diff --git a/pdb2pqr/dat/PATCHES.xml b/pdb2pqr/dat/PATCHES.xml
index 2069701c..4cd04c86 100644
--- a/pdb2pqr/dat/PATCHES.xml
+++ b/pdb2pqr/dat/PATCHES.xml
@@ -579,7 +579,7 @@ OF THE POSSIBILITY OF SUCH DAMAGE.
   </patch>
   <patch>
      <name>DT5</name>
-     <applyto>T</applyto>
+     <applyto>DT</applyto>
      <remove>O1P</remove>
      <remove>P</remove>
      <remove>O2P</remove>
diff --git a/tests/patches_test.py b/tests/patches_test.py
new file mode 100644
index 00000000..5c2341d0
--- /dev/null
+++ b/tests/patches_test.py
@@ -0,0 +1,20 @@
+from pathlib import Path
+
+from pdb2pqr.definitions import Definition
+
+
+def test_dt5_patch_applies_to_dt_reference() -> None:
+    dat_dir = Path(__file__).resolve().parents[1] / "pdb2pqr" / "dat"
+
+    with (
+        (dat_dir / "AA.xml").open() as aa_file,
+        (dat_dir / "NA.xml").open() as na_file,
+        (dat_dir / "PATCHES.xml").open() as patch_file,
+    ):
+        definition = Definition(aa_file, na_file, patch_file)
+
+    assert "DT" in definition.map
+    assert "DT5" in definition.map
+    assert "P" not in definition.map["DT5"].map
+    assert "O1P" not in definition.map["DT5"].map
+    assert "H5T" in definition.map["DT5"].map