Question: Can this pipeline be used for bacterial methylation detectin.

[martinastoycheva]

Hello,

In E.coli Dcm methyltransferases methylate the C5 position of the second cytosine in the sequences CCAGG and CCTGG. I was wondering if I could use your tool for detecting this in bacteria or is it somehow restricted to the CpG sites in eukaryotes?

Thank you!
Martina

[EduEyras]

Hi Martina, We decided to focus on CpG sites, which are the most common methylation sites in humans, but everything should be applicable to non-CG sites. However, we have not compared non-CG site predictions with bisulfite data. best Eduardo

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On Tue, 21 Jun 2022 at 22:37, Martina Stoycheva ***@***.***> wrote: Hello, In E.coli Dcm methyltransferases methylate the C5 position of the second cytosine in the sequences CCAGG and CCTGG. I was wondering if I could use your tool for detecting this in bacteria or is it somehow restricted to the CpG sites in eukaryotes? Thank you! Martina — Reply to this email directly, view it on GitHub <#31>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ADCZKB3WBCKLDUQVIPGE2VLVQGZPXANCNFSM5ZMGCDDA> . You are receiving this because you are subscribed to this thread.Message ID: ***@***.***>

-- Prof. E Eyras EMBL Australia Group Leader The John Curtin School of Medical Research - Australian National University https://github.com/comprna http://scholar.google.com/citations?user=LiojlGoAAAAJ