Demultiplexing sample #10
Description
I am trying to demultiplex the samples before the analysis to get samples first. This is the code:
java -jar /rsstu/users/r/rrellan/sara/nirwan_backup/ntanduk/BRBseqTools.jar Demultiplex -r1 /rsstu/users/r/rrellan/sara/RNA_Sequencing_raw/BZea_CLY23D1/NVS205B_RellanAlvarez/BZeaR2_S1_L004_R1_001.fastq.gz -r2 /rsstu/users/r/rrellan/sara/RNA_Sequencing_raw/BZea_CLY23D1/NVS205B_RellanAlvarez/BZeaR2_S1_L004_R2_001.fastq.gz -c /share/maize/ntanduk/BZea/rnaseq/barcodes.txt -p BU -UMI 14
this is my barcodes.txt
(base) [ntanduk@login02 rnaseq]$ more barcodes.txt
Name B1
B73_1 TACACTATAGCTAG
B73_2 CAGACATGATAGAG
B73_3 TCTTGAACGACTAA
B73_4 ACCAACTTAGACAA
B73_5 CCATATTGAAGCAC
B73_6 TTCATGGTAGAGAG
B73_7 CACGTAGCTAGACG
B73_8 ATACGATGCTGCGC
B73_9 ACTTAAGGTCCAAC
B73_10 CTCTCAAGTCCTCC
This is my error:
Error while parsing R1 FastQ file
Read found in FastQ has length 151 while barcode pattern has length 28
I have UMI in my samples.
Any help is appreciated