Can not connect EBB36

[MibuchiYuta]

I have connected the IO2CAN to the Raspberry Pi Zero 2 W, and connected the SKR PICO and Zero 2 W via UART, as well as the IO2CAN and EBB36. This is the configuration for the Voron 0.

The Zero 2W has Mainsail OS (64-bit) installed and configured.

I wrote the dtoverlay settings from this repository to the /boot/firmware/config.txt file and rebooted.
However, even after rebooting, dmesg does not indicate that CAN is functioning properly. I also reprogrammed the EBB36 firmware, but the result did not change.

:~ $ ~/klippy-env/bin/python ~/klipper/scripts/canbus_query.py can0
Total 0 uuids found
:~ $ ip -s -d link show can0
2: can0: <NOARP,UP,LOWER_UP,ECHO> mtu 16 qdisc pfifo_fast state UP mode DEFAULT group default qlen 128
    link/can  promiscuity 0  allmulti 0 minmtu 0 maxmtu 0 
    can state ERROR-PASSIVE restart-ms 0 
	  bitrate 500000 sample-point 0.833
	  tq 166 prop-seg 4 phase-seg1 5 phase-seg2 2 sjw 1 brp 2
	  mcp251x: tseg1 3..16 tseg2 2..8 sjw 1..4 brp 1..64 brp_inc 1
	  clock 12000000 
	  re-started bus-errors arbit-lost error-warn error-pass bus-off
	  0          0          0          1          1          0         numtxqueues 1 numrxqueues 1 gso_max_size 65536 gso_max_segs 65535 tso_max_size 65536 tso_max_segs 65535 gro_max_size 65536 parentbus spi parentdev spi0.0 
    RX:  bytes packets errors dropped  missed   mcast           
             0       0      0       0       0       0 
    TX:  bytes packets errors dropped carrier collsns           
             0       0      0       0       0       0 
:~ $ dmesg | grep -i '\(can\|spi\)'
[    7.660692] CAN device driver interface
[    7.727552] mcp251x spi0.0 can0: MCP2515 successfully initialized.
[    8.173126] vc4-drm soc:gpu: [drm] Cannot find any crtc or sizes
[    8.174083] vc4-drm soc:gpu: [drm] Cannot find any crtc or sizes
[   68.433064] can: controller area network core
[   68.433164] NET: Registered PF_CAN protocol family
[   68.453103] can: raw protocol