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Not sure if this is a bowtie issue or bison, but the aligned reads from bison do not seem to be properly sorted by read ID. For example, here are 20 read IDs from a file I've been aligning using bison:
Not sure if this is a bowtie issue or bison, but the aligned reads from bison do not seem to be properly sorted by read ID. For example, here are 20 read IDs from a file I've been aligning using bison:
Some programs like Samblaster, expect the aligned reads to be sorted so that "SRR892990.1.2" is located immediately after "SRR892990.1.1".
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