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PCR.py
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#!/usr/bin/env python3
from json import load
from tkinter import *
from tkinter.ttk import *
from tkinter.font import nametofont
from PIL import ImageTk
from PIL import Image
from duration import duration
from sys import exit, executable
from mix import mix
from os.path import join, dirname
if getattr(sys, "frozen", False):
datadir = dirname(executable)
else:
datadir = dirname(__file__)
with open(join(datadir, 'presets/cycle.json'), 'r') as fc:
cycle = load(fc)
with open(join(datadir, 'presets/stock.json'), 'r') as fs:
stock = load(fs)
def callback_float(p):
"""Allow only float values in entries"""
try:
p == "" or float(p)
return True
except ValueError:
return False
def callback_int(p):
"""Allow only float values in entries"""
try:
p == "" or int(p)
return True
except ValueError:
return False
class Application:
"""Define GUI elements."""
def __init__(self, master):
self.default_font = nametofont("TkDefaultFont")
self.default_font.configure(family="Arial", size=10)
Style().configure('TCheckbutton', font=('Arial', 8))
# Define stock variables and initial values
self.buffer_val = DoubleVar(value=stock['buffer'])
self.dntps_val = DoubleVar(value=stock['dntps'])
self.mgcl2_val = DoubleVar(value=stock['mgcl2'])
self.p1_val = DoubleVar(value=stock['primer1'])
self.p2_val = DoubleVar(value=stock['primer2'])
self.p3_val = DoubleVar(value=stock['primer3'])
self.p4_val = DoubleVar(value=stock['primer4'])
self.gly_val = DoubleVar(value=stock['gly'])
self.dmso_val = DoubleVar(value=stock['dmso'])
self.taq_val = DoubleVar(value=stock['taq'])
self.dnac_val = DoubleVar(value=stock['dna_c'])
# Define PRC cycle variables and initial values
self.cb_val = StringVar(value="")
self.fexten_val = DoubleVar(value=0)
self.fexten_val2 = DoubleVar(value=0)
self.id_val = DoubleVar(value=0)
self.id_val2 = DoubleVar(value=0)
self.d_val = DoubleVar(value=0)
self.d_val2 = DoubleVar(value=0)
self.anneal_val = DoubleVar(value=0)
self.anneal_val2 = DoubleVar(value=0)
self.exten_val = DoubleVar(value=0)
self.exten_val2 = DoubleVar(value=0)
self.cycle_val = DoubleVar(value=0)
self.total_time = StringVar(value='0h 00m 00s')
self.check_val = IntVar(value=0)
self.check_val_stock = IntVar(value=0)
self.check_val_pcr = IntVar(value=1)
# Define reaction variables and initial values
self.buffer_react = DoubleVar(value=1) # x (times)
self.dntps_react = DoubleVar(value=0.2) # mM - nmol/uL
self.mgcl2_react = DoubleVar(value=1.5) # mM - nmol/uL
self.primer1_react = DoubleVar(value=0.2) # uM - pmol/uL
self.primer2_react = DoubleVar(value=0.2) # uM - pmol/uL
self.primer3_react = DoubleVar(value=0) # uM - pmol/uL
self.primer4_react = DoubleVar(value=0) # uM - pmol/uL
self.glycerol_react = DoubleVar(value=0) # % (v/v)
self.dmso_react = DoubleVar(value=0) # % (v/v)
self.taq_react = DoubleVar(value=0.05) # U/reaction (units)
self.dna_mass = DoubleVar(value=5) # ng
self.dna_add = DoubleVar(value=(self.dna_mass.get() /
self.dnac_val.get())) # uL
self.volume = DoubleVar(value=12.5) # uL
self.reaction_number = IntVar(value=1)
# Trace PCR cycle values changes
self.cb_val.trace_add('write', self.locus_value)
self.id_val.trace_add('write', self.locus_value)
self.d_val.trace_add('write', self.locus_value)
self.anneal_val.trace_add('write', self.locus_value)
self.exten_val.trace_add('write', self.locus_value)
self.cycle_val.trace_add('write', self.locus_value)
self.fexten_val.trace_add('write', self.locus_value)
vcmd = (master.register(callback_float))
vcmdi = (master.register(callback_int))
self.framel = Frame(master, width=375, height=330)
self.framel.grid(row=0, column=0, sticky="news")
self.framel.grid_propagate(0)
self.framer = Frame(master, width=310, height=330)
self.framer.grid(row=0, column=1, sticky="news")
self.framer.grid_propagate(0)
self.stock_lab = Label(self.framel, text="Stock Concentrations", font=("Arial", 10, "bold"))
self.stock_lab.grid(row=1, column=0, columnspan=3)
self.buffer_lab = Label(self.framel, text="Buffer:", width=8, anchor=W)
self.buffer_lab.grid(row=2, column=0, pady=2)
self.buffer_entry = Entry(self.framel, width=10, justify='right', textvariable=self.buffer_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.buffer_entry.grid(row=2, column=1, pady=2)
self.buffer_unit = Label(self.framel, text='x', font=("Arial", 8), width=6, anchor=W)
self.buffer_unit.grid(row=2, column=2, pady=2)
self.dntps_lab = Label(self.framel, text="DNTPs:", width=8, anchor=W)
self.dntps_lab.grid(row=3, column=0, pady=2)
self.dntps_entry = Entry(self.framel, width=10, justify='right', textvariable=self.dntps_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.dntps_entry.grid(row=3, column=1, pady=2)
self.dntps_unit = Label(self.framel, text='nmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.dntps_unit.grid(row=3, column=2, pady=2)
self.mgcl2_lab = Label(self.framel, text="MgCl₂:", width=8, anchor=W)
self.mgcl2_lab.grid(row=4, column=0, pady=2)
self.mgcl2_entry = Entry(self.framel, width=10, justify='right', textvariable=self.mgcl2_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.mgcl2_entry.grid(row=4, column=1, pady=2)
self.mgcl2_unit = Label(self.framel, text='nmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.mgcl2_unit.grid(row=4, column=2, pady=2)
self.p1_lab = Label(self.framel, text="Primer 1:", width=8, anchor=W)
self.p1_lab.grid(row=5, column=0, pady=2)
self.p1_entry = Entry(self.framel, width=10, justify='right', textvariable=self.p1_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.p1_entry.grid(row=5, column=1, pady=2)
self.p1_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p1_unit.grid(row=5, column=2, pady=2)
self.p2_lab = Label(self.framel, text="Primer 2:", width=8, anchor=W)
self.p2_lab.grid(row=6, column=0, pady=2)
self.p2_entry = Entry(self.framel, width=10, justify='right', textvariable=self.p2_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.p2_entry.grid(row=6, column=1, pady=2)
self.p2_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p2_unit.grid(row=6, column=2, pady=2)
self.p3_lab = Label(self.framel, text="Primer 3:", width=8, anchor=W)
self.p3_lab.grid(row=7, column=0, pady=2)
self.p3_entry = Entry(self.framel, width=10, justify='right', textvariable=self.p3_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.p3_entry.grid(row=7, column=1, pady=2)
self.p3_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p3_unit.grid(row=7, column=2, pady=2)
self.p4_lab = Label(self.framel, text="Primer 4:", width=8, anchor=W)
self.p4_lab.grid(row=8, column=0, pady=2)
self.p4_entry = Entry(self.framel, width=10, justify='right', textvariable=self.p4_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.p4_entry.grid(row=8, column=1, pady=2)
self.p4_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p4_unit.grid(row=8, column=2, pady=2)
self.gly_lab = Label(self.framel, text="Glycerol:", width=8, anchor=W)
self.gly_lab.grid(row=9, column=0, pady=2)
self.gly_entry = Entry(self.framel, width=10, justify='right', textvariable=self.gly_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.gly_entry.grid(row=9, column=1, pady=2)
self.gly_unit = Label(self.framel, text='%', font=("Arial", 8), width=6, anchor=W)
self.gly_unit.grid(row=9, column=2, pady=2)
self.dmso_lab = Label(self.framel, text="DMSO:", width=8, anchor=W)
self.dmso_lab.grid(row=10, column=0, pady=2)
self.dmso_entry = Entry(self.framel, width=10, justify='right', textvariable=self.dmso_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.dmso_entry.grid(row=10, column=1, pady=2)
self.dmso_unit = Label(self.framel, text='%', font=("Arial", 8), width=6, anchor=W)
self.dmso_unit.grid(row=10, column=2, pady=2)
self.taq_lab = Label(self.framel, text="Taq:", width=8, anchor=W)
self.taq_lab.grid(row=11, column=0, pady=2)
self.taq_entry = Entry(self.framel, width=10, justify='right', textvariable=self.taq_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.taq_entry.grid(row=11, column=1, pady=2)
self.taq_unit = Label(self.framel, text='U/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.taq_unit.grid(row=11, column=2, pady=2)
self.dnac_lab = Label(self.framel, text="DNA:", width=8, anchor=W)
self.dnac_lab.grid(row=12, column=0, pady=2)
self.dnac_entry = Entry(self.framel, width=10, justify='right', textvariable=self.dnac_val, validate='all',
validatecommand=(vcmd, '%P'), state=DISABLED, font=("Arial", 10))
self.dnac_entry.grid(row=12, column=1, pady=2)
self.dnac_unit = Label(self.framel, text='ng/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.dnac_unit.grid(row=12, column=2, pady=2)
self.cbs = Checkbutton(self.framel, text="Edit stock values", variable=self.check_val_stock,
command=self.stock_radio)
self.cbs.grid(row=13, column=0, columnspan=3)
self.separator = Separator(self.framel, orient=VERTICAL).grid(row=2, column=3, rowspan=12, sticky="ns",
padx=10, pady=0)
self.pcr_lab = Label(self.framel, text="PCR Concentrations", font=("Arial", 10, "bold"))
self.pcr_lab.grid(row=1, column=4, columnspan=3)
self.buffer_react_lab = Label(self.framel, text="Buffer:", width=8, anchor=W)
self.buffer_react_lab.grid(row=2, column=4)
self.buffer_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.buffer_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.buffer_react_entry.grid(row=2, column=5)
self.buffer_react_unit = Label(self.framel, text="x", font=("Arial", 8), width=6, anchor=W)
self.buffer_react_unit.grid(row=2, column=6)
self.dntps_react_lab = Label(self.framel, text="DNTPs:", width=8, anchor=W)
self.dntps_react_lab.grid(row=3, column=4)
self.dntps_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.dntps_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.dntps_react_entry.grid(row=3, column=5)
self.dntps_react_unit = Label(self.framel, text='nmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.dntps_react_unit.grid(row=3, column=6)
self.mgcl2_react_lab = Label(self.framel, text="MgCl₂:", width=8, anchor=W)
self.mgcl2_react_lab.grid(row=4, column=4)
self.mgcl2_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.mgcl2_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.mgcl2_react_entry.grid(row=4, column=5)
self.mgcl2_react_unit = Label(self.framel, text='nmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.mgcl2_react_unit.grid(row=4, column=6)
self.p1_react_lab = Label(self.framel, text="Primer 1:", width=8, anchor=W)
self.p1_react_lab.grid(row=5, column=4)
self.p1_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.primer1_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.p1_react_entry.grid(row=5, column=5)
self.p1_react_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p1_react_unit.grid(row=5, column=6)
self.p2_react_lab = Label(self.framel, text="Primer 2:", width=8, anchor=W)
self.p2_react_lab.grid(row=6, column=4)
self.p2_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.primer2_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.p2_react_entry.grid(row=6, column=5)
self.p2_react_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p2_react_unit.grid(row=6, column=6)
self.p3_react_lab = Label(self.framel, text="Primer 3:", width=8, anchor=W)
self.p3_react_lab.grid(row=7, column=4)
self.p3_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.primer3_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.p3_react_entry.grid(row=7, column=5)
self.p3_react_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p3_react_unit.grid(row=7, column=6)
self.p4_react_lab = Label(self.framel, text="Primer 4:", width=8, anchor=W)
self.p4_react_lab.grid(row=8, column=4)
self.p4_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.primer4_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.p4_react_entry.grid(row=8, column=5)
self.p4_react_unit = Label(self.framel, text='pmol/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.p4_react_unit.grid(row=8, column=6)
self.gly_react_lab = Label(self.framel, text="Glycerol:", width=8, anchor=W)
self.gly_react_lab.grid(row=9, column=4)
self.gly_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.glycerol_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.gly_react_entry.grid(row=9, column=5)
self.gly_react_unit = Label(self.framel, text='%', font=("Arial", 8), width=6, anchor=W)
self.gly_react_unit.grid(row=9, column=6)
self.dmso_react_lab = Label(self.framel, text="DMSO:", width=8, anchor=W)
self.dmso_react_lab.grid(row=10, column=4)
self.dmso_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.dmso_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.dmso_react_entry.grid(row=10, column=5)
self.dmso_react_unit = Label(self.framel, text='%', font=("Arial", 8), width=6, anchor=W)
self.dmso_react_unit.grid(row=10, column=6)
self.taq_react_lab = Label(self.framel, text="Taq:", width=8, anchor=W)
self.taq_react_lab.grid(row=11, column=4)
self.taq_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.taq_react,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.taq_react_entry.grid(row=11, column=5)
self.taq_react_unit = Label(self.framel, text='U/\u03bcL', font=("Arial", 8), width=6, anchor=W)
self.taq_react_unit.grid(row=11, column=6)
self.dna_react_lab = Label(self.framel, text="DNA:", width=8, anchor=W)
self.dna_react_lab.grid(row=12, column=4)
self.dna_react_entry = Entry(self.framel, width=7, justify='right', textvariable=self.dna_mass,
validate='all', validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.dna_react_entry.grid(row=12, column=5)
self.dna_react_unit = Label(self.framel, text='ng', font=("Arial", 8), width=6, anchor=W)
self.dna_react_unit.grid(row=12, column=6)
self.cbpcr = Checkbutton(self.framel, text="Edit PCR values", variable=self.check_val_pcr,
command=self.pcr_radio)
self.cbpcr.grid(row=13, column=4, columnspan=3)
self.separator1 = Separator(self.framel, orient=VERTICAL).grid(row=1, column=7, rowspan=13, sticky="ns",
padx=10, pady=0)
self.blank = Label(self.framer, text="")
self.blank.grid(row=7, column=0, columnspan=8)
self.select = Checkbutton(self.framer, text="Import from model", variable=self.check_val, command=self.cb_radio)
self.select.grid(row=9, column=0, columnspan=7)
self.lcb = Label(self.framer, text="Choose a Model:", width=17, anchor=W)
self.lcb.grid(row=8, column=0)
self.cb = Combobox(self.framer, textvariable=self.cb_val, values=[k for k in cycle.keys()],
font=('Arial', 8))
self.cb.configure(state=DISABLED, width=25)
self.cb.grid(row=8, column=1, pady=2, columnspan=7)
self.cycle_lab = Label(self.framer, text="PCR Cycle", font=("Arial", 10, "bold"))
self.cycle_lab.grid(row=1, column=0, columnspan=8)
self.init_denat_lab = Label(self.framer, text="Initial Denaturation:", width=17, anchor=W)
self.init_denat_lab.grid(row=2, column=0, pady=2)
self.init_denat_entry = Entry(self.framer, width=4, justify='right', validate='all', textvariable=self.id_val,
validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.init_denat_entry.grid(row=2, column=1, pady=2)
self.init_denat_time = Label(self.framer, text='s at', font=("Arial", 8))
self.init_denat_time.grid(row=2, column=2, pady=2)
self.init_denat_entry2 = Entry(self.framer, width=4, justify='right', validate='all', textvariable=self.id_val2,
validatecommand=(vcmd, '%P'), font=("Arial", 10))
self.init_denat_entry2.grid(row=2, column=3, pady=2)
self.init_denat_temp = Label(self.framer, text='\u00b0C', font=("Arial", 8))
self.init_denat_temp.grid(row=2, column=4, pady=2)
self.denat_lab = Label(self.framer, text="Denaturation:", width=17, anchor=W)
self.denat_lab.grid(row=3, column=0, pady=2)
self.denat_entry = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.d_val, font=("Arial", 10))
self.denat_entry.grid(row=3, column=1, pady=2)
self.denat_time = Label(self.framer, text='s at', font=("Arial", 8))
self.denat_time.grid(row=3, column=2, pady=2)
self.denat_entry2 = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.d_val2, font=("Arial", 10))
self.denat_entry2.grid(row=3, column=3, pady=2)
self.denat_temp = Label(self.framer, text='\u00b0C', font=("Arial", 8))
self.denat_temp.grid(row=3, column=4, pady=2)
self.anneal_lab = Label(self.framer, text="Annealing:", width=17, anchor=W)
self.anneal_lab.grid(row=4, column=0, pady=2)
self.anneal_entry = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.anneal_val, font=("Arial", 10))
self.anneal_entry.grid(row=4, column=1, pady=2)
self.anneal_time = Label(self.framer, text='s at', font=("Arial", 8))
self.anneal_time.grid(row=4, column=2, pady=2)
self.anneal_entry2 = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.anneal_val2, font=("Arial", 10))
self.anneal_entry2.grid(row=4, column=3, pady=2)
self.anneal_temp = Label(self.framer, text='\u00b0C', font=("Arial", 8))
self.anneal_temp.grid(row=4, column=4, pady=2)
self.cycles_entry = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.cycle_val, font=("Arial", 10))
self.cycles_entry.grid(row=4, column=6)
self.cycles_lab = Label(self.framer, text='x')
self.cycles_lab.grid(row=4, column=7)
self.exten_lab = Label(self.framer, text="Extension:", width=17, anchor=W)
self.exten_lab.grid(row=5, column=0, pady=2)
self.exten_entry = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.exten_val, font=("Arial", 10))
self.exten_entry.grid(row=5, column=1, pady=2)
self.exten_time = Label(self.framer, text='s at', font=("Arial", 8))
self.exten_time.grid(row=5, column=2, pady=2)
self.exten_entry2 = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.exten_val2, font=("Arial", 10))
self.exten_entry2.grid(row=5, column=3, pady=2)
self.exten_temp = Label(self.framer, text='\u00b0C', font=("Arial", 8))
self.exten_temp.grid(row=5, column=4, pady=2)
img = Image.open(join(datadir, "images/brace.png"))
img = img.resize((20, 75), Image.ANTIALIAS)
img = ImageTk.PhotoImage(img)
self.brackets = Label(self.framer, image=img)
self.brackets.image = img
self.brackets.grid(row=3, column=5, rowspan=3)
self.fexten_lab = Label(self.framer, text="Final Extension:", width=17, anchor=W)
self.fexten_lab.grid(row=6, column=0, pady=2)
self.fexten_entry = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.fexten_val, font=("Arial", 10))
self.fexten_entry.grid(row=6, column=1, pady=2)
self.fexten_time = Label(self.framer, text='m at', font=("Arial", 8))
self.fexten_time.grid(row=6, column=2, pady=2)
self.fexten_entry2 = Entry(self.framer, width=4, justify='right', validate='all', validatecommand=(vcmd, '%P'),
textvariable=self.fexten_val2, font=("Arial", 10))
self.fexten_entry2.grid(row=6, column=3, pady=2)
self.fexten_temp = Label(self.framer, text='\u00b0C', font=("Arial", 8))
self.fexten_temp.grid(row=6, column=4, pady=2)
self.duration_lab = Label(self.framer, text="Minimum Run Time:", width=17, anchor=W)
self.duration_lab.grid(row=10, column=0, pady=2)
self.duration = Label(self.framer, textvariable=self.total_time, width=17, anchor=W)
self.duration.grid(row=10, column=1, columnspan=7)
self.separator2 = Separator(self.framer, orient=HORIZONTAL).grid(row=11, column=0, columnspan=8, padx=0,
pady=2, sticky="ew")
self.react_num_lab = Label(self.framer, text="PCR Reactions Number:", width=22, anchor=W)
self.react_num_lab.grid(row=13, column=0, columnspan=2)
self.react_num_entry = Entry(self.framer, width=15, justify='right', validate='all',
validatecommand=(vcmdi, '%P'), textvariable=self.reaction_number,
font=("Arial", 10))
self.react_num_entry.grid(row=13, column=2, columnspan=5, pady=(1, 5))
self.react_vol_lab = Label(self.framer, text="Reaction Volume:", width=22, anchor=W)
self.react_vol_lab.grid(row=12, column=0, columnspan=2)
self.react_vol_entry = Entry(self.framer, width=15, justify='right', validate='all',
validatecommand=(vcmd, '%P'), textvariable=self.volume, font=("Arial", 10))
self.react_vol_entry.grid(row=12, column=2, columnspan=5)
self.react_vol_unit = Label(self.framer, text='\u03bcL', font=("Arial", 8), width=2, anchor=W)
self.react_vol_unit.grid(row=12, column=7)
self.button = Button(self.framer, text="View Mix", command=lambda: mix(self))
self.button.grid(row=14, column=0, columnspan=2)
self.exit = Button(self.framer, text="Close", command=exit)
self.exit.grid(row=14, column=2, columnspan=5)
def locus_value(self, *args):
if self.check_val.get() == 1:
data = cycle[self.cb_val.get()]
self.fexten_val.set(data['Final Extension']['m'])
self.fexten_val2.set(data['Final Extension']['C'])
self.id_val.set(data['Initial Denaturation']['s'])
self.id_val2.set(data['Initial Denaturation']['C'])
self.d_val.set(data['Denaturation']['s'])
self.d_val2.set(data['Denaturation']['C'])
self.anneal_val.set(data['Annealing']['s'])
self.anneal_val2.set(data['Annealing']['C'])
self.exten_val.set(data['Extension']['s'])
self.exten_val2.set(data['Extension']['C'])
self.cycle_val.set(data['Number of Cycles'])
self.total_time.set(duration(self))
else:
self.id_val.set(self.init_denat_entry.get())
self.d_val.set(self.denat_entry.get())
self.anneal_val.set(self.anneal_entry.get())
self.exten_val.set(self.exten_entry.get())
self.cycle_val.set(self.cycles_entry.get())
self.fexten_val.set(self.fexten_entry.get())
self.total_time.set(duration(self))
for arg in args:
del arg
def cb_radio(self):
"""
Verify the Checkbutton variable values and handles combobox entry state accordingly.
"""
if self.check_val.get() == 0:
self.cb.configure(state=DISABLED)
else:
self.cb.configure(state="readonly")
def stock_radio(self):
"""
Verify the Checkbutton variable values and handles stock entries accordingly.
"""
if self.check_val_stock.get() == 0:
self.buffer_entry.configure(state=DISABLED)
self.dntps_entry.configure(state=DISABLED)
self.mgcl2_entry.configure(state=DISABLED)
self.p1_entry.configure(state=DISABLED)
self.p2_entry.configure(state=DISABLED)
self.p3_entry.configure(state=DISABLED)
self.p4_entry.configure(state=DISABLED)
self.gly_entry.configure(state=DISABLED)
self.dmso_entry.configure(state=DISABLED)
self.taq_entry.configure(state=DISABLED)
self.dnac_entry.configure(state=DISABLED)
else:
self.buffer_entry.configure(state=NORMAL)
self.dntps_entry.configure(state=NORMAL)
self.mgcl2_entry.configure(state=NORMAL)
self.p1_entry.configure(state=NORMAL)
self.p2_entry.configure(state=NORMAL)
self.p3_entry.configure(state=NORMAL)
self.p4_entry.configure(state=NORMAL)
self.gly_entry.configure(state=NORMAL)
self.dmso_entry.configure(state=NORMAL)
self.taq_entry.configure(state=NORMAL)
self.dnac_entry.configure(state=NORMAL)
def pcr_radio(self):
"""
Verify the Checkbutton variable values and handles stock entries accordingly.
"""
if self.check_val_pcr.get() == 0:
self.buffer_react_entry.configure(state=DISABLED)
self.dntps_react_entry.configure(state=DISABLED)
self.mgcl2_react_entry.configure(state=DISABLED)
self.p1_react_entry.configure(state=DISABLED)
self.p2_react_entry.configure(state=DISABLED)
self.p3_react_entry.configure(state=DISABLED)
self.p4_react_entry.configure(state=DISABLED)
self.gly_react_entry.configure(state=DISABLED)
self.dmso_react_entry.configure(state=DISABLED)
self.taq_react_entry.configure(state=DISABLED)
self.dna_react_entry.configure(state=DISABLED)
else:
self.buffer_react_entry.configure(state=NORMAL)
self.dntps_react_entry.configure(state=NORMAL)
self.mgcl2_react_entry.configure(state=NORMAL)
self.p1_react_entry.configure(state=NORMAL)
self.p2_react_entry.configure(state=NORMAL)
self.p3_react_entry.configure(state=NORMAL)
self.p4_react_entry.configure(state=NORMAL)
self.gly_react_entry.configure(state=NORMAL)
self.dmso_react_entry.configure(state=NORMAL)
self.taq_react_entry.configure(state=NORMAL)
self.dna_react_entry.configure(state=NORMAL)