diff --git a/docs/404.html b/docs/404.html new file mode 100644 index 0000000..70bca70 --- /dev/null +++ b/docs/404.html @@ -0,0 +1,110 @@ + + +
+ + + + +Version 3, 29 June 2007
Copyright © 2007 Free Software Foundation, Inc. <http://fsf.org/>
Everyone is permitted to copy and distribute verbatim copies of this license document, but changing it is not allowed.
+The GNU General Public License is a free, copyleft license for software and other kinds of works.
+The licenses for most software and other practical works are designed to take away your freedom to share and change the works. By contrast, the GNU General Public License is intended to guarantee your freedom to share and change all versions of a program–to make sure it remains free software for all its users. We, the Free Software Foundation, use the GNU General Public License for most of our software; it applies also to any other work released this way by its authors. You can apply it to your programs, too.
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+If you convey a covered work, knowingly relying on a patent license, and the Corresponding Source of the work is not available for anyone to copy, free of charge and under the terms of this License, through a publicly available network server or other readily accessible means, then you must either (1) cause the Corresponding Source to be so available, or (2) arrange to deprive yourself of the benefit of the patent license for this particular work, or (3) arrange, in a manner consistent with the requirements of this License, to extend the patent license to downstream recipients. “Knowingly relying” means you have actual knowledge that, but for the patent license, your conveying the covered work in a country, or your recipient’s use of the covered work in a country, would infringe one or more identifiable patents in that country that you have reason to believe are valid.
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+A patent license is “discriminatory” if it does not include within the scope of its coverage, prohibits the exercise of, or is conditioned on the non-exercise of one or more of the rights that are specifically granted under this License. You may not convey a covered work if you are a party to an arrangement with a third party that is in the business of distributing software, under which you make payment to the third party based on the extent of your activity of conveying the work, and under which the third party grants, to any of the parties who would receive the covered work from you, a discriminatory patent license (a) in connection with copies of the covered work conveyed by you (or copies made from those copies), or (b) primarily for and in connection with specific products or compilations that contain the covered work, unless you entered into that arrangement, or that patent license was granted, prior to 28 March 2007.
+Nothing in this License shall be construed as excluding or limiting any implied license or other defenses to infringement that may otherwise be available to you under applicable patent law.
+If conditions are imposed on you (whether by court order, agreement or otherwise) that contradict the conditions of this License, they do not excuse you from the conditions of this License. If you cannot convey a covered work so as to satisfy simultaneously your obligations under this License and any other pertinent obligations, then as a consequence you may not convey it at all. For example, if you agree to terms that obligate you to collect a royalty for further conveying from those to whom you convey the Program, the only way you could satisfy both those terms and this License would be to refrain entirely from conveying the Program.
+Notwithstanding any other provision of this License, you have permission to link or combine any covered work with a work licensed under version 3 of the GNU Affero General Public License into a single combined work, and to convey the resulting work. The terms of this License will continue to apply to the part which is the covered work, but the special requirements of the GNU Affero General Public License, section 13, concerning interaction through a network will apply to the combination as such.
+The Free Software Foundation may publish revised and/or new versions of the GNU General Public License from time to time. Such new versions will be similar in spirit to the present version, but may differ in detail to address new problems or concerns.
+Each version is given a distinguishing version number. If the Program specifies that a certain numbered version of the GNU General Public License “or any later version” applies to it, you have the option of following the terms and conditions either of that numbered version or of any later version published by the Free Software Foundation. If the Program does not specify a version number of the GNU General Public License, you may choose any version ever published by the Free Software Foundation.
+If the Program specifies that a proxy can decide which future versions of the GNU General Public License can be used, that proxy’s public statement of acceptance of a version permanently authorizes you to choose that version for the Program.
+Later license versions may give you additional or different permissions. However, no additional obligations are imposed on any author or copyright holder as a result of your choosing to follow a later version.
+THERE IS NO WARRANTY FOR THE PROGRAM, TO THE EXTENT PERMITTED BY APPLICABLE LAW. EXCEPT WHEN OTHERWISE STATED IN WRITING THE COPYRIGHT HOLDERS AND/OR OTHER PARTIES PROVIDE THE PROGRAM “AS IS” WITHOUT WARRANTY OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. THE ENTIRE RISK AS TO THE QUALITY AND PERFORMANCE OF THE PROGRAM IS WITH YOU. SHOULD THE PROGRAM PROVE DEFECTIVE, YOU ASSUME THE COST OF ALL NECESSARY SERVICING, REPAIR OR CORRECTION.
+IN NO EVENT UNLESS REQUIRED BY APPLICABLE LAW OR AGREED TO IN WRITING WILL ANY COPYRIGHT HOLDER, OR ANY OTHER PARTY WHO MODIFIES AND/OR CONVEYS THE PROGRAM AS PERMITTED ABOVE, BE LIABLE TO YOU FOR DAMAGES, INCLUDING ANY GENERAL, SPECIAL, INCIDENTAL OR CONSEQUENTIAL DAMAGES ARISING OUT OF THE USE OR INABILITY TO USE THE PROGRAM (INCLUDING BUT NOT LIMITED TO LOSS OF DATA OR DATA BEING RENDERED INACCURATE OR LOSSES SUSTAINED BY YOU OR THIRD PARTIES OR A FAILURE OF THE PROGRAM TO OPERATE WITH ANY OTHER PROGRAMS), EVEN IF SUCH HOLDER OR OTHER PARTY HAS BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES.
+If the disclaimer of warranty and limitation of liability provided above cannot be given local legal effect according to their terms, reviewing courts shall apply local law that most closely approximates an absolute waiver of all civil liability in connection with the Program, unless a warranty or assumption of liability accompanies a copy of the Program in return for a fee.
+END OF TERMS AND CONDITIONS
+If you develop a new program, and you want it to be of the greatest possible use to the public, the best way to achieve this is to make it free software which everyone can redistribute and change under these terms.
+To do so, attach the following notices to the program. It is safest to attach them to the start of each source file to most effectively state the exclusion of warranty; and each file should have at least the “copyright” line and a pointer to where the full notice is found.
+<one line to give the program's name and a brief idea of what it does.>
+Copyright (C) <year> <name of author>
+
+This program is free software: you can redistribute it and/or modify
+it under the terms of the GNU General Public License as published by
+the Free Software Foundation, either version 3 of the License, or
+(at your option) any later version.
+
+This program is distributed in the hope that it will be useful,
+but WITHOUT ANY WARRANTY; without even the implied warranty of
+MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
+GNU General Public License for more details.
+
+You should have received a copy of the GNU General Public License
+along with this program. If not, see <http://www.gnu.org/licenses/>.Also add information on how to contact you by electronic and paper mail.
+If the program does terminal interaction, make it output a short notice like this when it starts in an interactive mode:
+<program> Copyright (C) <year> <name of author>
+This program comes with ABSOLUTELY NO WARRANTY; for details type 'show w'.
+This is free software, and you are welcome to redistribute it
+under certain conditions; type 'show c' for details.The hypothetical commands show w and show c should show the appropriate parts of the General Public License. Of course, your program’s commands might be different; for a GUI interface, you would use an “about box”.
You should also get your employer (if you work as a programmer) or school, if any, to sign a “copyright disclaimer” for the program, if necessary. For more information on this, and how to apply and follow the GNU GPL, see <http://www.gnu.org/licenses/>.
+The GNU General Public License does not permit incorporating your program into proprietary programs. If your program is a subroutine library, you may consider it more useful to permit linking proprietary applications with the library. If this is what you want to do, use the GNU Lesser General Public License instead of this License. But first, please read <http://www.gnu.org/philosophy/why-not-lgpl.html>.
+The goal of blit is to make it easy to execute command line tool from R.
You can install blit from CRAN using:
+install.packages("blit")Alternatively, install the development version from GitHub with:
+
+# install.packages("remotes")
+remotes::install_github("WangLabCSU/blit")To build a command, simply use exec. The first argument is the command name, and you can also provide the full path. After that, pass the command parameters. This will create a command object:
+exec("echo", "$PATH")
+#> <Execute: echo>To run the command, just pass the command object to the cmd_run() (Note: stdout = "|" is always used in the vignette to ensure that the standard output can be captured by knitr.)
+Sys.setenv(TEST = "blit is awesome")
+exec("echo", "$TEST") |> cmd_run(stdout = "|")
+#> Running command (2025-04-08 05:58:19): echo $TEST
+#>
+#> blit is awesome
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeedAlternatively, you can run it in the background. In this case, a process object will be returned. For more information, refer to the official site:
+proc <- exec("echo", "$TEST") |> cmd_background(stdout = "")
+proc$kill()
+Sys.unsetenv("TEST")++ +We use some tricks to capture the output from the background process. The actual implementation in the
+README.Rmddiffers, but the output remains the same.
cmd_background() is provided for completeness. Instead of using this function, we recommend using cmd_parallel(), which can run multiple commands in the background while ensuring that all processes are properly cleaned up when the process exits.
+# ip address are copied from quora <What are some famous IP addresses?>: https://qr.ae/pYlnbQ
+address <- c("localhost", "208.67.222.222", "8.8.8.8", "8.8.4.4")
+cmd_parallel(
+ !!!lapply(address, function(ip) exec("ping", ip)),
+ stdouts = TRUE,
+ stdout_callbacks = lapply(
+ seq_len(4),
+ function(i) {
+ force(i)
+ function(text, proc) {
+ sprintf("Connection %d: %s", i, text)
+ }
+ }
+ ),
+ timeouts = 4, # terminate after 4s
+ threads = 4
+)
+#> Running command (2025-04-08 05:58:19): ping localhost
+#> Running command (2025-04-08 05:58:19): ping 208.67.222.222
+#> Running command (2025-04-08 05:58:19): ping 8.8.8.8
+#> Running command (2025-04-08 05:58:19): ping 8.8.4.4
+#>
+#> Connection 1: PING localhost (::1) 56 data bytes
+#> Connection 1: 64 bytes from localhost (::1): icmp_seq=1 ttl=64 time=0.017 ms
+#> ⠙ 0/4 [0/s] [elapsed in 76ms] @ 2025-04-08 05:58:19
+#> ⠹ 0/4 [0/s] [elapsed in 290ms] @ 2025-04-08 05:58:20
+#> ⠸ 0/4 [0/s] [elapsed in 500ms] @ 2025-04-08 05:58:20
+#> ⠼ 0/4 [0/s] [elapsed in 710ms] @ 2025-04-08 05:58:20
+#> ⠴ 0/4 [0/s] [elapsed in 929ms] @ 2025-04-08 05:58:20
+#> ⠦ 0/4 [0/s] [elapsed in 1.1s] @ 2025-04-08 05:58:20
+#> ⠧ 0/4 [0/s] [elapsed in 1.4s] @ 2025-04-08 05:58:21
+#> ⠇ 0/4 [0/s] [elapsed in 1.6s] @ 2025-04-08 05:58:21
+#> Connection 1: 64 bytes from localhost (::1): icmp_seq=2 ttl=64 time=0.042 ms
+#> ⠏ 0/4 [0/s] [elapsed in 1.6s] @ 2025-04-08 05:58:21
+#> ⠋ 0/4 [0/s] [elapsed in 1.8s] @ 2025-04-08 05:58:21
+#> ⠙ 0/4 [0/s] [elapsed in 2s] @ 2025-04-08 05:58:21
+#> ⠹ 0/4 [0/s] [elapsed in 2.2s] @ 2025-04-08 05:58:22
+#> Connection 1: 64 bytes from localhost (::1): icmp_seq=3 ttl=64 time=0.040 ms
+#> ⠸ 0/4 [0/s] [elapsed in 2.2s] @ 2025-04-08 05:58:22
+#> ⠼ 0/4 [0/s] [elapsed in 2.4s] @ 2025-04-08 05:58:22
+#> ⠴ 0/4 [0/s] [elapsed in 2.6s] @ 2025-04-08 05:58:22
+#> ⠦ 0/4 [0/s] [elapsed in 2.8s] @ 2025-04-08 05:58:22
+#> ⠧ 0/4 [0/s] [elapsed in 3s] @ 2025-04-08 05:58:22
+#> ⠇ 0/4 [0/s] [elapsed in 3.3s] @ 2025-04-08 05:58:23
+#> ⠏ 0/4 [0/s] [elapsed in 3.5s] @ 2025-04-08 05:58:23
+#> ⠋ 0/4 [0/s] [elapsed in 3.7s] @ 2025-04-08 05:58:23
+#> Connection 1: 64 bytes from localhost (::1): icmp_seq=4 ttl=64 time=0.039 ms
+#> ⠙ 0/4 [0/s] [elapsed in 3.7s] @ 2025-04-08 05:58:23
+#> ⠹ 0/4 [0/s] [elapsed in 3.9s] @ 2025-04-08 05:58:23
+#> ⠸ 0/4 [0/s] [elapsed in 4.1s] @ 2025-04-08 05:58:23
+#> ⠼ 0/4 [0/s] [elapsed in 4.1s] @ 2025-04-08 05:58:23
+#> Running scheduled exit task
+#> Command process finished
+#> Running scheduled exit task
+#> Command process finished
+#> Running scheduled exit task
+#> Command process finished
+#> Running scheduled exit task
+#> Command process finished
+#> ⠼ 4/4 [0.96/s] [elapsed in 4.2s] @ 2025-04-08 05:58:23
+#> Warning: [Command: 1] System command timed out in 4 secs (status: -9)
+#> Warning: [Command: 2] System command timed out in 4.1 secs (status: -9)
+#> Warning: [Command: 3] System command timed out in 4.1 secs (status: -9)
+#> Warning: [Command: 4] System command timed out in 4.1 secs (status: -9)The blit package provides several functions to manage and control the environment context:
cmd_wd: define the working directory.cmd_envvar: define the environment variables.cmd_envpath: define the PATH-like environment variables.cmd_condaenv: define the PATH environment variables with conda environment.
+exec("echo", "$(pwd)") |>
+ cmd_wd(tempdir()) |>
+ cmd_run(stdout = "|")
+#> Working Directory: '/tmp/Rtmp2bxDJx'
+#> Running command (2025-04-08 05:58:24): echo $(pwd)
+#>
+#> /tmp/Rtmp2bxDJx
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeed
+exec("echo", "$TEST") |>
+ cmd_envvar(TEST = "blit is very awesome") |>
+ cmd_run(stdout = "|")
+#> Setting environment variables: TEST
+#> Running command (2025-04-08 05:58:24): echo $TEST
+#>
+#> blit is very awesome
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeed
+exec("echo", "$PATH") |>
+ cmd_envpath("PATH_IS_HERE", action = "replace") |>
+ cmd_run(stdout = "|")
+#> Setting environment variables: PATH
+#> Running command (2025-04-08 05:58:24): echo $PATH
+#>
+#> PATH_IS_HERE
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeed++Note:
+echois a built-in command of the linux shell, so it remains available even after modifying thePATHenvironment variable.
cmd_condaenv() can add conda/mamba environment prefix to the PATH environment variable.
Conda/mamba are open-source package and environment management systems that facilitate the installation of multiple software versions and their dependencies. They allow easy switching between environments and are compatible with Linux, macOS, and Windows.
cmd_condaenv() function accepts multiple conda/mamba environment prefixes and an optional root argument specifying the path to the conda/mamba root prefix. If root is not provided, the function searches for the root in the following order:
blit.conda.root.BLIT_CONDA_ROOT.appmamba()] (Please see the Software management section for details).The cmd_condaenv() function searches for the specified environment prefix within the provided root path.
The blit package integrates with micromamba, a lightweight version of the mamba package manager, for efficient software environment management.
You can install micromamba with install_appmamba().
+install_appmamba()
+#> Installing appmamba
+#> Downloading from 'https://micro.mamba.pm/api/micromamba/linux-64/latest'
+#> Install appmamba successfully!The appmamba() function executes specified micromamba commands. Running it without arguments shows the help document:
+appmamba()
+#> Running command (2025-04-08 05:58:27):
+#> /home/runner/.local/share/R/blit/apps/appmamba/bin/micromamba --root-prefix
+#> /home/runner/.local/share/R/blit/appmamba --helpTo create a new environment named samtools and install samtools from Bioconda, use:
+appmamba("create", "--yes", "--name samtools", "bioconda::samtools")
+#> Running command (2025-04-08 05:58:27):
+#> /home/runner/.local/share/R/blit/apps/appmamba/bin/micromamba --root-prefix
+#> /home/runner/.local/share/R/blit/appmamba create --yes --name samtools
+#> bioconda::samtoolsOnce the environment is created, you can execute commands within it. The following example locates the samtools binary within the specified environment:
+
+exec("which", "samtools") |>
+ cmd_condaenv("samtools") |>
+ cmd_run()
+#> Setting environment variables: PATH
+#> Running command (2025-04-08 05:58:39): which samtools
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeedYou may want to clean the created environment-samtools.
+appmamba("env", "remove", "--yes", "--name samtools")
+#> Running command (2025-04-08 05:58:39):
+#> /home/runner/.local/share/R/blit/apps/appmamba/bin/micromamba --root-prefix
+#> /home/runner/.local/share/R/blit/appmamba env remove --yes --name samtoolsFor more details, please see https://mamba.readthedocs.io/en/latest/user_guide/micromamba.html.
+Several functions allow you to schedule expressions:
+cmd_on_start/cmd_on_exit: define the startup, or exit code of the command.cmd_on_succeed/cmd_on_fail: define the code to be run when command succeed or fail.
+file <- tempfile()
+file.create(file)
+#> [1] TRUE
+file.exists(file)
+#> [1] TRUE
+exec("ping", "localhost") |>
+ cmd_on_exit(file.remove(file)) |>
+ cmd_run(timeout = 5, stdout = "|") # terminate it after 5s
+#> Running command (2025-04-08 05:58:40): ping localhost
+#>
+#> PING localhost (::1) 56 data bytes
+#> 64 bytes from localhost (::1): icmp_seq=1 ttl=64 time=0.016 ms
+#> 64 bytes from localhost (::1): icmp_seq=2 ttl=64 time=0.028 ms
+#> 64 bytes from localhost (::1): icmp_seq=3 ttl=64 time=0.029 ms
+#> 64 bytes from localhost (::1): icmp_seq=4 ttl=64 time=0.030 ms
+#> 64 bytes from localhost (::1): icmp_seq=5 ttl=64 time=0.030 ms
+#> Running scheduled exit task
+#> Command process finished
+#> Warning: System command timed out in 5 secs (status: -9)
+file.exists(file)
+#> [1] FALSEWe can also register code for succeessful or failure command respectively (Timeout means command fail):
+
+file <- tempfile()
+file.create(file)
+#> [1] TRUE
+file.exists(file)
+#> [1] TRUE
+exec("ping", "localhost") |>
+ cmd_on_fail(file.remove(file)) |>
+ cmd_run(timeout = 5, stdout = "|") # terminate it after 5s
+#> Running command (2025-04-08 05:58:45): ping localhost
+#>
+#> PING localhost (::1) 56 data bytes
+#> 64 bytes from localhost (::1): icmp_seq=1 ttl=64 time=0.017 ms
+#> 64 bytes from localhost (::1): icmp_seq=2 ttl=64 time=0.029 ms
+#> 64 bytes from localhost (::1): icmp_seq=3 ttl=64 time=0.030 ms
+#> 64 bytes from localhost (::1): icmp_seq=4 ttl=64 time=0.035 ms
+#> 64 bytes from localhost (::1): icmp_seq=5 ttl=64 time=0.027 ms
+#> Running the scheduled failed task
+#> Running scheduled exit task
+#> Command process finished
+#> Warning: System command timed out in 5 secs (status: -9)
+file.exists(file)
+#> [1] FALSE
+file <- tempfile()
+file.create(file)
+#> [1] TRUE
+file.exists(file)
+#> [1] TRUE
+exec("ping", "localhost") |>
+ cmd_on_succeed(file.remove(file)) |>
+ cmd_run(timeout = 5, stdout = "|") # terminate it after 5s
+#> Running command (2025-04-08 05:58:50): ping localhost
+#>
+#> PING localhost (::1) 56 data bytes
+#> 64 bytes from localhost (::1): icmp_seq=1 ttl=64 time=0.017 ms
+#> 64 bytes from localhost (::1): icmp_seq=2 ttl=64 time=0.029 ms
+#> 64 bytes from localhost (::1): icmp_seq=3 ttl=64 time=0.029 ms
+#> 64 bytes from localhost (::1): icmp_seq=4 ttl=64 time=0.034 ms
+#> 64 bytes from localhost (::1): icmp_seq=5 ttl=64 time=0.029 ms
+#> Running scheduled exit task
+#> Command process finished
+#> Warning: System command timed out in 5 secs (status: -9)
+file.exists(file) # file remain exist as timeout means command failed
+#> [1] TRUE
+file.remove(file)
+#> [1] TRUEblit provides several built-in functions for directly executing specific commands., these include: samtools, alleleCounter, cellranger, fastq_pair, gistic2, KrakenTools, kraken2, perl, pySCENIC, python, seqkit, trust4.
For these commands, you can also use cmd_help() to print the help document.
+python() |> cmd_help(stdout = "|")
+#> Running command (2025-04-08 05:58:55): /usr/bin/python --help
+#>
+#> usage: /usr/bin/python [option] ... [-c cmd | -m mod | file | -] [arg] ...
+#> Options (and corresponding environment variables):
+#> -b : issue warnings about converting bytes/bytearray to str and comparing
+#> bytes/bytearray with str or bytes with int. (-bb: issue errors)
+#> -B : don't write .pyc files on import; also PYTHONDONTWRITEBYTECODE=x
+#> -c cmd : program passed in as string (terminates option list)
+#> -d : turn on parser debugging output (for experts only, only works on
+#> debug builds); also PYTHONDEBUG=x
+#> -E : ignore PYTHON* environment variables (such as PYTHONPATH)
+#> -h : print this help message and exit (also -? or --help)
+#> -i : inspect interactively after running script; forces a prompt even
+#> if stdin does not appear to be a terminal; also PYTHONINSPECT=x
+#> -I : isolate Python from the user's environment (implies -E and -s)
+#> -m mod : run library module as a script (terminates option list)
+#> -O : remove assert and __debug__-dependent statements; add .opt-1 before
+#> .pyc extension; also PYTHONOPTIMIZE=x
+#> -OO : do -O changes and also discard docstrings; add .opt-2 before
+#> .pyc extension
+#> -P : don't prepend a potentially unsafe path to sys.path; also
+#> PYTHONSAFEPATH
+#> -q : don't print version and copyright messages on interactive startup
+#> -s : don't add user site directory to sys.path; also PYTHONNOUSERSITE=x
+#> -S : don't imply 'import site' on initialization
+#> -u : force the stdout and stderr streams to be unbuffered;
+#> this option has no effect on stdin; also PYTHONUNBUFFERED=x
+#> -v : verbose (trace import statements); also PYTHONVERBOSE=x
+#> can be supplied multiple times to increase verbosity
+#> -V : print the Python version number and exit (also --version)
+#> when given twice, print more information about the build
+#> -W arg : warning control; arg is action:message:category:module:lineno
+#> also PYTHONWARNINGS=arg
+#> -x : skip first line of source, allowing use of non-Unix forms of #!cmd
+#> -X opt : set implementation-specific option
+#> --check-hash-based-pycs always|default|never:
+#> control how Python invalidates hash-based .pyc files
+#> --help-env: print help about Python environment variables and exit
+#> --help-xoptions: print help about implementation-specific -X options and exit
+#> --help-all: print complete help information and exit
+#>
+#> Arguments:
+#> file : program read from script file
+#> - : program read from stdin (default; interactive mode if a tty)
+#> arg ...: arguments passed to program in sys.argv[1:]
+#> Running scheduled exit task
+#> Command process finished
+perl() |> cmd_help(stdout = "|")
+#> Running command (2025-04-08 05:58:55): /usr/bin/perl --help
+#>
+#>
+#> Usage: /usr/bin/perl [switches] [--] [programfile] [arguments]
+#> -0[octal/hexadecimal] specify record separator (\0, if no argument)
+#> -a autosplit mode with -n or -p (splits $_ into @F)
+#> -C[number/list] enables the listed Unicode features
+#> -c check syntax only (runs BEGIN and CHECK blocks)
+#> -d[t][:MOD] run program under debugger or module Devel::MOD
+#> -D[number/letters] set debugging flags (argument is a bit mask or alphabets)
+#> -e commandline one line of program (several -e's allowed, omit programfile)
+#> -E commandline like -e, but enables all optional features
+#> -f don't do $sitelib/sitecustomize.pl at startup
+#> -F/pattern/ split() pattern for -a switch (//'s are optional)
+#> -g read all input in one go (slurp), rather than line-by-line (alias for -0777)
+#> -i[extension] edit <> files in place (makes backup if extension supplied)
+#> -Idirectory specify @INC/#include directory (several -I's allowed)
+#> -l[octnum] enable line ending processing, specifies line terminator
+#> -[mM][-]module execute "use/no module..." before executing program
+#> -n assume "while (<>) { ... }" loop around program
+#> -p assume loop like -n but print line also, like sed
+#> -s enable rudimentary parsing for switches after programfile
+#> -S look for programfile using PATH environment variable
+#> -t enable tainting warnings
+#> -T enable tainting checks
+#> -u dump core after parsing program
+#> -U allow unsafe operations
+#> -v print version, patchlevel and license
+#> -V[:configvar] print configuration summary (or a single Config.pm variable)
+#> -w enable many useful warnings
+#> -W enable all warnings
+#> -x[directory] ignore text before #!perl line (optionally cd to directory)
+#> -X disable all warnings
+#>
+#> Run 'perldoc perl' for more help with Perl.
+#> Running scheduled exit task
+#> Command process finishedAnd it is very easily to extend for other commands.
+One of the great features of blit is its ability to translate the R pipe (%>% or |>) into the Linux pipe (|). All functions used to create a command object can accept another command object. The internal will capture the first unnamed input value. If it is a command object, it will be removed from the call and saved. When the command object is run, the saved command will be passed through the pipe (|) to the command. Here we take the gzip command as an example (assuming you’re using a Linux system).
+tmpdir <- tempdir()
+file <- tempfile(tmpdir = tmpdir)
+writeLines(letters, con = file)
+file2 <- tempfile()
+exec("gzip", "-c", file) |>
+ exec("gzip", "-d", ">", file2) |>
+ cmd_run(stdout = "|")
+#> Running command (2025-04-08 05:58:55): gzip -c /tmp/Rtmp2bxDJx/file1db163a56a5d
+#> | gzip -d > /tmp/Rtmp2bxDJx/file1db14f8f6795
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeed
+identical(readLines(file), readLines(file2))
+#> [1] TRUEIn the last we clean the temporary files.
+
+file.remove(file)
+#> [1] TRUE
+file.remove(file2)
+#> [1] TRUETo add a new command, use the make_command function. This helper function is designed to assist developers in creating functions that initialize new command objects. A command object is a bundle of multiple Command R6 objects (note the uppercase "C" in Command, which distinguishes it from the command object) and the associated running environment (including the working directory and environment variables).
The make_command function accepts a function that initializes a new Command object and, when necessary, validates the input arguments. The core purpose is to create a new Command R6 object, so familiarity with the R6 class system is essential.
There are several private methods or fields you may want to override when creating a new Command R6 object. The first method is command_locate, which determines how to locate the command path. By default, it will attempt to use the cmd argument provided by the user. If no cmd argument is supplied, it will try to locate the command using the alias method. In most cases, you will only need to provide values for the alias method, rather than overriding the command_locate method.
For example, consider the ping command. Here is how you can define it:
+Ping <- R6::R6Class(
+ "Ping",
+ inherit = Command,
+ private = list(alias = function() "ping")
+)
+ping <- make_command("ping", function(..., ping = NULL) {
+ Ping$new(cmd = ping, ...)
+})
+ping("8.8.8.8") |> cmd_run(timeout = 5, stdout = "|") # terminate it after 5s
+#> Running command (2025-04-08 05:58:55): /usr/bin/ping 8.8.8.8
+#> Running scheduled exit task
+#> Command process finished
+#> Warning: System command timed out in 5 secs (status: -9)For command-line tools, the input parameters should always be characters. The core principle of the Command object is to convert all R objects (such as data frames) into characters—typically file paths of R objects that have been saved to disk.
+sessionInfo()
+#> R version 4.4.3 (2025-02-28)
+#> Platform: x86_64-pc-linux-gnu
+#> Running under: Ubuntu 24.04.2 LTS
+#>
+#> Matrix products: default
+#> BLAS: /usr/lib/x86_64-linux-gnu/openblas-pthread/libblas.so.3
+#> LAPACK: /usr/lib/x86_64-linux-gnu/openblas-pthread/libopenblasp-r0.3.26.so; LAPACK version 3.12.0
+#>
+#> locale:
+#> [1] LC_CTYPE=C.UTF-8 LC_NUMERIC=C LC_TIME=C.UTF-8
+#> [4] LC_COLLATE=C.UTF-8 LC_MONETARY=C.UTF-8 LC_MESSAGES=C.UTF-8
+#> [7] LC_PAPER=C.UTF-8 LC_NAME=C LC_ADDRESS=C
+#> [10] LC_TELEPHONE=C LC_MEASUREMENT=C.UTF-8 LC_IDENTIFICATION=C
+#>
+#> time zone: UTC
+#> tzcode source: system (glibc)
+#>
+#> attached base packages:
+#> [1] stats graphics grDevices utils datasets methods base
+#>
+#> other attached packages:
+#> [1] blit_0.2.0.9000
+#>
+#> loaded via a namespace (and not attached):
+#> [1] digest_0.6.37 R6_2.6.1 fastmap_1.2.0 xfun_0.52
+#> [5] knitr_1.50 parallel_4.4.3 htmltools_0.5.8.1 rmarkdown_2.29
+#> [9] ps_1.9.0 cli_3.6.4 processx_3.8.6 data.table_1.17.0
+#> [13] compiler_4.4.3 tools_4.4.3 evaluate_1.0.3 yaml_2.3.10
+#> [17] rlang_1.1.5NEWS.md
+ new function cmd_conda to define the PATH environment variables with conda environment.
new function appmamba to install software and manage Environment with micromamba.
new function cmd_on_fail to define the expression to be evaluated when the command failed.
new function cmd_on_succeedto define the expression to be evaluated when the command succeeded.
new function cmd_on_start() to define the expressions which will be run when command started
new function cmd_on_exit() to define the expressions which will be run when command finished
new command samtools
new function cmd_parallel() to run multiple commands meanwhile
use processx package to execute the command and remove the sys and withr package from dependencies
Command is an R6 class used by developers to create new command. It should
+not be used by end users.
make_command
build_command()Build the command line
+ + + + +print()Build parameters to run command.
+ + + + +The alleleCount program primarily exists to prevent code duplication
+between some other projects, specifically AscatNGS and Battenberg.
allele_counter(
+ hts_file,
+ loci_file,
+ ofile,
+ ...,
+ odir = getwd(),
+ alleleCounter = NULL
+)A string of path to sample HTS file.
A string of path to loci file.
A string of path to the output file.
<dynamic dots> Additional arguments passed to alleleCounter command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(alleleCounter()).
A string of path to the output directory.
A string of path to alleleCounter command.
A command object.
Manage Environment with micromamba
appmamba(...)
+
+install_appmamba(force = FALSE)
+
+uninstall_appmamba()
+
+appmamba_rc(edit = FALSE)<dynamic dots> Additional arguments passed to
+micromamba. Run appmamba() for more details.
A logical value indicating whether to reinstall appmamba
+if it is already installed.
A logical value indicating whether to open the config file for +editing.
appmamba(): blit utilizes micromamba to manage environments.
+This function simply executes the specified micromamba command.
install_appmamba(): Install appmamba (micromamba).
uninstall_appmamba(): Remove appmamba (micromamba).
appmamba_rc(): Get the run commands config file of the micromamba.
# \donttest{
+install_appmamba()
+#> appmamba is already installed
+appmamba()
+#> Running command (2025-09-19 00:06:50):
+#> /home/dingjia/.local/share/R/blit/apps/appmamba/bin/micromamba --root-prefix
+#> /home/dingjia/.local/share/R/blit/appmamba --help
+#>
+appmamba("env", "list")
+#> Running command (2025-09-19 00:06:50):
+#> /home/dingjia/.local/share/R/blit/apps/appmamba/bin/micromamba --root-prefix
+#> /home/dingjia/.local/share/R/blit/appmamba env list
+#>
+# uninstall_appmamba() # Uninstall the `micromamba`
+# }
+arg() is intended for user use, while arg0() is for developers and does
+not perform argument validation.
arg(tag, value, indicator = FALSE, lgl2int = FALSE, format = "%s", sep = " ")
+
+arg0(
+ tag,
+ value,
+ indicator = FALSE,
+ lgl2int = FALSE,
+ format = "%s",
+ sep = " ",
+ allow_null = FALSE,
+ arg = caller_arg(value),
+ call = caller_call()
+)A string specifying argument tag, like "-i", "-o".
Value passed to the argument.
A logical value specifying whether value should be an
+indicator of tag. If TRUE, logical value will explain the set or unset of
+tag.
A logical value indicates whether transfrom value TRUE to
+1 or FALSE to 0. If TRUE, format will always be set to "%d".
The format of the value, details see sprintf.
A character string used to separate "tag" and "value", usually
+" " or "=".
A single logical value indicates whether value can be
+NULL.
An argument name as a string. This argument will be mentioned in +error messages as the input that is at the origin of a problem.
The execution environment of a currently running function.
A string.
+R/cmd-bcftools.R
+ bcftools.RdBCFtools is a program for variant calling and manipulating files +in the Variant Call Format (VCF) and its binary counterpart BCF. +All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed.
+bcftools(subcmd = NULL, ..., bcftools = NULL)Sub-Command of bcftools. Details see: cmd_help(bcftools()).
<dynamic dots> Additional arguments passed to bcftools command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(bcftools()).
A string of path to bcftools command.
A command object.
Other commands:
+allele_counter(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4()
An all-encompassing R toolkit designed to streamline the process of calling various bioinformatics software and then performing data analysis and visualization in R. With 'blit', users can easily integrate a wide array of bioinformatics command line tools into their workflows, leveraging the power of R for sophisticated data manipulation and graphical representation.
+Useful links:
Report bugs at https://github.com/WangLabCSU/blit/issues
Bowtie 2 is an ultrafast and memory-efficient tool +for aligning sequencing reads to long reference sequences. +It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, +and particularly good at aligning to relatively long (e.g. mammalian) genomes. +Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: +for the human genome, its memory footprint is typically around 3.2 GB. +Bowtie 2 supports gapped, local, and paired-end alignment modes.
+bowtie2(index, reads, ofile, ..., bowtie2 = NULL)Path to bowtie2 index prefix (without file extensions).
A character vector of FASTQ files used as input to bowtie2.
A string of path to the output sam file.
<dynamic dots> Additional arguments passed to bowtie2 command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(bowtie2()).
A string of path to bowtie2 command.
A command object.
Other commands:
+allele_counter(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4()
Run cellranger
+cellranger(subcmd = NULL, ..., cellranger = NULL)Sub-Command of cellranger.
<dynamic dots> Additional arguments passed to cellranger command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(cellranger()).
A string of path to cellranger command.
A command object.
https://www.10xgenomics.com/support/software/cell-ranger/latest
https://www.10xgenomics.com/support/software/cell-ranger/downloads#reference-downloads
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
if (FALSE) { # \dontrun{
+fastq_dir # 10x raw fastq files directory
+genome_ref # Please download the transcriptome reference data
+cellranger(
+ "count",
+ sprintf("--fastqs=%s", fastq_dir),
+ sprintf("--id=%s", basename(fastq_dir)),
+ sprintf("--sample=%s", basename(fastq_dir)),
+ sprintf("--localcores=%s", parallel::detectCores()),
+ sprintf("--transcriptome=%s", genome_ref),
+ sprintf("--chemistry=%s", shQuote("auto")),
+ "--nosecondary"
+)
+} # }
+conda-like environment prefix to the PATH environment variablesR/cmd-setup.R
+ cmd_conda.Rdcmd_conda(...)Additional arguments passed to cmd_condaenv().
Schedule expressions to run
+cmd_on_start(command, ...)
+
+cmd_on_exit(command, ...)
+
+cmd_on_fail(command, ...)
+
+cmd_on_succeed(command, ...)A command object.
The expressions input will be captured with
+enquos(). If your expressions depend on global data, you
+may want to unquote objects with !! to prevent
+unintended changes due to delayed evaluation.
cmd_on_start: Expression to be evaluated when the command started.
cmd_on_exit: Expression to be evaluated when the command finished.
cmd_on_fail: Expression to be evaluated when the command failed.
cmd_on_succeed: Expression to be evaluated when the command succeeded.
cmd_on_start: The command object itself, with the start code updated.
cmd_on_exit: The command object itself, with the exit code updated.
cmd_on_fail: The command object itself, with the failure code updated.
cmd_on_succeed: The command object itself, with the successful code
+updated.
cmd_on_start(): define the startup code of the command
cmd_on_exit(): define the exit code of the command
cmd_on_fail(): define the failure code of the command
cmd_on_succeed(): define the successful code of the command
Execute a list of commands
+cmd_parallel(
+ ...,
+ stdouts = FALSE,
+ stderrs = FALSE,
+ stdins = NULL,
+ stdout_callbacks = NULL,
+ stderr_callbacks = NULL,
+ timeouts = NULL,
+ threads = NULL,
+ verbose = TRUE
+)A list of command object.
Specifies how the output/error streams of the child +process are handled. One of or a list of following values:
FALSE/NULL: Suppresses the output/error stream.
TRUE: Prints the child process output/error to the R console. If a
+standard output/error stream exists, "" is used; otherwise, "|" is
+used.
string: An empty string "" inherits the standard output/error stream
+from the main R process (Printing in the R console). If the main R process
+lacks a standard output/error stream, such as in RGui on Windows, an
+error is thrown. A string "|" prints to the standard output connection
+of R process (Using cat()). Alternative, a file name or path to
+redirect the output/error. If a relative path is specified, it remains
+relative to the current working directory, even if a different directory
+is set using cmd_wd().
connection: A writable R connection object. If the connection is not
+open(), it will be automatically opened.
For stderrs, use string "2>&1" to redirect it to the same connection
+(i.e. pipe or file) as stdout.
When a single file path is specified, the stdout/stderr of all commands will +be merged into this single file.
should the input be diverted? One of or a list of following +values:
FALSE/NULL: no standard input.
TRUE: If a standard input stream exists, "" is used; otherwise, NULL
+is used.
string: An empty string "" inherits the standard input stream from
+the main R process. If the main R process lacks a standard input stream,
+such as in RGui on Windows, an error is thrown. Alternative, a file name
+or path to redirect the input. If a relative path is specified, it remains
+relative to the current working directory, even if a different directory
+is set using cmd_wd().
One of or a list of following +values:
NULL: no callback function.
function: A function invoked for each line of standard output or error.
+Non-text (non-character) output will be ignored. The function should accept
+two arguments: one for the standard output or error and another for the
+running process object.
Timeout in seconds. Can be a single value or a list, +specifying the maximum elapsed time for running the command in the separate +process.
Number of threads to use.
A single boolean value indicating whether the command +execution should be verbose.
A list of exit status invisiblely.
+cmd_parallel()
cmd_run: Run the command.
cmd_help: Print the help document for this command.
cmd_background: Run the command in the background. This function is
+provided for completeness. Instead of using this function, we recommend
+using cmd_parallel(), which can run multiple commands in the background
+while ensuring that all processes are properly cleaned up when the process
+exits.
cmd_run(
+ command,
+ stdout = TRUE,
+ stderr = TRUE,
+ stdin = TRUE,
+ stdout_callback = NULL,
+ stderr_callback = NULL,
+ timeout = NULL,
+ spinner = FALSE,
+ verbose = TRUE
+)
+
+cmd_help(
+ command,
+ stdout = TRUE,
+ stderr = TRUE,
+ stdout_callback = NULL,
+ stderr_callback = NULL,
+ verbose = TRUE
+)
+
+cmd_background(
+ command,
+ stdout = FALSE,
+ stderr = FALSE,
+ stdin = NULL,
+ verbose = TRUE
+)A command object.
Specifies how the output/error streams of the child +process are handled. Possible values include:
FALSE/NULL: Suppresses the output/error stream.
TRUE: Prints the child process output/error to the R console. If a
+standard output/error stream exists, "" is used; otherwise, "|" is
+used.
string: An empty string "" inherits the standard output/error stream
+from the main R process (Printing in the R console). If the main R process
+lacks a standard output/error stream, such as in RGui on Windows, an
+error is thrown. A string "|" prints to the standard output connection
+of R process (Using cat()). Alternative, a file name or path to
+redirect the output/error. If a relative path is specified, it remains
+relative to the current working directory, even if a different directory
+is set using cmd_wd().
connection: A writable R connection object. If the connection is not
+open(), it will be automatically opened.
For stderr, use string "2>&1" to redirect it to the same connection (i.e.
+pipe or file) as stdout.
For cmd_help(), use FALSE/NULL will do nothing, since it always want to
+display the help document.
For cmd_background(), connection cannot be used, and TRUE and "|"
+will fallback to the empty string "".
When using a connection (if not already open) or a string, wrapping it
+with I() prevents overwriting existing content.
should the input be diverted? Possible values include:
FALSE/NULL: no standard input.
TRUE: If a standard input stream exists, "" is used; otherwise, NULL
+is used.
string: An empty string "" inherits the standard input stream from
+the main R process. If the main R process lacks a standard input stream,
+such as in RGui on Windows, an error is thrown. Alternative, a file name
+or path to redirect the input. If a relative path is specified, it remains
+relative to the current working directory, even if a different directory
+is set using cmd_wd().
Possible values include:
NULL: no callback function.
function: A function invoked for each line of standard output or error.
+Non-text (non-character) output will be ignored. The function should accept
+two arguments: one for the standard output or error and another for the
+running process object.
Timeout in seconds. This is a limit for the elapsed time +running command in the separate process.
Whether to show a reassuring spinner while the process +is running.
A single boolean value indicating whether the command +execution should be verbose.
cmd_run: Exit status invisiblely.
cmd_help: The input command invisiblely.
cmd_background: A process object.
Setup the context for the command
+cmd_wd(command, wd = NULL)
+
+cmd_envvar(command, ..., action = "replace", sep = NULL)
+
+cmd_envpath(command, ..., action = "prefix", name = "PATH")
+
+cmd_condaenv(command, ..., root = NULL, action = "prefix")A command object.
A string or NULL define the working directory of the command.
<dynamic dots>:
cmd_envvar: Named character define the environment variables.
cmd_envpath: Unnamed character to define the PATH-like environment
+variables name.
cmd_condaenv: Unnamed character to specify the name of conda
+environment.
Should the new values "replace", "prefix" or "suffix"
+existing environment variables?
A string to separate new and old value when action is "prefix"
+or "suffix". By default, " " will be used.
A string define the PATH environment variable name. You
+can use this to define other PATH-like environment variable such as
+PYTHONPATH.
A string specifying the path to the conda root prefix. If not +provided, the function searches for the root in the following order:
the option blit.conda.root.
the environment variable BLIT_CONDA_ROOT.
the root prefix of appmamba().
cmd_wd: The command object itself, with working directory updated.
cmd_envvar: The command object itself, with running environment
+variable updated.
cmd_envpath: The command object itself, with running environment
+variable specified in name updated.
cmd_condaenv: The command object itself, with running environment
+variable PATH updated.
cmd_wd(): define the working directory.
cmd_envvar(): define the environment variables.
cmd_envpath(): define the PATH-like environment variables.
cmd_condaenv(): Set conda-like environment prefix to the PATH
+environment variables.
Run conda
+conda(subcmd = NULL, ..., conda = NULL)Sub-Command of conda.
<dynamic dots> Additional arguments passed to conda command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(conda()).
A string of path to conda command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
Invoke a System Command
+exec(cmd, ...)Command to be invoked, as a character string.
<dynamic dots> Additional arguments passed to cmd command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote().
A command object.
command collectionscmd_run(exec("echo", "$PATH"))
+#> Running command (2025-09-19 00:06:54): echo $PATH
+#>
+#> Running scheduled exit task
+#> Command process finished
+#> System command succeed
+The fastp is a tool designed to provide ultrafast all-in-one preprocessing
+and quality control for FastQ data.
fastp(fq1, ofile1, ..., fq2 = NULL, ofile2 = NULL, fastp = NULL)A string of fastq file path.
A string of path to the output fastq file.
<dynamic dots> Additional arguments passed to fastp command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(fastp()).
A string of path to fastp command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
Rewrite paired end fastq files to make sure that all reads have a mate and to +separate out singletons.
+Usually when you get paired end read files you have two files with a /1 +sequence in one and a /2 sequence in the other (or a /f and /r or just two +reads with the same ID). However, often when working with files from a third +party source (e.g. the SRA) there are different numbers of reads in each file +(because some reads fail QC). Spades, bowtie2 and other tools break because +they demand paired end files have the same number of reads.
+fastq_pair(
+ fq1,
+ fq2,
+ ...,
+ hash_table_size = NULL,
+ max_hash_table_size = NULL,
+ fastq_pair = NULL
+)
+
+fastq_read_pair(fastq_files)A string of fastq file path.
<dynamic dots> Additional arguments passed to fastq_pair command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(fastq_pair()).
Size of hash table to use.
Maximal hash table size to use.
A string of path to fastq_pair command.
A character of the fastq file paths.
A command object.
The GISTIC module identifies regions of the genome that are +significantly amplified or deleted across a set of samples. Each aberration +is assigned a G-score that considers the amplitude of the aberration as well +as the frequency of its occurrence across samples. False Discovery Rate +q-values are then calculated for the aberrant regions, and regions with +q-values below a user-defined threshold are considered significant. For each +significant region, a "peak region" is identified, which is the part of the +aberrant region with greatest amplitude and frequency of alteration. In +addition, a "wide peak" is determined using a leave-one-out algorithm to +allow for errors in the boundaries in a single sample. The "wide peak" +boundaries are more robust for identifying the most likely gene targets in +the region. Each significantly aberrant region is also tested to determine +whether it results primarily from broad events (longer than half a chromosome +arm), focal events, or significant levels of both. The GISTIC module reports +the genomic locations and calculated q-values for the aberrant regions. It +identifies the samples that exhibit each significant amplification or +deletion, and it lists genes found in each "wide peak" region.
+gistic2(seg, refgene, ..., odir = getwd(), gistic2 = NULL)A data.frame of segmented data.
Path to reference genome data input file (REQUIRED, see below +for file description).
<dynamic dots> Additional arguments passed to gistic2 command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(gistic2()).
A string of path to the output directory.
A string of path to gistic2 command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
+ All functions+ + |
+ |
|---|---|
| + + | +R6 Class to prepare command parameters. |
+
| + + | +Run alleleCount |
+
|
+
|
+ Manage Environment with |
+
| + + | +Deliver arguments of command |
+
| + + | +BCFtools is a program for variant calling and manipulating files in the Variant Call Format (VCF) and its binary counterpart BCF. All commands work transparently with both VCFs and BCFs, both uncompressed and BGZF-compressed. |
+
| + + | +Run bowtie2 |
+
| + + | +Run cellranger |
+
| + + | +Set |
+
| + + | +Schedule expressions to run |
+
| + + | +Execute a list of commands |
+
| + + | +Execute command |
+
| + + | +Setup the context for the command |
+
| + + | +Run conda |
+
| + + | +Invoke a System Command |
+
| + + | +Run fastp |
+
| + + | +FASTQ PAIR |
+
| + + | +Run GISTIC2 |
+
| + + | +Running Kraken2 |
+
| + + | +KrakenTools is a suite of scripts to be used alongside the Kraken, KrakenUniq, Kraken 2, or Bracken programs. |
+
| + + | +Helper function to create new command. |
+
| + + | +Perl is a highly capable, feature-rich programming language with over 36 years of development. |
+
| + + | +Run pyscenic |
+
| + + | +Python is a programming language that lets you work quickly and integrate systems more effectively. |
+
| + + | +Python is a programming language that lets you work quickly and integrate systems more effectively. |
+
| + + | +Run seqkit |
+
| + + | +TRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq data |
+
| + + | +VarScan is a platform-independent software tool developed at the Genome Institute at Washington University to detect variants in NGS data. |
+
Kraken is a taxonomic sequence classifier that assigns taxonomic labels to +DNA sequences. Kraken examines the k-mers within a query sequence and uses +the information within those k-mers to query a database. That database maps +k-mers to the lowest common ancestor (LCA) of all genomes known to contain a +given k-mer.
+kraken2(
+ reads,
+ ...,
+ ofile = "kraken_output.txt",
+ report = "kraken_report.txt",
+ classified_out = NULL,
+ unclassified_out = NULL,
+ odir = getwd(),
+ kraken2 = NULL
+)A character vector of FASTQ files used as input to Kraken2. +Can be one file (single-end) or two files (paired-end).
<dynamic dots> Additional arguments passed to kraken2 command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(kraken2()).
A string of path to save kraken2 output.
A string of path to save kraken2 report.
A string of path to save classified sequences, which +should be a fastq file.
A string of path to save unclassified sequences, +which should be a fastq file.
A string of path to the output directory.
A string of path to kraken2 command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
R/cmd-kraken2-tools.R
+ kraken_tools.RdThese scripts are designed to help Kraken users with downstream +analysis of Kraken results.
+kraken_tools(script, ..., python = NULL)Name of the kraken2 script. One of
+"combine_kreports", "combine_mpa", "extract_kraken_reads", "filter_bracken_out", "fix_unmapped", "kreport2krona", "kreport2mpa", "make_kreport", and "make_ktaxonomy".
<dynamic dots> Additional arguments passed to kraken_tools command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(kraken_tools()).
A string of path to python command.
A command object.
make_command is a helper function used by developers to create function for
+a new Command object. It should not be used by end users.
make_command(name, fun, envir = caller_env())A string of the function name.
A function used to initialize the Command object.
A environment used to bind the created function.
A function.
+R/cmd-perl.R
+ perl.RdPerl is a highly capable, feature-rich programming language with over 36 +years of development.
+perl(..., perl = NULL)<dynamic dots> Additional arguments passed to perl command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(perl()).
A string of path to perl command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
Run pyscenic
+pyscenic(subcmd = NULL, ..., pyscenic = NULL)Sub-Command of pyscenic.
<dynamic dots> Additional arguments passed to pyscenic subcmd command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(pyscenic subcmd()).
A string of path to pyscenic command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+python(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
R/cmd-python.R
+ python.RdPython is a programming language that lets you work quickly and integrate +systems more effectively.
+python(..., python = NULL)<dynamic dots> Additional arguments passed to python command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(python()).
A string of path to python command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+samtools(),
+seqkit(),
+trust4(),
+varscan()
R/cmd-samtools.R
+ samtools.RdPython is a programming language that lets you work quickly and integrate +systems more effectively.
+samtools(subcmd = NULL, ..., samtools = NULL)Sub-Command of samtools. Details see: cmd_help(samtools()).
<dynamic dots> Additional arguments passed to samtools command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(samtools()).
A string of path to samtools command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+seqkit(),
+trust4(),
+varscan()
Run seqkit
+seqkit(subcmd = NULL, ..., seqkit = NULL)Sub-Command of seqkit.
<dynamic dots> Additional arguments passed to seqkit subcmd command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(seqkit subcmd()).
A string of path to seqkit command.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+trust4(),
+varscan()
R/cmd-trust4.R
+ trust4.RdTRUST4: immune repertoire reconstruction from bulk and single-cell RNA-seq +data
+trust4(
+ file1,
+ ref_coordinate,
+ ...,
+ file2 = NULL,
+ mode = NULL,
+ ref_annot = NULL,
+ ofile = NULL,
+ odir = getwd(),
+ trust4 = NULL
+)
+
+trust4_imgt_annot(
+ species = "Homo_sapien",
+ ...,
+ ofile = "IMGT+C.fa",
+ odir = getwd(),
+ perl = NULL
+)
+
+trust4_gene_names(imgt_annot, ofile = "bcr_tcr_gene_name.txt", odir = getwd())Path to bam file or fastq file.
Path to the fasta file coordinate and sequence of +V/D/J/C genes.
trust4: <dynamic dots> Additional arguments passed to run-trust4 command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(run-trust4()).
trust4_imgt_annot: <dynamic dots> Additional arguments passed to trust4_imgt_annot command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(trust4_imgt_annot()).
Path to the second paired-end read fastq file, only used for
+mode = "fastq".
One of "bam" or "fastq". If NULL, will be inferred from
+file1.
Path to detailed V/D/J/C gene reference file, such as from +IMGT database. (default: not used). (recommended).
trust4: Prefix of output files. (default: inferred from file prefix).
trust4_imgt_annot: Output file name.
trust4_gene_names: Output file name.
A string of path to the output directory.
A string of path to run-trust4 command.
Species to extract IMGT annotation, details see +https://www.imgt.org//download/V-QUEST/IMGT_V-QUEST_reference_directory/.
A string of path to perl command.
Path of IMGT annotation file, created via
+trust4_imgt_annot.
A command object.
Other commands:
+allele_counter(),
+bowtie2(),
+bcftools(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+varscan()
R/cmd-varscan.R
+ varscan.RdVarScan is a platform-independent software tool +developed at the Genome Institute at Washington University to detect variants in NGS data.
+varscan(subcmd = NULL, ..., varscan = NULL)Sub-Command of varscan. Details see: cmd_help(varscan()).
<dynamic dots> Additional arguments passed to varscan command. Empty arguments are automatically trimmed. If a single argument, such as a file path, contains spaces, it must be quoted, for example using shQuote(). Details see: cmd_help(varscan()).
A string of path to varscan command.
A command object.
Other commands:
+allele_counter(),
+cellranger(),
+conda(),
+fastp(),
+fastq_pair(),
+gistic2(),
+kraken2(),
+kraken_tools(),
+perl(),
+pyscenic(),
+python(),
+samtools(),
+seqkit(),
+trust4()