Skip to content

Integration & annotation: consider moving cell-level annotation before integration #69

Description

@mgeaghan

Cell-level annotation doesn't need to come after integration. Additionally, the workflow could benefit from annotating cells, then assigning cell types to clusters, integrating, and then checking how well the pre-integration annotated clusters align with each other post-integration.

The new workflow would be something like:

  1. QC + doublet detection + clustering
  2. Annotation
  3. Integration
  4. Re-clustering
  5. Check alignment of pre-integration and post-integration clusters
  6. Re-assign cell types to post-integration clusters
  7. Pseudo + DE + FEA

Metadata

Metadata

Assignees

No one assigned

    Labels

    No labels
    No labels

    Type

    No type

    Fields

    No fields configured for issues without a type.

    Projects

    No projects

    Milestone

    No milestone

    Relationships

    None yet

    Development

    No branches or pull requests

    Issue actions