection
+      collection_type: list:paired
+      elements:
+      - class: Collection
+        type: paired
+        identifier: F3D0
+        elements:
+        - class: File
+          identifier: forward
+          location: https://zenodo.org/record/800651/files/F3D0_R1.fastq
+        - class: File
+          identifier: reverse
+          location: https://zenodo.org/record/800651/files/F3D0_R2.fastq
+      - class: Collection
+        type: paired
+        identifier: F3D5
+        elements:
+        - class: File
+          identifier: forward
+          location: https://zenodo.org/record/800651/files/F3D5_R1.fastq
+        - class: File
+          identifier: reverse
+          location: https://zenodo.org/record/800651/files/F3D5_R2.fastq
+      - class: Collection
+        type: paired
+        identifier: F3D145
+        elements:
+        - class: File
+          identifier: forward
+          location: https://zenodo.org/record/800651/files/F3D145_R1.fastq
+        - class: File
+          identifier: reverse
+          location: https://zenodo.org/record/800651/files/F3D145_R2.fastq
+      - class: Collection
+        type: paired
+        identifier: F3D150
+        elements:
+        - class: File
+          identifier: forward
+          location: https://zenodo.org/record/800651/files/F3D150_R1.fastq
+        - class: File
+          identifier: reverse
+          location: https://zenodo.org/record/800651/files/F3D150_R2.fastq
+      - class: Collection
+        type: paired
+        identifier: Mock
+        elements:
+        - class: File
+          identifier: forward
+          location: https://zenodo.org/record/800651/files/Mock_R1.fastq
+        - class: File
+          identifier: reverse
+          location: https://zenodo.org/record/800651/files/Mock_R2.fastq
+    Pool samples: 'FALSE'
+    Cached reference database: silva_132
+  outputs:
+    Sequence Table:
+      path: test-data/Sequence Table.dada2_sequencetable
+      asserts:
+        - has_n_columns:
+            n: 6
+        - has_n_lines:
+            n: 171
+    Counts:
+      path: test-data/Counts.tabular
+      asserts:
+        - has_n_columns:
+            n: 8
+        - has_n_lines:
+            n: 6
+    Taxonomy:
+      ftype: tabular
+      sorted: true
+      asserts:
+        - has_text:
+            text: Firmicutes
+            n: 131
+        - has_n_columns:
+            n: 7
+        - has_n_lines:
+            n: 171
\ No newline at end of file