diff --git a/tests/supervisors/test_reload.py b/tests/supervisors/test_reload.py
index 4f3190117..cc5cf7d4f 100644
--- a/tests/supervisors/test_reload.py
+++ b/tests/supervisors/test_reload.py
@@ -279,6 +279,26 @@ def test_watchfiles_no_changes(self) -> None:
 
             reloader.shutdown()
 
+    @pytest.mark.skipif(WatchFilesReload is None, reason="watchfiles not available")
+    @pytest.mark.parametrize("reloader_class", [WatchFilesReload])
+    def test_watchfiles_should_only_call_watch_filter_once_per_reload(
+        self, mocker, touch_soon
+    ):
+        mock_call = mocker.patch(
+            "uvicorn.supervisors.watchfilesreload.FileFilter.__call__"
+        )
+        file = self.reload_path / "main.py"
+
+        with as_cwd(self.reload_path):
+            config = Config(app="tests.test_config:asgi_app", reload=True)
+            reloader = self._setup_reloader(config)
+
+            self._reload_tester(touch_soon, reloader, file)
+
+            mock_call.assert_called_once()
+
+            reloader.shutdown()
+
     @pytest.mark.parametrize("reloader_class", [WatchGodReload])
     def test_should_detect_new_reload_dirs(
         self, touch_soon, caplog: pytest.LogCaptureFixture, tmp_path: Path
diff --git a/uvicorn/supervisors/watchfilesreload.py b/uvicorn/supervisors/watchfilesreload.py
index 9ba10ce9e..796bd5048 100644
--- a/uvicorn/supervisors/watchfilesreload.py
+++ b/uvicorn/supervisors/watchfilesreload.py
@@ -2,7 +2,7 @@
 from socket import socket
 from typing import Callable, List, Optional
 
-from watchfiles import watch
+from watchfiles import Change, watch
 
 from uvicorn.config import Config
 from uvicorn.supervisors.basereload import BaseReload
@@ -40,7 +40,8 @@ def __init__(self, config: Config):
                 self.excludes.append(e)
         self.excludes = list(set(self.excludes))
 
-    def __call__(self, path: Path) -> bool:
+    def __call__(self, change: Change, path_string: str) -> bool:
+        path = Path(path_string)
         for include_pattern in self.includes:
             if path.match(include_pattern):
                 for exclude_dir in self.exclude_dirs:
@@ -74,7 +75,7 @@ def __init__(
         self.watch_filter = FileFilter(config)
         self.watcher = watch(
             *self.reload_dirs,
-            watch_filter=None,
+            watch_filter=self.watch_filter,
             stop_event=self.should_exit,
             # using yield_on_timeout here mostly to make sure tests don't
             # hang forever, won't affect the class's behavior
@@ -85,5 +86,5 @@ def should_restart(self) -> Optional[List[Path]]:
         changes = next(self.watcher)
         if changes:
             unique_paths = {Path(c[1]) for c in changes}
-            return [p for p in unique_paths if self.watch_filter(p)]
+            return [p for p in unique_paths]
         return None