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README.md

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@@ -41,10 +41,9 @@ pip install ibis-genome
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## Ibis coassemble
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Snakemake pipeline to discover coassembly sample clusters based on co-occurrence of single-copy marker genes, excluding those genes present in reference genomes (e.g. previously recovered genomes).
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Creates graph with samples as nodes and the number of overlapping sequences provided by SingleM.
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The taxa of the considered sequences can be filtered to target a specific taxon (e.g. the phylum Planctomycetota).
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The graph is clustered using the Girvan-Newman algorithm to provide sample groupings.
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Aviary assemble/recover commands are generated based on proposed coassemblies.
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Assembly and recovery can be run directly, or the coassemblies with differential-abudance-binning samples can be run in the tool of your choice.
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Aviary assemble/recover commands are also generated based on proposed coassemblies.
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Optionally, reads can be mapped to the matched bins with only unmapped reads being assembled.
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Paired end reads of form reads_1.1.fq, reads_1_1.fq and reads_1_R1.fq are automatically detected and matched to their basename.
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## Ibis update
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Applies further processing to a previous Ibis coassemble run: downloading SRA reads, generating unmapped reads files, and/or running Aviary commands.
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Applies further processing to a previous Ibis coassemble run: downloading SRA reads, generating unmapped reads files, and/or running assembly/recovery commands.
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```bash
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# Example: update previous run to download SRA reads

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