Wondering why many sharp peaks created in the deeptools 2.0 heatmap profile

[zx0223winner]

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computeMatrix reference-point
--referencePoint TSS
-b 3000
-a 3000
-R {input.bed}
-S {input.bigwig}
--binSize 50
-o {output}
--outFileSortedRegions {params.dir1} \

plotHeatmap
-m {input}
-out {output}
--interpolationMethod auto
--plotTitle {params}
--colorList 'yellow, red' 'green, blue' \

plotProfile
-m {input}
-out {output}
--plotTitle {params} \

[zx0223winner]

@WardDeb

[ckuenne]

just guessing, but maybe you have regions with extreme outlier bins that drive the average up. try to just plot the top50 regions.